Platelet function and bleeding at different phases of childhood immune thrombocytopenia.

Adolescent Blood Platelets / drug effects Calcium Signaling Case-Control Studies Child Child, Preschool Female Hemorrhage / etiology Humans Infant Male Platelet Function Tests Purpura, Thrombocytopenic, Idiopathic / blood Receptors, Fc / therapeutic use Recombinant Fusion Proteins / pharmacology Thrombopoietin / pharmacology

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
30 04 2021
Historique:
received: 20 01 2021
accepted: 14 04 2021
entrez: 1 5 2021
pubmed: 2 5 2021
medline: 28 10 2021
Statut: epublish

Résumé

Immune thrombocytopenia (ITP) is believed to be associated with platelet function defects. However, their mechanisms are poorly understood, in particular with regard to differences between ITP phases, patient age, and therapy. We investigated platelet function and bleeding in children with either persistent or chronic ITP, with or without romiplostim therapy. The study included 151 children with ITP, of whom 56 had disease duration less than 12 months (grouped together as acute/persistent) and 95 were chronic. Samples of 57 healthy children were used as controls, while 5 patients with leukemia, 5 with aplastic anemia, 4 with MYH9-associated thrombocytopenia, and 7 with Wiskott-Aldrich syndrome were used as non-ITP thrombocytopenia controls. Whole blood flow cytometry revealed that platelets in both acute/persistent and chronic ITP were increased in size compared with healthy donors. They were also pre-activated as assessed by PAC1, CD62p, cytosolic calcium, and procoagulant platelet levels. This pattern was not observed in other childhood thrombocytopenias. Pre-activation by CD62p was higher in the bleeding group in the chronic ITP cohort only. Romiplostim treatment decreased size and pre-activation of the patient platelets, but not calcium. Our data suggest that increased size, pre-activation, and cytosolic calcium are common for all ITP platelets, but their association with bleeding could depend on the disease phase.

Identifiants

DOI: 10.1038/s41598-021-88900-6 PMID: 33931737 PMC: PMC8087794
pubmed: 33931737
doi: 10.1038/s41598-021-88900-6
pii: 10.1038/s41598-021-88900-6
pmc: PMC8087794
doi:

Substances chimiques

Receptors, Fc 0
Recombinant Fusion Proteins 0
Thrombopoietin 9014-42-0
romiplostim GN5XU2DXKV

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

9401

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Auteurs

Anastasia A Ignatova (AA)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.
Center for Theoretical Problems of Physicochemical Pharmacology of the Russian Academy of Sciences, Moscow, Russia.

Elena V Suntsova (EV)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Alexey V Pshonkin (AV)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Alexey A Martyanov (AA)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.
Center for Theoretical Problems of Physicochemical Pharmacology of the Russian Academy of Sciences, Moscow, Russia.
Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia.
Institute for Biochemical Physics (IBCP), Russian Academy of Sciences (RAS), Moscow, Russia.

Evgeniya A Ponomarenko (EA)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.
Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.

Dmitry M Polokhov (DM)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Daria V Fedorova (DV)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Kirill A Voronin (KA)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Natalia N Kotskaya (NN)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Natalia M Trubina (NM)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Marina V Krasilnikova (MV)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Selima Sh Uzueva (SS)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Irina V Serkova (IV)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Galina S Ovsyannikova (GS)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Ksenia I Romanova (KI)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Lili A Hachatryan (LA)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Irina I Kalinina (II)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Viktor E Matveev (VE)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Maya N Korsantiya (MN)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Natalia S Smetanina (NS)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Dmitry A Evseev (DA)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Maria N Sadovskaya (MN)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Kristina S Antonova (KS)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Anna L Khoreva (AL)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Pavel A Zharkov (PA)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Anna Shcherbina (A)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Anastasia N Sveshnikova (AN)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.
Center for Theoretical Problems of Physicochemical Pharmacology of the Russian Academy of Sciences, Moscow, Russia.
Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia.
Department of Normal Physiology, Sechenov First Moscow State Medical University, Moscow, Russia.

Aleksey A Maschan (AA)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Galina A Novichkova (GA)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997.

Mikhail A Panteleev (MA)

National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, 1 Samory Mashela Str, Moscow, Russia, 117997. mapanteleev@yandex.ru.
Center for Theoretical Problems of Physicochemical Pharmacology of the Russian Academy of Sciences, Moscow, Russia. mapanteleev@yandex.ru.
Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia. mapanteleev@yandex.ru.

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Classifications MeSH

Recherchemedicale.com › Personnes › Tranches d'âge › Adolescent › Platelet function and bleeding at different...
Recherchemedicale.com › Systèmes sanguin et immunitaire › Sang › Cellules sanguines › Plaquettes › Platelet function and bleeding at different...
Recherchemedicale.com › Phénomènes physiologiques cellulaires › Transduction du signal › Systèmes de seconds messagers › Signalisation calcique › Platelet function and bleeding at different...
Recherchemedicale.com › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études épidémiologiques › Études cas-témoins › Platelet function and bleeding at different...
Recherchemedicale.com › Personnes › Tranches d'âge › Enfant › Platelet function and bleeding at different...
Recherchemedicale.com › Personnes › Tranches d'âge › Enfant › Enfant d'âge préscolaire › Platelet function and bleeding at different...
Recherchemedicale.com › États, signes et symptômes pathologiques › Processus pathologiques › Hémorragie › Platelet function and bleeding at different...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Platelet function and bleeding at different...
Recherchemedicale.com › Personnes › Tranches d'âge › Nourrisson › Platelet function and bleeding at different...
Recherchemedicale.com › Techniques d'investigation › Techniques de laboratoire clinique › Tests hématologiques › Tests fonctionnels plaquettaires › Platelet function and bleeding at different...
Recherchemedicale.com › États, signes et symptômes pathologiques › Signes et symptômes › Manifestations cutanées › Purpura › Purpura thrombopénique › Purpura thrombopénique idiopathique › Platelet function and bleeding at different...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Récepteur Fc › Platelet function and bleeding at different...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Protéines de fusion recombinantes › Platelet function and bleeding at different...
Recherchemedicale.com › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Facteurs de croissance hématopoïétique › Facteurs de stimulation des colonies › Thrombopoïétine › Platelet function and bleeding at different...