Deep sequencing of small non-coding RNA highlights brain-specific expression patterns and RNA cleavage.


Journal

RNA biology
ISSN: 1555-8584
Titre abrégé: RNA Biol
Pays: United States
ID NLM: 101235328

Informations de publication

Date de publication:
12 2019
Historique:
pubmed: 23 8 2019
medline: 1 5 2020
entrez: 22 8 2019
Statut: ppublish

Résumé

With the advance of high-throughput sequencing technology numerous new regulatory small RNAs have been identified, that broaden the variety of processing mechanisms and functions of non-coding RNA. Here we explore small non-coding RNA (sncRNA) expression in central parts of the physiological stress and anxiety response system. Therefore, we characterize the sncRNA profile of tissue samples from Amygdala, Hippocampus, Hypothalamus and Adrenal Gland, obtained from 20 pigs. Our analysis reveals that all tissues but Amygdala and Hippocampus possess distinct, tissue-specific expression pattern of miRNA that are associated with Hypoxia, stress responses as well as memory and fear conditioning. In particular, we observe marked differences in the expression profile of limbic tissues compared to those associated to the HPA/stress axis, with a surprisingly high aggregation of 3´-tRNA halves in Amygdala and Hippocampus. Since regulation of sncRNA and RNA cleavage plays a pivotal role in the central nervous system, our work provides seminal insights in the role/involvement of sncRNA in the transcriptional and post-transcriptional regulation of negative emotion, stress and coping behaviour in pigs, and mammals in general.

Identifiants

pubmed: 31432767
doi: 10.1080/15476286.2019.1657743
pmc: PMC6844574
doi:

Substances chimiques

RNA, Small Untranslated 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1764-1774

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Auteurs

Fiete Haack (F)

Institute for Genome Biology, Functional Genome Analysis Research Unit, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.

Nares Trakooljul (N)

Institute for Genome Biology, Genomics Research Unit, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.

Kevin Gley (K)

Institute for Genome Biology, Functional Genome Analysis Research Unit, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.

Eduard Murani (E)

Institute for Genome Biology, Genomics Research Unit, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.

Frieder Hadlich (F)

Institute for Genome Biology, Functional Genome Analysis Research Unit, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.

Klaus Wimmers (K)

Institute for Genome Biology, Genomics Research Unit, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.
Faculty of Agricultural and Environmental Sciences, University Rostock, Rostock, Germany.

Siriluck Ponsuksili (S)

Institute for Genome Biology, Functional Genome Analysis Research Unit, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.

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Classifications MeSH