Zygophyllum album leaves extract prevented hepatic fibrosis in rats, by reducing liver injury and suppressing oxidative stress, inflammation, apoptosis and the TGF-β1/Smads signaling pathways. Exploring of bioactive compounds using HPLC-DAD-ESI-QTOF-MS/MS.
Animals
Anti-Inflammatory Agents
/ pharmacology
Antioxidants
/ pharmacology
Apoptosis
/ drug effects
Chromatography, High Pressure Liquid
/ methods
Cytokines
/ metabolism
Hepatic Stellate Cells
/ drug effects
Inflammation
/ drug therapy
Liver
/ drug effects
Liver Cirrhosis
/ drug therapy
Male
Oxidative Stress
/ drug effects
Plant Extracts
/ pharmacology
Plant Leaves
/ chemistry
Protective Agents
/ pharmacology
Rats
Rats, Wistar
Signal Transduction
/ drug effects
Smad Proteins
/ metabolism
Tandem Mass Spectrometry
/ methods
Transforming Growth Factor beta1
/ metabolism
Zygophyllum
/ chemistry
Apoptosis
Fibrosis
HPLC–DAD–ESI–QTOF-MS
Inflammation
Oxidative stress
Zygophyllum album
Journal
Inflammopharmacology
ISSN: 1568-5608
Titre abrégé: Inflammopharmacology
Pays: Switzerland
ID NLM: 9112626
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
09
12
2019
accepted:
10
03
2020
pubmed:
25
3
2020
medline:
14
7
2021
entrez:
25
3
2020
Statut:
ppublish
Résumé
Zygophyllum album is traditionally used against many illnesses, such as liver disease. The present study investigated the bioactive compounds in methanol extract of Z. album (MEZA) using HPLC-DAD-ESI-QTOF-MS/MS and explored its possible antioxidative, anti-inflammatory, anti-apoptotic, and hepatoprotective effect. Twelve phenolic compounds were identified; isorhamnetin-3-O-rutinoside being the main one was the main composite (144.6 mg/100 g dm). Results showed that MEZA reduced significantly the biochemical markers (AST, ALT, LDH and ALP), and the hepatic oxidative stress indicators (MDA, PC, SOD, CAT, and GPx) in deltamethrin (DLM)-treated rats. Moreover, MEZA limited the inflammatory responses through downregulation of NF-κB gene, which suppressed the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6). Furthermore, Z. album reduced DLM-induced apoptosis by attenuating caspase 3 and p53 mRNA activation. MEZA treatment also alleviated upregulation of α-SMA, type I collagen, and TGF-β1 mRNA in the liver. The possible antifibrotic effect of MEZA was clearly demonstrated by the histopathology examination, using Masson's Trichrome and Sirius Red stainings. Therefore, the current study suggested that the bioactive compounds of Z. album possessed antifibrotic effect against DLM-induced hepatic fibrosis, by protecting liver tissue, and inhibiting oxidative stress, inflammation, apoptosis and the TGF-β1/Smads signaling pathways.
Identifiants
pubmed: 32206981
doi: 10.1007/s10787-020-00700-y
pii: 10.1007/s10787-020-00700-y
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Antioxidants
0
Cytokines
0
Plant Extracts
0
Protective Agents
0
Smad Proteins
0
Transforming Growth Factor beta1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM