Nano-scale resolution of native retinal rod disk membranes reveals differences in lipid composition.

ATP-Binding Cassette Transporters / genetics Alcohol Oxidoreductases / genetics Animals Cattle Cell Membrane / metabolism Eye Proteins / immunology Lipidomics Membrane Lipids / metabolism Membrane Proteins / immunology Mice, Inbred BALB C Mice, Knockout Microscopy, Electron, Transmission Nanotechnology Peripherins / metabolism Retinal Rod Photoreceptor Cells / metabolism Rhodopsin / metabolism Single-Domain Antibodies / immunology Tetraspanins / metabolism

Journal

The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356

Informations de publication

Date de publication:
02 08 2021
Historique:
received: 11 01 2021
revised: 26 04 2021
accepted: 18 05 2021
entrez: 16 6 2021
pubmed: 17 6 2021
medline: 21 10 2021
Statut: ppublish

Résumé

Photoreceptors rely on distinct membrane compartments to support their specialized function. Unlike protein localization, identification of critical differences in membrane content has not yet been expanded to lipids, due to the difficulty of isolating domain-specific samples. We have overcome this by using SMA to coimmunopurify membrane proteins and their native lipids from two regions of photoreceptor ROS disks. Each sample's copurified lipids were subjected to untargeted lipidomic and fatty acid analysis. Extensive differences between center (rhodopsin) and rim (ABCA4 and PRPH2/ROM1) samples included a lower PC to PE ratio and increased LC- and VLC-PUFAs in the center relative to the rim region, which was enriched in shorter, saturated FAs. The comparatively few differences between the two rim samples likely reflect specific protein-lipid interactions. High-resolution profiling of the ROS disk lipid composition gives new insights into how intricate membrane structure and protein activity are balanced within the ROS, and provides a model for future studies of other complex cellular structures.

Identifiants

DOI: 10.1083/jcb.202101063 PMID: 34132745 PMC: PMC8240855
pubmed: 34132745
pii: 212388
doi: 10.1083/jcb.202101063
pmc: PMC8240855
pii:
doi:

Substances chimiques

ATP-Binding Cassette Transporters 0
Abca4 protein, mouse 0
Eye Proteins 0
Membrane Lipids 0
Membrane Proteins 0
Peripherins 0
Single-Domain Antibodies 0
Tetraspanins 0
Rhodopsin 9009-81-8
Alcohol Oxidoreductases EC 1.1.-
Rdh8 protein, mouse EC 1.1.1.105

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NEI NIH HHS
ID : P30 EY011373
Pays : United States
Organisme : NEI NIH HHS
ID : T32 EY007157
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY030873
Pays : United States
Organisme : NEI NIH HHS
ID : T32 EY024236
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008803
Pays : United States
Organisme : NEI NIH HHS
ID : R24 EY027283
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007250
Pays : United States
Organisme : NIH HHS
ID : S10 OD021815
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY009339
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY019312
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103311
Pays : United States
Organisme : NEI NIH HHS
ID : F30 EY029136
Pays : United States
Organisme : BLRD VA
ID : I01 BX004939
Pays : United States

Informations de copyright

© 2021 Sander et al.

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Auteurs

Christopher L Sander (CL)

Department of Pharmacology, Case Western Reserve University, Cleveland, OH.
Department of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA.
ORCID: 0000-0002-1680-6637

Avery E Sears (AE)

Department of Pharmacology, Case Western Reserve University, Cleveland, OH.
Department of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA.
ORCID: 0000-0001-8878-6391

Antonio F M Pinto (AFM)

Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, CA.
ORCID: 0000-0002-1573-8011

Elliot H Choi (EH)

Department of Pharmacology, Case Western Reserve University, Cleveland, OH.
Department of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA.

Shirin Kahremany (S)

Department of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA.

Fangyuan Gao (F)

Department of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA.
ORCID: 0000-0002-1270-8781

David Salom (D)

Department of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA.
ORCID: 0000-0002-3208-1509

Hui Jin (H)

Department of Pharmacology, Case Western Reserve University, Cleveland, OH.

Els Pardon (E)

Vlaams Instituut voor Biotechnologie-Vrije Universiteit Brussel Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Brussels, Belgium.
Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.

Susie Suh (S)

Department of Pharmacology, Case Western Reserve University, Cleveland, OH.
Department of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA.

Zhiqian Dong (Z)

Department of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA.

Jan Steyaert (J)

Vlaams Instituut voor Biotechnologie-Vrije Universiteit Brussel Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Brussels, Belgium.
Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.

Alan Saghatelian (A)

Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, CA.
ORCID: 0000-0002-0427-563X

Dorota Skowronska-Krawczyk (D)

Department of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA.
Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA.
ORCID: 0000-0002-5758-4225

Philip D Kiser (PD)

Department of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA.
Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA.
Research Service, VA Long Beach Healthcare System, Long Beach, CA.
ORCID: 0000-0003-1184-9539

Krzysztof Palczewski (K)

Department of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA.
Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA.
Department of Chemistry, University of California, Irvine, Irvine, CA.
ORCID: 0000-0002-0788-545X

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Classifications MeSH

Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Transporteurs ABC › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Enzymes et coenzymes › Enzymes › Oxidoreductases › Alcohol oxidoreductases › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Eucaryotes › Animaux › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Bovins › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Cellules › Structures cellulaires › Membrane cellulaire › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines de l'oeil › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Disciplines des sciences naturelles › Chimie › Biochimie › Métabolomique › Lipidomique › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Lipides › Lipides membranaires › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée BALB C › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › Souris knockout › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Techniques d'investigation › Microscopie électronique › Microscopie électronique à transmission › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Technologie, industrie et agriculture › Technologie › Miniaturisation › Nanotechnologie › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Structures macromoléculaires › Périphérines › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Cellules › Neurones › Neurones afférents › Cellules réceptrices sensorielles › Cellules photoréceptrices › Cellules photoréceptrices de vertébré › Cellules photoréceptrices en bâtonnet de la rétine › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Facteurs biologiques › Pigments biologiques › Pigments rétiniens › Opsines › Opsines des bâtonnets › Rhodopsine › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Fragments d'immunoglobuline › Fragments Fab d'immunoglobuline › Anticorps à domaine unique › Nano-scale resolution of native retinal rod...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Tétraspanines › Nano-scale resolution of native retinal rod...