CRISPR-Cas bioinformatics.

Algorithms CRISPR-Cas Systems / genetics Computational Biology / methods Gene Editing RNA, Guide, Kinetoplastida / genetics
Anti-CRISPR proteins Bioinformatics CRISPR array CRISPR leader CRISPR-Cas Cas genes Direct repeats PAM Spacers crRNAs

Journal

Methods (San Diego, Calif.)
ISSN: 1095-9130
Titre abrégé: Methods
Pays: United States
ID NLM: 9426302

Informations de publication

Date de publication:
01 02 2020
Historique:
received: 01 03 2019
revised: 19 06 2019
accepted: 15 07 2019
pubmed: 22 7 2019
medline: 22 12 2020
entrez: 22 7 2019
Statut: ppublish

Résumé

Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated proteins (Cas) are essential genetic elements in many archaeal and bacterial genomes, playing a key role in a prokaryote adaptive immune system against invasive foreign elements. In recent years, the CRISPR-Cas system has also been engineered to facilitate target gene editing in eukaryotic genomes. Bioinformatics played an essential role in the detection and analysis of CRISPR systems and here we review the bioinformatics-based efforts that pushed the field of CRISPR-Cas research further. We discuss the bioinformatics tools that have been published over the last few years and, finally, present the most popular tools for the design of CRISPR-Cas9 guides.

Identifiants

DOI: 10.1016/j.ymeth.2019.07.013 PMID: 31326596
pubmed: 31326596
pii: S1046-2023(18)30471-7
doi: 10.1016/j.ymeth.2019.07.013
pii:
doi:

Substances chimiques

RNA, Guide 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

3-11

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Omer S Alkhnbashi (OS)

Chair of Bioinformatics, University of Freiburg, Freiburg, Germany. Electronic address: alkhanbo@informatik.uni-freiburg.de.

Tobias Meier (T)

Computational Biology, Institute of Biochemical Engineering, University of Stuttgart, Stuttgart, Germany. Electronic address: tobias.meier@ibvt.uni-stuttgart.de.

Alexander Mitrofanov (A)

Chair of Bioinformatics, University of Freiburg, Freiburg, Germany. Electronic address: mitrofan@informatik.uni-freiburg.de.

Rolf Backofen (R)

Chair of Bioinformatics, University of Freiburg, Freiburg, Germany; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Germany. Electronic address: backofen@informatik.uni-freiburg.de.

Björn Voß (B)

Computational Biology, Institute of Biochemical Engineering, University of Stuttgart, Stuttgart, Germany. Electronic address: bjoern.voss@ibvt.uni-stuttgart.de.

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Classifications MeSH

Recherchemedicale.com › Sciences de l'information › Méthodologies informatiques › Algorithmes › CRISPR-Cas bioinformatics.
Recherchemedicale.com › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › CRISPR-Cas bioinformatics.
Recherchemedicale.com › Sciences de l'information › Informatique › Biologie informatique › CRISPR-Cas bioinformatics.
Recherchemedicale.com › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › CRISPR-Cas bioinformatics.
Recherchemedicale.com › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › ARN guide › CRISPR-Cas bioinformatics.