Mass spectrometry imaging of free-floating brain sections detects pathological lipid distribution in a mouse model of Alzheimer's-like pathology.
Alzheimer Disease
/ metabolism
Amyloid beta-Protein Precursor
/ genetics
Animals
Astrocytes
/ metabolism
Cerebral Cortex
/ drug effects
Formaldehyde
/ chemistry
Hippocampus
/ drug effects
Lipid Metabolism
Lipids
/ analysis
Liraglutide
/ pharmacology
Male
Mice, Inbred C57BL
Mice, Transgenic
Plaque, Amyloid
/ metabolism
Polymers
/ chemistry
Presenilin-1
/ genetics
Prolactin-Releasing Hormone
/ analogs & derivatives
Specimen Handling
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Journal
The Analyst
ISSN: 1364-5528
Titre abrégé: Analyst
Pays: England
ID NLM: 0372652
Informations de publication
Date de publication:
07 Jul 2020
07 Jul 2020
Historique:
pubmed:
22
5
2020
medline:
16
4
2021
entrez:
22
5
2020
Statut:
ppublish
Résumé
Mass spectrometry imaging (MSI) is a modern analytical technique capable of monitoring the spatial distribution of compounds within target tissues. Collection and storage are important steps in sample preparation. The recommended and most widely used preservation procedure for MSI is freezing samples in isopentane and storing them at temperatures below -80 °C. On the other hand, the most common and general method for preserving biological samples in clinical practice is fixation in paraformaldehyde. Special types of samples prepared from these fixed tissues that are used for histology and immunohistochemistry are free-floating sections. It would be very beneficial if the latter procedure could also be applicable for the samples intended for subsequent MSI analysis. In the present work, we optimized and evaluated paraformaldehyde-fixed free-floating sections for the analysis of lipids in mouse brains and used the sections for the study of lipid changes in double transgenic APP/PS1 mice, a model of Alzheimer's-like pathology. Moreover, we examined the neuroprotective properties of palm
Substances chimiques
APP protein, human
0
Amyloid beta-Protein Precursor
0
Lipids
0
PSEN1 protein, human
0
Polymers
0
Presenilin-1
0
Prolactin-Releasing Hormone
0
palm11-PrRP31
0
Formaldehyde
1HG84L3525
Liraglutide
839I73S42A
paraform
Y19UC83H8E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM