Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling.
Animals
Cattle
Chemokines
/ genetics
Cytokines
/ genetics
Endometritis
/ drug therapy
Epithelial Cells
/ drug effects
Female
Inflammation
/ prevention & control
Iridoid Glucosides
/ pharmacology
Lipopolysaccharides
/ pharmacology
Mice
NF-kappa B
/ physiology
Signal Transduction
/ drug effects
Toll-Like Receptor 4
/ physiology
Catalpol; Endometritis; Inflammation; Toll-like receptor 4 (TLR4); Nuclear factor-κB (NF-κB)
Journal
Journal of Zhejiang University. Science. B
ISSN: 1862-1783
Titre abrégé: J Zhejiang Univ Sci B
Pays: China
ID NLM: 101236535
Informations de publication
Date de publication:
Historique:
entrez:
7
9
2019
pubmed:
7
9
2019
medline:
20
2
2020
Statut:
ppublish
Résumé
Catalpol is the main active ingredient of an extract from Radix rehmanniae, which in a previous study showed a protective effect against various types of tissue injury. However, a protective effect of catalpol on uterine inflammation has not been reported. In this study, to investigate the protective mechanism of catalpol on lipopolysaccharide (LPS)-induced bovine endometrial epithelial cells (bEECs) and mouse endometritis, in vitro and in vivo inflammation models were established. The Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway and its downstream inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR), western blot (WB), and immunofluorescence techniques. The results from ELISA and qRT-PCR showed that catalpol dose-dependently reduced the expression of pro-inflammatory cytokines such as tumor necrosis factor α (TNF-α), interleukin (IL)-1β, and IL-6, and chemokines such as C-X-C motif chemokine ligand 8 (CXCL8) and CXCL5, both in bEECs and in uterine tissue. From the experimental results of WB, qRT-PCR, and immunofluorescence, the expression of TLR4 and the phosphorylation of NF-κB p65 were markedly inhibited by catalpol compared with the LPS group. The inflammatory damage to the mouse uterus caused by LPS was greatly reduced and was accompanied by a decline in myeloperoxidase (MPO) activity. The results of this study suggest that catalpol can exert an anti-inflammatory impact on LPS-induced bEECs and mouse endometritis by inhibiting inflammation and activation of the TLR4/NF-κB signaling pathway.
Identifiants
pubmed: 31489801
doi: 10.1631/jzus.B1900071
pmc: PMC6751487
doi:
Substances chimiques
Chemokines
0
Cytokines
0
Iridoid Glucosides
0
Lipopolysaccharides
0
NF-kappa B
0
Toll-Like Receptor 4
0
catalpol
2415-24-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
816-827Commentaires et corrections
Type : ErratumIn
Références
Clin Microbiol Rev. 2001 Oct;14(4):821-35, table of contents
pubmed: 11585787
Nat Rev Immunol. 2002 Oct;2(10):725-34
pubmed: 12360211
Brain Res. 2006 Dec 6;1123(1):68-79
pubmed: 17078935
J Immunol. 2007 Nov 15;179(10):7121-7
pubmed: 17982104
Neoplasia. 2008 Mar;10(3):244-54
pubmed: 18320069
Vet J. 2008 Apr;176(1):115-21
pubmed: 18329302
Exp Toxicol Pathol. 2009 Sep;61(5):461-9
pubmed: 19081713
Reprod Biol Endocrinol. 2009 May 29;7:55
pubmed: 19476661
Neurosci Lett. 2010 Apr 12;473(3):224-8
pubmed: 20219628
Cold Spring Harb Perspect Biol. 2010 Mar;2(3):a000158
pubmed: 20300203
BMC Microbiol. 2010 Jun 14;10:172
pubmed: 20546607
Eur J Cancer. 2012 Sep;48(14):2244-51
pubmed: 22197219
Pharm Biol. 2013 Apr;51(4):463-73
pubmed: 23336403
Endocrinology. 2014 Apr;155(4):1453-65
pubmed: 24437488
Clin Investig Arterioscler. 2014 Jul-Aug;26(4):184-92
pubmed: 24866730
Theriogenology. 2015 Jul 1;84(1):34-42
pubmed: 25765298
Theriogenology. 2015 Jul 1;84(1):11-8
pubmed: 25765299
J Vet Med Sci. 2016 Mar;78(3):375-81
pubmed: 26522810
Inflammation. 2016 Jun;39(3):1141-50
pubmed: 27052630
J Interferon Cytokine Res. 2017 Feb;37(2):81-89
pubmed: 27845845
Theriogenology. 2017 May;94:21-30
pubmed: 28407857
J Zhejiang Univ Sci B. 2017 Jun;18(6):481-491
pubmed: 28585424
Prostaglandins Leukot Essent Fatty Acids. 2018 Feb;129:25-31
pubmed: 29482767
Theriogenology. 2018 Oct 1;119:225-232
pubmed: 30055393
Vet Res. 2018 Aug 2;49(1):77
pubmed: 30068391
J Zhejiang Univ Sci B. 2018 Aug.;19(8):654-661
pubmed: 30070088
Gene. 2018 Nov 30;677:266-272
pubmed: 30077008
Theriogenology. 2018 Oct 15;120:117-122
pubmed: 30114545
Anim Reprod Sci. 2018 Oct;197:268-277
pubmed: 30195943
Front Pharmacol. 2018 Aug 30;9:982
pubmed: 30214410
Biochem Biophys Res Commun. 2018 Oct 20;505(1):1-6
pubmed: 30224056
Theriogenology. 2018 Dec;122:74-83
pubmed: 30243137
Biomed Pharmacother. 2018 Nov;107:1496-1504
pubmed: 30257367
Inflammation. 2019 Apr;42(2):650-657
pubmed: 30406463
J Zhejiang Univ Sci B. 2018 Dec.;19(12):910-923
pubmed: 30507075
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2532-6
pubmed: 8460169