Els Pardon

Affiliations

Structural Biology Brussels, Vrije Universiteit Brussel, B-1050 Brussels, Belgium.

Publications (27)

Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Joël S Bloch , Somnath Mukherjee , Julia Kowal , Ekaterina V Filippova , Martina Niederer , Els Pardon , Jan Steyaert , Anthony A Kossiakoff , Kaspar P Locher

Structural basis of purine nucleotide inhibition of human uncoupling protein 1.

Science advances

Avec: Scott A Jones , Prerana Gogoi , Jonathan J Ruprecht , Martin S King , Yang Lee , Thomas Zögg , Els Pardon , Deepak Chand , Stefan Steimle , Danielle M Copeman , Camila A Cotrim , Jan Steyaert , Paul G Crichton , Vera Moiseenkova-Bell , Edmund R S Kunji

Bypassing the Need for Cell Permeabilization: Nanobody CDR3 Peptide Improves Binding on Living Bacteria.

Bioconjugate chemistry

Avec: A Breine , K Van Holsbeeck , C Martin , S Gonzalez , M Mannes , E Pardon , J Steyaert , H Remaut , S Ballet , C Van der Henst

Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domain.

Nature communications

Avec: Arum Wu , David Salom , John D Hong , Aleksander Tworak , Kohei Watanabe , Els Pardon , Jan Steyaert , Hideki Kandori , Kota Katayama , Philip D Kiser , Krzysztof Palczewski

Structure and function of the EA1 surface layer of Bacillus anthracis.

Nature communications

Avec: Adrià Sogues , Antonella Fioravanti , Wim Jonckheere , Els Pardon , Jan Steyaert , Han Remaut

Structure-based design of nanobodies that inhibit seeding of Alzheimer's patient-extracted tau fibrils.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Romany Abskharon , Hope Pan , Michael R Sawaya , Paul M Seidler , Eileen J Olivares , Yu Chen , Kevin A Murray , Jeffrey Zhang , Carter Lantz , Megan Bentzel , David R Boyer , Duilio Cascio , Binh A Nguyen , Ke Hou , Xinyi Cheng , Els Pardon , Christopher K Williams , Alissa L Nana , Harry V Vinters , Salvatore Spina , Lea T Grinberg , William W Seeley , Jan Steyaert , Charles G Glabe , Rachel R Ogorzalek Loo , Joseph A Loo , David S Eisenberg

Allosteric nanobodies to study the interactions between SOS1 and RAS.

Nature communications

Avec: Baptiste Fischer , Tomasz Uchański , Aidana Sheryazdanova , Simon Gonzalez , Alexander N Volkov , Elke Brosens , Thomas Zögg , Valentina Kalichuk , Steven Ballet , Wim Versées , Anna A Sablina , Els Pardon , Alexandre Wohlkönig , Jan Steyaert

The G-Protein Rab5A Activates VPS34 Complex II, a Class III PI3K, by a Dual Regulatory Mechanism.

Biophysical journal

Avec: Thomas C Buckles , Yohei Ohashi , Shirley Tremel , Stephen H McLaughlin , Els Pardon , Jan Steyaert , Moshe T Gordon , Roger L Williams , Joseph J Falke

Modular transient nanoclustering of activated β2-adrenergic receptors revealed by single-molecule tracking of conformation-specific nanobodies.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Rachel S Gormal , Pranesh Padmanabhan , Ravikiran Kasula , Adekunle T Bademosi , Sean Coakley , Jean Giacomotto , Ailisa Blum , Merja Joensuu , Tristan P Wallis , Harriet P Lo , Srikanth Budnar , James Rae , Charles Ferguson , Michele Bastiani , Walter G Thomas , Els Pardon , Jan Steyaert , Alpha S Yap , Geoffrey J Goodhill , Massimo A Hilliard , Robert G Parton , Frédéric A Meunier

Structure of the phosphoinositide 3-kinase (PI3K) p110γ-p101 complex reveals molecular mechanism of GPCR activation.

Science advances

Avec: Manoj K Rathinaswamy , Udit Dalwadi , Kaelin D Fleming , Carson Adams , Jordan T B Stariha , Els Pardon , Minkyung Baek , Oscar Vadas , Frank DiMaio , Jan Steyaert , Scott D Hansen , Calvin K Yip , John E Burke

Structure, substrate recognition and initiation of hyaluronan synthase.

Nature

Avec: Finn P Maloney , Jeremi Kuklewicz , Robin A Corey , Yunchen Bi , Ruoya Ho , Lukasz Mateusiak , Els Pardon , Jan Steyaert , Phillip J Stansfeld , Jochen Zimmer

Plasticity of the binding pocket in peptide transporters underpins promiscuous substrate recognition.

Cell reports

Avec: Vadim Kotov , Maxime Killer , Katharina E J Jungnickel , Jian Lei , Giada Finocchio , Josi Steinke , Kim Bartels , Jan Strauss , Florine Dupeux , Anne-Sophie Humm , Irina Cornaciu , José A Márquez , Els Pardon , Jan Steyaert , Christian Löw

In vivo and in vitro characterizations of melibiose permease (MelB) conformation-dependent nanobodies reveal sugar-binding mechanisms.

The Journal of biological chemistry

Avec: Satoshi Katsube , Katleen Willibal , Sangama Vemulapally , Parameswaran Hariharan , Elena Tikhonova , Els Pardon , H Ronald Kaback , Jan Steyaert , Lan Guan

Mobile barrier mechanisms for Na

bioRxiv : the preprint server for biology

Avec: Parameswaran Hariharan , Yuqi Shi , Satoshi Katsube , Katleen Willibal , Nathan D Burrows , Patrick Mitchell , Amirhossein Bakhtiiari , Samantha Stanfield , Els Pardon , H Ronald Kaback , Ruibin Liang , Jan Steyaert , Rosa Viner , Lan Guan

Receptor-recognition and antiviral mechanisms of retrovirus-derived human proteins.

Nature structural & molecular biology

Avec: Shashank Khare , Miryam I Villalba , Juan C Canul-Tec , Arantza Balsebre Cajiao , Anand Kumar , Marija Backovic , Felix A Rey , Els Pardon , Jan Steyaert , Camilo Perez , Nicolas Reyes

Rapid small-scale nanobody-assisted purification of ryanodine receptors for cryo-EM.

The Journal of biological chemistry

Avec: Chenyao Li , Katrien Willegems , Tomasz Uchański , Els Pardon , Jan Steyaert , Rouslan G Efremov

Structural insights into Frizzled3 through nanobody modulators.

Nature communications

Avec: James Hillier , Yuguang Zhao , Loic Carrique , Tomas Malinauskas , Reinis R Ruza , Tao-Hsin Chang , Gangshun Yi , Helen M E Duyvesteyn , Jing Yu , Weixian Lu , Els Pardon , Jan Steyaert , Yanan Zhu , Tao Ni , E Yvonne Jones

An improved yeast surface display platform for the screening of nanobody immune libraries.

Scientific reports

Avec: Tomasz Uchański , Thomas Zögg , Jie Yin , Daopeng Yuan , Alexandre Wohlkönig , Baptiste Fischer , Daniel M Rosenbaum , Brian K Kobilka , Els Pardon , Jan Steyaert

Structural evidence for the critical role of the prion protein hydrophobic region in forming an infectious prion.

PLoS pathogens

Avec: Romany Abskharon , Fei Wang , Alexandre Wohlkonig , Juxin Ruan , Sameh Soror , Gabriele Giachin , Els Pardon , Wenquan Zou , Giuseppe Legname , Jiyan Ma , Jan Steyaert

Cryo-EM structure of the human α1β3γ2 GABA

Nature

Avec: Duncan Laverty , Rooma Desai , Tomasz Uchański , Simonas Masiulis , Wojciech J Stec , Tomas Malinauskas , Jasenko Zivanov , Els Pardon , Jan Steyaert , Keith W Miller , A Radu Aricescu

Structures of ligand-occupied β-Klotho complexes reveal a molecular mechanism underlying endocrine FGF specificity and activity.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Ekaterina S Kuzina , Peter Man-Un Ung , Jyotidarsini Mohanty , Francisco Tome , Jungyuen Choi , Els Pardon , Jan Steyaert , Irit Lax , Avner Schlessinger , Joseph Schlessinger , Sangwon Lee

Modulation of the

eLife

Avec: Marijke Brams , Cedric Govaerts , Kumiko Kambara , Kerry L Price , Radovan Spurny , Anant Gharpure , Els Pardon , Genevieve L Evans , Daniel Bertrand , Sarah Cr Lummis , Ryan E Hibbs , Jan Steyaert , Chris Ulens

Nanobodies to study protein conformational states.

Current opinion in structural biology

Avec: Tomasz Uchański , Els Pardon , Jan Steyaert

Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.

Nature communications

Avec: Maud Sigoillot , Marie Overtus , Magdalena Grodecka , Daniel Scholl , Abel Garcia-Pino , Toon Laeremans , Lihua He , Els Pardon , Ellen Hildebrandt , Ina Urbatsch , Jan Steyaert , John R Riordan , Cedric Govaerts

Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM.

Nature methods

Avec: Tomasz Uchański , Simonas Masiulis , Baptiste Fischer , Valentina Kalichuk , Uriel López-Sánchez , Eleftherios Zarkadas , Miriam Weckener , Andrija Sente , Philip Ward , Alexandre Wohlkönig , Thomas Zögg , Han Remaut , James H Naismith , Hugues Nury , Wim Vranken , A Radu Aricescu , Els Pardon , Jan Steyaert

Nano-scale resolution of native retinal rod disk membranes reveals differences in lipid composition.

The Journal of cell biology

Avec: Christopher L Sander , Avery E Sears , Antonio F M Pinto , Elliot H Choi , Shirin Kahremany , Fangyuan Gao , David Salom , Hui Jin , Els Pardon , Susie Suh , Zhiqian Dong , Jan Steyaert , Alan Saghatelian , Dorota Skowronska-Krawczyk , Philip D Kiser , Krzysztof Palczewski

Nanobodies as allosteric modulators of Parkinson's disease-associated LRRK2.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Ranjan K Singh , Ahmed Soliman , Giambattista Guaitoli , Eliza Störmer , Felix von Zweydorf , Thomas Dal Maso , Asmaa Oun , Laura Van Rillaer , Sven H Schmidt , Deep Chatterjee , Joshua A David , Els Pardon , Thomas U Schwartz , Stefan Knapp , Eileen J Kennedy , Jan Steyaert , Friedrich W Herberg , Arjan Kortholt , Christian Johannes Gloeckner , Wim Versées

Réseau de co-auteurs

Jan Steyaert 26 collaborations

Tomasz Uchański 6 collaborations

Thomas Zögg 4 collaborations

Baptiste Fischer 3 collaborations

Alexandre Wohlkönig 3 collaborations

David Salom 2 collaborations

Philip D Kiser 2 collaborations

Krzysztof Palczewski 2 collaborations

Han Remaut 2 collaborations

Romany Abskharon 2 collaborations

Valentina Kalichuk 2 collaborations

Wim Versées 2 collaborations

Satoshi Katsube 2 collaborations

Katleen Willibal 2 collaborations

Parameswaran Hariharan 2 collaborations

H Ronald Kaback 2 collaborations

Lan Guan 2 collaborations

Tomas Malinauskas 2 collaborations

Simonas Masiulis 2 collaborations

A Radu Aricescu 2 collaborations

Cedric Govaerts 2 collaborations

Joël S Bloch 1 collaboration

Somnath Mukherjee 1 collaboration

Julia Kowal 1 collaboration

Ekaterina V Filippova 1 collaboration

Martina Niederer 1 collaboration

Anthony A Kossiakoff 1 collaboration

Kaspar P Locher 1 collaboration

Scott A Jones 1 collaboration

Prerana Gogoi 1 collaboration

Jonathan J Ruprecht 1 collaboration

Martin S King 1 collaboration

Yang Lee 1 collaboration

Deepak Chand 1 collaboration

Stefan Steimle 1 collaboration

Danielle M Copeman 1 collaboration

Camila A Cotrim 1 collaboration

Paul G Crichton 1 collaboration

Vera Moiseenkova-Bell 1 collaboration

Edmund R S Kunji 1 collaboration

A Breine 1 collaboration

K Van Holsbeeck 1 collaboration

C Martin 1 collaboration

S Gonzalez 1 collaboration

M Mannes 1 collaboration

E Pardon 1 collaboration

J Steyaert 1 collaboration

H Remaut 1 collaboration

S Ballet 1 collaboration

C Van der Henst 1 collaboration

Arum Wu 1 collaboration

John D Hong 1 collaboration

Aleksander Tworak 1 collaboration

Kohei Watanabe 1 collaboration

Hideki Kandori 1 collaboration

Kota Katayama 1 collaboration

Adrià Sogues 1 collaboration

Antonella Fioravanti 1 collaboration

Wim Jonckheere 1 collaboration

Hope Pan 1 collaboration

Michael R Sawaya 1 collaboration

Paul M Seidler 1 collaboration

Eileen J Olivares 1 collaboration

Yu Chen 1 collaboration

Kevin A Murray 1 collaboration

Jeffrey Zhang 1 collaboration

Carter Lantz 1 collaboration

Megan Bentzel 1 collaboration

David R Boyer 1 collaboration

Duilio Cascio 1 collaboration

Binh A Nguyen 1 collaboration

Ke Hou 1 collaboration

Xinyi Cheng 1 collaboration

Christopher K Williams 1 collaboration

Alissa L Nana 1 collaboration

Harry V Vinters 1 collaboration

Salvatore Spina 1 collaboration

Lea T Grinberg 1 collaboration

William W Seeley 1 collaboration

Charles G Glabe 1 collaboration

Rachel R Ogorzalek Loo 1 collaboration

Joseph A Loo 1 collaboration

David S Eisenberg 1 collaboration

Aidana Sheryazdanova 1 collaboration

Simon Gonzalez 1 collaboration

Alexander N Volkov 1 collaboration

Elke Brosens 1 collaboration

Steven Ballet 1 collaboration

Anna A Sablina 1 collaboration

Thomas C Buckles 1 collaboration

Yohei Ohashi 1 collaboration

Shirley Tremel 1 collaboration

Stephen H McLaughlin 1 collaboration

Moshe T Gordon 1 collaboration

Roger L Williams 1 collaboration

Joseph J Falke 1 collaboration

Rachel S Gormal 1 collaboration

Pranesh Padmanabhan 1 collaboration

Ravikiran Kasula 1 collaboration

Adekunle T Bademosi 1 collaboration

Sean Coakley 1 collaboration

Jean Giacomotto 1 collaboration

Ailisa Blum 1 collaboration

Merja Joensuu 1 collaboration

Tristan P Wallis 1 collaboration

Harriet P Lo 1 collaboration

Srikanth Budnar 1 collaboration

James Rae 1 collaboration

Charles Ferguson 1 collaboration

Michele Bastiani 1 collaboration

Walter G Thomas 1 collaboration

Alpha S Yap 1 collaboration

Geoffrey J Goodhill 1 collaboration

Massimo A Hilliard 1 collaboration

Robert G Parton 1 collaboration

Frédéric A Meunier 1 collaboration

Manoj K Rathinaswamy 1 collaboration

Udit Dalwadi 1 collaboration

Kaelin D Fleming 1 collaboration

Carson Adams 1 collaboration

Jordan T B Stariha 1 collaboration

Minkyung Baek 1 collaboration

Oscar Vadas 1 collaboration

Frank DiMaio 1 collaboration

Scott D Hansen 1 collaboration

Calvin K Yip 1 collaboration

John E Burke 1 collaboration

Finn P Maloney 1 collaboration

Jeremi Kuklewicz 1 collaboration

Robin A Corey 1 collaboration

Yunchen Bi 1 collaboration

Ruoya Ho 1 collaboration

Lukasz Mateusiak 1 collaboration

Phillip J Stansfeld 1 collaboration

Jochen Zimmer 1 collaboration

Vadim Kotov 1 collaboration

Maxime Killer 1 collaboration

Katharina E J Jungnickel 1 collaboration

Jian Lei 1 collaboration

Giada Finocchio 1 collaboration

Josi Steinke 1 collaboration

Kim Bartels 1 collaboration

Jan Strauss 1 collaboration

Florine Dupeux 1 collaboration

Anne-Sophie Humm 1 collaboration

Irina Cornaciu 1 collaboration

José A Márquez 1 collaboration

Christian Löw 1 collaboration

Sangama Vemulapally 1 collaboration

Elena Tikhonova 1 collaboration

Yuqi Shi 1 collaboration

Nathan D Burrows 1 collaboration

Patrick Mitchell 1 collaboration

Amirhossein Bakhtiiari 1 collaboration

Samantha Stanfield 1 collaboration

Ruibin Liang 1 collaboration

Rosa Viner 1 collaboration

Shashank Khare 1 collaboration

Miryam I Villalba 1 collaboration

Juan C Canul-Tec 1 collaboration

Arantza Balsebre Cajiao 1 collaboration

Anand Kumar 1 collaboration

Marija Backovic 1 collaboration

Felix A Rey 1 collaboration

Camilo Perez 1 collaboration

Nicolas Reyes 1 collaboration

Chenyao Li 1 collaboration

Katrien Willegems 1 collaboration

Rouslan G Efremov 1 collaboration

James Hillier 1 collaboration

Yuguang Zhao 1 collaboration

Loic Carrique 1 collaboration

Reinis R Ruza 1 collaboration

Tao-Hsin Chang 1 collaboration

Gangshun Yi 1 collaboration

Helen M E Duyvesteyn 1 collaboration

Jing Yu 1 collaboration

Weixian Lu 1 collaboration

Yanan Zhu 1 collaboration

Tao Ni 1 collaboration

E Yvonne Jones 1 collaboration

Jie Yin 1 collaboration

Daopeng Yuan 1 collaboration

Daniel M Rosenbaum 1 collaboration

Brian K Kobilka 1 collaboration

Fei Wang 1 collaboration

Alexandre Wohlkonig 1 collaboration

Juxin Ruan 1 collaboration

Sameh Soror 1 collaboration

Gabriele Giachin 1 collaboration

Wenquan Zou 1 collaboration

Giuseppe Legname 1 collaboration

Jiyan Ma 1 collaboration

Duncan Laverty 1 collaboration

Rooma Desai 1 collaboration

Wojciech J Stec 1 collaboration

Jasenko Zivanov 1 collaboration

Keith W Miller 1 collaboration

Ekaterina S Kuzina 1 collaboration

Peter Man-Un Ung 1 collaboration

Jyotidarsini Mohanty 1 collaboration

Francisco Tome 1 collaboration

Jungyuen Choi 1 collaboration

Irit Lax 1 collaboration

Avner Schlessinger 1 collaboration

Joseph Schlessinger 1 collaboration

Sangwon Lee 1 collaboration

Marijke Brams 1 collaboration

Kumiko Kambara 1 collaboration

Kerry L Price 1 collaboration

Radovan Spurny 1 collaboration

Anant Gharpure 1 collaboration

Genevieve L Evans 1 collaboration

Daniel Bertrand 1 collaboration

Sarah Cr Lummis 1 collaboration

Ryan E Hibbs 1 collaboration

Chris Ulens 1 collaboration

Maud Sigoillot 1 collaboration

Marie Overtus 1 collaboration

Magdalena Grodecka 1 collaboration

Daniel Scholl 1 collaboration

Abel Garcia-Pino 1 collaboration

Toon Laeremans 1 collaboration

Lihua He 1 collaboration

Ellen Hildebrandt 1 collaboration

Ina Urbatsch 1 collaboration

John R Riordan 1 collaboration

Uriel López-Sánchez 1 collaboration

Eleftherios Zarkadas 1 collaboration

Miriam Weckener 1 collaboration

Andrija Sente 1 collaboration

Philip Ward 1 collaboration

James H Naismith 1 collaboration

Hugues Nury 1 collaboration

Wim Vranken 1 collaboration

Christopher L Sander 1 collaboration

Avery E Sears 1 collaboration

Antonio F M Pinto 1 collaboration

Elliot H Choi 1 collaboration

Shirin Kahremany 1 collaboration

Fangyuan Gao 1 collaboration

Hui Jin 1 collaboration

Susie Suh 1 collaboration

Zhiqian Dong 1 collaboration

Alan Saghatelian 1 collaboration

Dorota Skowronska-Krawczyk 1 collaboration

Ranjan K Singh 1 collaboration

Ahmed Soliman 1 collaboration

Giambattista Guaitoli 1 collaboration

Eliza Störmer 1 collaboration

Felix von Zweydorf 1 collaboration

Thomas Dal Maso 1 collaboration

Asmaa Oun 1 collaboration

Laura Van Rillaer 1 collaboration

Sven H Schmidt 1 collaboration

Deep Chatterjee 1 collaboration

Joshua A David 1 collaboration

Thomas U Schwartz 1 collaboration

Stefan Knapp 1 collaboration

Eileen J Kennedy 1 collaboration

Friedrich W Herberg 1 collaboration

Arjan Kortholt 1 collaboration

Christian Johannes Gloeckner 1 collaboration