Kunichika Tsumoto

Affiliations
  • Department of Physiology II, Kanazawa Medical University, 1-1 Daigaku, Uchinada, 920-0293, Japan. tsumoto@kanazawa-med.ac.jp.
  • Department of Pharmacology, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, 565-0871, Japan. tsumoto@kanazawa-med.ac.jp.
ORCID: 0000-0001-7457-3125

Publications (79)

Anion solvation enhanced by positive supercharging mutations preserves thermal stability of an antibody in a wide pH range.

Biochemical and biophysical research communications
Avec: Keisuke Kasahara , Daisuke Kuroda , Aki Tanabe , Raiji Kawade , Satoru Nagatoishi , Kouhei Tsumoto

A new twist of rubredoxin function in M. tuberculosis.

Bioorganic chemistry
Avec: Tatsiana Sushko , Anton Kavaleuski , Irina Grabovec , Anna Kavaleuskaya , Daniil Vakhrameev , Sergey Bukhdruker , Egor Marin , Alexey Kuzikov , Rami Masamrekh , Victoria Shumyantseva , Kouhei Tsumoto , Valentin Borshchevskiy , Andrei Gilep , Natallia Strushkevich

Specific decreasing of Na

Scientific reports
Avec: Takashi Ashihara , Narumi Naito , Takao Shimamoto , Akira Amano , Yasutaka Kurata , Yoshihisa Kurachi

Structural Basis for the Binding Mechanism of Human Serum Albumin Complexed with Cyclic Peptide Dalbavancin.

Journal of medicinal chemistry
Avec: Sho Ito , Akinobu Senoo , Satoru Nagatoishi , Masahito Ohue , Masaki Yamamoto , Kouhei Tsumoto , Naoki Wakui

Development of biparatopic bispecific antibody possessing tetravalent scFv-Fc capable of binding to ROBO1 expressed in hepatocellular carcinoma cells.

Journal of biochemistry
Avec: Yuji Watanabe , Aki Tanabe , Takao Hamakubo , Satoru Nagatoishi , Kouhei Tsumoto

Apoptotic Cell-Inspired Polymeric Particles for Controlling Microglial Inflammation toward Neurodegenerative Disease Treatment.

ACS biomaterials science & engineering
Avec: Yasuhiro Nakagawa , Yuto Yano , Jeonggyu Lee , Yasutaka Anraku , Makoto Nakakido , Kouhei Tsumoto , Horacio Cabral , Mitsuhiro Ebara

Technical Capabilities and Limitations of Optical Spectroscopy and Calorimetry Using Water-Miscible Solvents: The Case of Dimethyl Sulfoxide, Acetonitrile, and 1,4-Dioxane.

Journal of pharmaceutical sciences
Avec: Atsushi Hirano , Satoru Nagatoishi , Momoyo Wada , Kouhei Tsumoto , Karl N Maluf , Tsutomu Arakawa

A Peptoid with Extended Shape in Water.

Journal of the American Chemical Society
Avec: Jumpei Morimoto , Yasuhiro Fukuda , Daisuke Kuroda , Takumu Watanabe , Fumihiko Yoshida , Mizue Asada , Toshikazu Nakamura , Akinobu Senoo , Satoru Nagatoishi , Kouhei Tsumoto , Shinsuke Sando

An epitope-directed antibody affinity maturation system utilizing mammalian cell survival as readout.

Biotechnology and bioengineering
Avec: Akihiro Eguchi , Makoto Nakakido , Satoru Nagatoishi , Daisuke Kuroda , Kouhei Tsumoto , Teruyuki Nagamune , Masahiro Kawahara

Membrane fusion and infection abilities of baculovirus virions are preserved during freezing and thawing in the presence of trehalose.

Bioscience, biotechnology, and biochemistry
Avec: Kohei Nakanishi , Masahiro Tomita , Kanta Tsumoto

The Kg-antigen, RhAG with a Lys164Gln mutation, gives rise to haemolytic disease of the newborn.

British journal of haematology
Avec: Mitsunobu Tanaka , Takaaki Abe , Takeharu Minamitani , Hiroki Akiba , Toshihiro Horikawa , Ryutaro Tobita , Kazumi Isa , Kenichi Ogasawara , Hideo Takahashi , Hidemi Tateyama , Satomi Tone , Kouhei Tsumoto , Teruhito Yasui , Takafumi Kimura , Yoshihiro Fujimura , Fumiya Hirayama , Yoshihiko Tani , Yoshihiro Takihara

Generation of biparatopic antibody through two-step targeting of fragment antibodies on antigen using SpyTag and SpyCatcher.

Biotechnology reports (Amsterdam, Netherlands)
Avec: Hiroki Akiba , Kensuke Takayanagi , Osamu Kusano-Arai , Hiroko Iwanari , Takao Hamakubo , Kouhei Tsumoto

System-Wide Analysis of Protein Acetylation and Ubiquitination Reveals a Diversified Regulation in Human Cancer Cells.

Biomolecules
Avec: Hiroko Kozuka-Hata , Aya Kitamura , Tomoko Hiroki , Aiko Aizawa , Kouhei Tsumoto , Jun-Ichiro Inoue , Masaaki Oyama

Highly sensitive HPLC analysis and biophysical characterization of N-glycans of IgG-Fc domain in comparison between CHO and 293 cells using FcγRIIIa ligand.

Biotechnology progress
Avec: Hirofumi Kosuge , Satoru Nagatoishi , Masato Kiyoshi , Akiko Ishii-Watabe , Toru Tanaka , Yosuke Terao , Seigo Oe , Teruhiko Ide , Kouhei Tsumoto

Optimization of stereospecific targeting technique for selective production of monoclonal antibodies against native ephrin type-A receptor 2.

Journal of immunological methods
Avec: Yasuhiro Yamasaki , Chiho Miyamae , Yushi Isozaki , Keisuke Ichikawa , Yoshiki Kaneko , Yasuyuki Oda , Takashi Murayama , Takashi Sakurai , Katsuyuki Tamai , Kanta Tsumoto , Masahiro Tomita

Microsecond molecular dynamics suggest that a non-synonymous mutation, frequently observed in patients with mild symptoms in Tokyo, alters dynamics of the SARS-CoV-2 main protease.

Biophysics and physicobiology
Avec: Daisuke Kuroda , Kouhei Tsumoto

Structure and role of the linker domain of the iron surface-determinant protein IsdH in heme transportation in Staphylococcus aureus.

The Journal of biological chemistry
Avec: Sandra Valenciano-Bellido , Jose M M Caaveiro , Koldo Morante , Tatyana Sushko , Makoto Nakakido , Satoru Nagatoishi , Kouhei Tsumoto

Structural basis for the recognition of human hemoglobin by the heme-acquisition protein Shr from Streptococcus pyogenes.

Scientific reports
Avec: Akinobu Senoo , Masato Hoshino , Toshiki Shiomi , Makoto Nakakido , Satoru Nagatoishi , Daisuke Kuroda , Ichiro Nakagawa , Jeremy R H Tame , Jose M M Caaveiro , Kouhei Tsumoto

Cryo-EM structures elucidate the multiligand receptor nature of megalin.

Proceedings of the National Academy of Sciences of the United States of America
Avec: Sawako Goto , Akihisa Tsutsumi , Yongchan Lee , Michihiro Hosojima , Hideyuki Kabasawa , Koichi Komochi , Satoru Nagatoishi , Kazuya Takemoto , Kouhei Tsumoto , Tomohiro Nishizawa , Masahide Kikkawa , Akihiko Saito

Human antibody recognition and neutralization mode on the NTD and RBD domains of SARS-CoV-2 spike protein.

Scientific reports
Avec: Ryota Otsubo , Takeharu Minamitani , Kouji Kobiyama , Junso Fujita , Toshihiro Ito , Shiori Ueno , Itsuki Anzai , Hiroki Tanino , Hiroshi Aoyama , Yoshiharu Matsuura , Keiichi Namba , Ken-Ichi Imadome , Ken J Ishii , Kouhei Tsumoto , Wataru Kamitani , Teruhito Yasui

Advanced hybridoma technology for selective production of high-affinity monoclonal antibodies through B-cell receptors.

Journal of immunological methods
Avec: Kento Sakashita , Kanta Tsumoto , Masahiro Tomita

Repression of the PRELP gene is relieved by histone deacetylase inhibitors through acetylation of histone H2B lysine 5 in bladder cancer.

Clinical epigenetics
Avec: Kanto Shozu , Syuzo Kaneko , Norio Shinkai , Ai Dozen , Hirofumi Kosuge , Makoto Nakakido , Hidenori Machino , Ken Takasawa , Ken Asada , Masaaki Komatsu , Kouhei Tsumoto , Shin-Ichi Ohnuma , Ryuji Hamamoto

Peripheral administration of nanomicelle-encapsulated anti-Aβ oligomer fragment antibody reduces various toxic Aβ species in the brain.

Journal of nanobiotechnology
Avec: Akiko Amano , Nobuo Sanjo , Wataru Araki , Yasutaka Anraku , Makoto Nakakido , Etsuro Matsubara , Takami Tomiyama , Tetsuya Nagata , Kouhei Tsumoto , Kazunori Kataoka , Takanori Yokota

Structural insights into 3Fe-4S ferredoxins diversity in

Frontiers in molecular biosciences
Avec: Andrei Gilep , Tatsiana Varaksa , Sergey Bukhdruker , Anton Kavaleuski , Yury Ryzhykau , Sviatlana Smolskaya , Tatsiana Sushko , Kouhei Tsumoto , Irina Grabovec , Ivan Kapranov , Ivan Okhrimenko , Egor Marin , Mikhail Shevtsov , Alexey Mishin , Kirill Kovalev , Alexander Kuklin , Valentin Gordeliy , Leonid Kaluzhskiy , Oksana Gnedenko , Evgeniy Yablokov , Alexis Ivanov , Valentin Borshchevskiy , Natallia Strushkevich

Elucidating Conformational Dynamics and Thermostability of Designed Aromatic Clusters by Using Protein Cages.

Chemistry (Weinheim an der Bergstrasse, Germany)
Avec: Yuki Hishikawa , Hiroki Noya , Satoru Nagatoishi , Takashi Yoshidome , Basudev Maity , Kouhei Tsumoto , Satoshi Abe , Takafumi Ueno

Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation.

Communications chemistry
Avec: Hiroki Sakuta , Naoki Nakatani , Takayuki Torisawa , Yutaka Sumino , Kanta Tsumoto , Kazuhiro Oiwa , Kenichi Yoshikawa

Non-Affinity Purification of Antibodies.

Antibodies (Basel, Switzerland)
Avec: Tsutomu Arakawa , Yui Tomioka , Masataka Nakagawa , Chiaki Sakuma , Yasunori Kurosawa , Daisuke Ejima , Kouhei Tsumoto , Teruo Akuta

Design of single-domain VHH antibodies to increase the binding activity in SPR amine coupling.

Biochemical and biophysical research communications
Avec: Atsunori Hirao , Satoru Nagatoishi , Emina Ikeuchi , Tsukushi Yamawaki , Chinatsu Mori , Makoto Nakakido , Kouhei Tsumoto

Raman Spectroscopic Analysis of Highly-Concentrated Antibodies under the Acid-Treated Conditions.

Pharmaceutical research
Avec: Yusui Sato , Satoru Nagatoishi , Shintaro Noguchi , Kouhei Tsumoto

Modulation of a conformational ensemble by a small molecule that inhibits key protein-protein interactions involved in cell adhesion.

Protein science : a publication of the Protein Society
Avec: Akinobu Senoo , Satoru Nagatoishi , Daisuke Kuroda , Sho Ito , Go Ueno , Jose M M Caaveiro , Kouhei Tsumoto

Arginine cluster introduction on framework region in anti-lysozyme antibody improved association rate constant by changing conformational diversity of CDR loops.

Protein science : a publication of the Protein Society
Avec: Shingo Maeta , Makoto Nakakido , Hiroaki Matsuura , Naoki Sakai , Kunio Hirata , Daisuke Kuroda , Atsushi Fukunaga , Kouhei Tsumoto

Specific peptide conjugation to a therapeutic antibody leads to enhanced therapeutic potency and thermal stability by reduced Fc dynamics.

Scientific reports
Avec: Masato Kiyoshi , Makoto Nakakido , Abdur Rafique , Minoru Tada , Michihiko Aoyama , Yosuke Terao , Satoru Nagatoishi , Hiroko Shibata , Teruhiko Ide , Kouhei Tsumoto , Yuji Ito , Akiko Ishii-Watabe

Quantitative analysis of antibody aggregates by combination of pinched-flow fractionation and coulter method.

Analytical biochemistry
Avec: Satoru Nagatoishi , Toshinobu Toyoshima , Kotohiro Furukawa , Kouhei Tsumoto

64Cu-labeled minibody D2101 visualizes CDH17-positive gastric cancer xenografts with short waiting time.

Nuclear medicine communications
Avec: Kentaro Fujiwara , Hiroki Akiba , Atsushi B Tsuji , Hitomi Sudo , Aya Sugyo , Kotaro Nagatsu , Ming-Rong Zhang , Hiroko Iwanari , Osamu Kusano-Arai , Shota Kudo , Chika Kikuchi , Kouhei Tsumoto , Toshimitsu Momose , Takao Hamakubo , Tatsuya Higashi

Self-Emergent Protocells Generated in an Aqueous Solution with Binary Macromolecules through Liquid-Liquid Phase Separation.

Chembiochem : a European journal of chemical biology
Avec: Hiroki Sakuta , Fumika Fujita , Tsutomu Hamada , Masahito Hayashi , Kingo Takiguchi , Kanta Tsumoto , Kenichi Yoshikawa

Effects of a remote mutation from the contact paratope on the structure of CDR-H3 in the anti-HIV neutralizing antibody PG16.

Scientific reports
Avec: Hiroko X Kondo , Ryo Kiribayashi , Daisuke Kuroda , Jiro Kohda , Akimitsu Kugimiya , Yasuhisa Nakano , Kouhei Tsumoto , Yu Takano

Biophysical characterization of the breast cancer-related BIG3-PHB2 interaction: Effect of non-conserved loop region of BIG3 on the structure and the interaction.

Biochemical and biophysical research communications
Avec: Takeru Chigira , Satoru Nagatoishi , Hiroyuki Takeda , Tetsuro Yoshimaru , Toyomasa Katagiri , Kouhei Tsumoto

Future perspectives of therapeutic monoclonal antibodies.

Immunotherapy
Avec: Kanta Tsumoto , Yushi Isozaki , Hisanori Yagami , Masahiro Tomita

Structural and thermodynamic basis for the recognition of the substrate-binding cleft on hen egg lysozyme by a single-domain antibody.

Scientific reports
Avec: Hiroki Akiba , Hiroko Tamura , Masato Kiyoshi , Saeko Yanaka , Kenji Sugase , Jose M M Caaveiro , Kouhei Tsumoto

Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device support.

Scientific reports
Avec: Toshihiro Ito , Takeharu Minamitani , Masaki Hayakawa , Ryota Otsubo , Hiroki Akiba , Kouhei Tsumoto , Masanori Matsumoto , Teruhito Yasui

Proteomic identification and validation of novel interactions of the putative tumor suppressor PRELP with membrane proteins including IGFI-R and p75NTR.

The Journal of biological chemistry
Avec: Hirofumi Kosuge , Makoto Nakakido , Satoru Nagatoishi , Tetsuya Fukuda , Yasuhiko Bando , Shin-Ichi Ohnuma , Kouhei Tsumoto

Elaboration of Non-naturally Occurring Helical Tripeptides as p53-MDM2/MDMX Interaction Inhibitors.

Chemical & pharmaceutical bulletin
Avec: Aoze Su , Yuko Tabata , Kiyono Aoki , Akane Sada , Rieko Ohki , Satoru Nagatoishi , Kouhei Tsumoto , Siyuan Wang , Yuko Otani , Tomohiko Ohwada

Molecular basis for the activation of actinoporins by lipids.

Methods in enzymology
Avec: Jose M M Caaveiro , Kouhei Tsumoto

An integrated computational pipeline for designing high-affinity nanobodies with expanded genetic codes.

Briefings in bioinformatics
Avec: Aditya K Padhi , Ashutosh Kumar , Ken-Ichi Haruna , Haruna Sato , Hiroko Tamura , Satoru Nagatoishi , Kouhei Tsumoto , Atushi Yamaguchi , Fumie Iraha , Mihoko Takahashi , Kensaku Sakamoto , Kam Y J Zhang

Improvement of the affinity of an anti-rat P2X4 receptor antibody by introducing electrostatic interactions.

Scientific reports
Avec: Chinatsu Shinozaki , Keita Kohno , Mitsunori Shiroishi , Daisuke Takahashi , Yu Yoshikawa , Yoshito Abe , Kenji Hamase , Makoto Nakakido , Kohei Tsumoto , Kazuhide Inoue , Makoto Tsuda , Tadashi Ueda

Discovery of a cystathionine γ-lyase (CSE) selective inhibitor targeting active-site pyridoxal 5'-phosphate (PLP) via Schiff base formation.

Scientific reports
Avec: Honami Echizen , Kenjiro Hanaoka , Kazuhito Shimamoto , Ryota Hibi , Sachiko Toma-Fukai , Hisashi Ohno , Eita Sasaki , Toru Komatsu , Tasuku Ueno , Yukihiro Tsuchiya , Yasuo Watanabe , Takao Otsuka , Hiroaki Saito , Satoru Nagatoishi , Kouhei Tsumoto , Hirotatsu Kojima , Takayoshi Okabe , Toshiyuki Shimizu , Yasuteru Urano

Development of a 1:1-binding biparatopic anti-TNFR2 antagonist by reducing signaling activity through epitope selection.

Communications biology
Avec: Hiroki Akiba , Junso Fujita , Tomoko Ise , Kentaro Nishiyama , Tomoko Miyata , Takayuki Kato , Keiichi Namba , Hiroaki Ohno , Haruhiko Kamada , Satoshi Nagata , Kouhei Tsumoto

Conformational features and interaction mechanisms of V

Protein science : a publication of the Protein Society
Avec: Koichi Yamamoto , Satoru Nagatoishi , Ryo Matsunaga , Makoto Nakakido , Daisuke Kuroda , Kouhei Tsumoto

Ladder observation of bovine serum albumin by high resolution agarose native gel electrophoresis.

International journal of biological macromolecules
Avec: Yui Tomioka , Masataka Nakagawa , Chiaki Sakuma , Satoru Nagatoishi , Kouhei Tsumoto , Tsutomu Arakawa , Teruo Akuta

Analytical Method for Experimental Validation of Computer-Designed Antibody.

Methods in molecular biology (Clifton, N.J.)
Avec: Aki Tanabe , Kouhei Tsumoto

Cancers
Avec: Jack Hopkins , Ken Asada , Alex Leung , Vasiliki Papadaki , Hongorzul Davaapil , Matthew Morrison , Tomoko Orita , Ryohei Sekido , Hirofumi Kosuge , M Ashwin Reddy , Kazuhiro Kimura , Akihisa Mitani , Kouhei Tsumoto , Ryuji Hamamoto , Mandeep S Sagoo , Shin-Ichi Ohnuma

Ligand Installation to Polymeric Micelles for Pediatric Brain Tumor Targeting.

Polymers
Avec: Takayoshi Watanabe , Hayato Laurence Mizuno , Jumpei Norimatsu , Takumi Obara , Horacio Cabral , Kouhei Tsumoto , Makoto Nakakido , Daisuke Kawauchi , Yasutaka Anraku

In vitro validation of chromogenic substrate assay for evaluation of surrogate FVIII-activity of emicizumab.

Thrombosis research
Avec: Tomoko Yamaguchi , Keiko Shinozawa , Satoru Nagatoishi , Ayano Mitsuhashi , Masato Bingo , Hiroshi Inaba , Kagehiro Amano , Kohei Tsumoto , Ei Kinai

Membranous and Membraneless Interfaces-Origins of Artificial Cellular Complexity.

Life (Basel, Switzerland)
Avec: Pasquale Stano , Kanta Tsumoto

Group A Streptococcus cation diffusion facilitator proteins contribute to immune evasion by regulating intracellular metal concentrations.

Biochemical and biophysical research communications
Avec: Chihiro Aikawa , Akihide Shimizu , Makoto Nakakido , Kazunori Murase , Takashi Nozawa , Kouhei Tsumoto , Ichiro Nakagawa

THOUSAND-GRAIN WEIGHT 6, which is an IAA-glucose hydrolase, preferentially recognizes the structure of the indole ring.

Scientific reports
Avec: Tatsuki Akabane , Nobuhiro Suzuki , Kazuyoshi Ikeda , Tomoki Yonezawa , Satoru Nagatoishi , Hiroyoshi Matsumura , Takuya Yoshizawa , Wataru Tsuchiya , Satoshi Kamino , Kouhei Tsumoto , Ken Ishimaru , Etsuko Katoh , Naoki Hirotsu

Thermodynamic and molecular dynamic insights into how fusion influences peptide-tag recognition of an antibody.

Scientific reports
Avec: Kazuhiro Miyanabe , Takefumi Yamashita , Kouhei Tsumoto

Impact of single-residue mutations on protein thermal stability: The case of threonine 83 of BC2L-CN lectin.

International journal of biological macromolecules
Avec: Megumi Hoya , Ryo Matsunaga , Satoru Nagatoishi , Teruhiko Ide , Daisuke Kuroda , Kouhei Tsumoto

Crystal structures of human CD40 in complex with monoclonal antibodies dacetuzumab and bleselumab.

Biochemical and biophysical research communications
Avec: Risa Asano , Makoto Nakakido , Jorge Fernández Pérez , Tomoko Ise , Jose M M Caaveiro , Satoshi Nagata , Kouhei Tsumoto

Development of novel humanized VHH synthetic libraries based on physicochemical analyses.

Scientific reports
Avec: Makoto Nakakido , Seisho Kinoshita , Kouhei Tsumoto

Structural basis for the ligand promiscuity of the hydroxamate siderophore binding protein FtsB from Streptococcus pyogenes.

Structure (London, England : 1993)
Avec: Jorge Fernandez-Perez , Akinobu Senoo , Jose M M Caaveiro , Makoto Nakakido , Susana de Vega , Ichiro Nakagawa , Kouhei Tsumoto

Multiple Dynamical Mechanisms of Phase-2 Early Afterdepolarizations in a Human Ventricular Myocyte Model:

Frontiers in physiology
Avec: Yasutaka Kurata , Kenshi Hayashi , Ichiro Hisatome , Yuhichi Kuda , Mamoru Tanida

Identification of key modules and hub genes for small-cell lung carcinoma and large-cell neuroendocrine lung carcinoma by weighted gene co-expression network analysis of clinical tissue-proteomes.

PloS one
Avec: Haruhiko Nakamura , Kiyonaga Fujii , Vipul Gupta , Hiroko Hata , Hirotaka Koizumu , Masahiro Hoshikawa , Saeko Naruki , Yuka Miyata , Ikuya Takahashi , Tomoyuki Miyazawa , Hiroki Sakai , Kouhei Tsumoto , Masayuki Takagi , Hisashi Saji , Toshihide Nishimura

Highly sensitive biomolecular interaction detection method using optical bound/free separation with grating-coupled surface plasmon field-enhanced fluorescence spectroscopy (GC-SPFS).

PloS one
Avec: Takatoshi Kaya , Satoru Nagatoishi , Kosuke Nagae , Yukito Nakamura , Kohei Tsumoto

Design strategy for serine hydroxymethyltransferase probes based on retro-aldol-type reaction.

Nature communications
Avec: Hiroshi Nonaka , Yuki Nakanishi , Satoshi Kuno , Tomoki Ota , Kentaro Mochidome , Yutaro Saito , Fuminori Sugihara , Yoichi Takakusagi , Ichio Aoki , Satoru Nagatoishi , Kouhei Tsumoto , Shinsuke Sando

Integrative and theoretical research on the architecture of a biological system and its disorder.

The journal of physiological sciences : JPS
Avec: Shinichi Uchida , Yoshiyuki Asai , Yoshiaki Kariya , Hiroshi Hibino , Masashi Honma , Takeshi Abe , Fumiaki Nin , Yasutaka Kurata , Kazuharu Furutani , Hiroshi Suzuki , Hiroaki Kitano , Ryuji Inoue , Yoshihisa Kurachi

Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly.

Frontiers in chemistry
Avec: Hiroki Sakuta , Tadashi Fujimoto , Yusuke Yamana , Yusuke Hoda , Kanta Tsumoto , Kenichi Yoshikawa

Generation and characterization of antagonistic anti-human interleukin (IL)-18 monoclonal antibodies with high affinity: Two types of monoclonal antibodies against full-length IL-18 and the neoepitope of inflammatory caspase-cleaved active IL-18.

Archives of biochemistry and biophysics
Avec: Yuko Nariai , Hiroki Kamino , Eiji Obayashi , Hiroaki Kato , Gyosuke Sakashita , Tomoko Sugiura , Kiyoshi Migita , Tomohiro Koga , Atsushi Kawakami , Kazuma Sakamoto , Kenji Kadomatsu , Makoto Nakakido , Kouhei Tsumoto , Takeshi Urano

Structural and thermodynamical insights into the binding and inhibition of FIH-1 by the N-terminal disordered region of Mint3.

The Journal of biological chemistry
Avec: Tensho Ten , Satoru Nagatoishi , Ryo Maeda , Masaru Hoshino , Yoshiaki Nakayama , Motoharu Seiki , Takeharu Sakamoto , Kouhei Tsumoto

The transcriptional corepressor CtBP2 serves as a metabolite sensor orchestrating hepatic glucose and lipid homeostasis.

Nature communications
Avec: Motohiro Sekiya , Kenta Kainoh , Takehito Sugasawa , Ryunosuke Yoshino , Takatsugu Hirokawa , Hiroaki Tokiwa , Shogo Nakano , Satoru Nagatoishi , Kouhei Tsumoto , Yoshinori Takeuchi , Takafumi Miyamoto , Takashi Matsuzaka , Hitoshi Shimano

Two Secreted Proteoglycans, Activators of Urothelial Cell-Cell Adhesion, Negatively Contribute to Bladder Cancer Initiation and Progression.

Cancers
Avec: Vasiliki Papadaki , Ken Asada , Julie K Watson , Toshiya Tamura , Alex Leung , Jack Hopkins , Margaret Dellett , Noriaki Sasai , Hongorzul Davaapil , Serena Nik-Zainal , Rebecca Longbottom , Makoto Nakakido , Ryo Torii , Abhi Veerakumarasivam , Syuzo Kaneko , Mandeep S Sagoo , Gillian Murphy , Akihisa Mitani , Kohei Tsumoto , John D Kelly , Ryuji Hamamoto , Shin-Ichi Ohnuma

Structural basis for antigen recognition by methylated lysine-specific antibodies.

The Journal of biological chemistry
Avec: Misaki Ishii , Makoto Nakakido , Jose M M Caaveiro , Daisuke Kuroda , C J Okumura , Toshiaki Maruyama , Kevin Entzminger , Kouhei Tsumoto

Characterization of a putative maltodextrin-binding protein of Streptococcus pyogenes, SPs0871 and the development of a VHH inhibitor.

Biochemical and biophysical research communications
Avec: Tsukushi Yamawaki , Makoto Nakakido , Kan Ujiie , Chihiro Aikawa , Ichiro Nakagawa , Kouhei Tsumoto

Biophysical Characterization of the Contribution of the Fab Region to the IgG-FcγRIIIa Interaction.

Biochemistry
Avec: Hirofumi Kosuge , Satoru Nagatoishi , Masato Kiyoshi , Akiko Ishii-Watabe , Yosuke Terao , Teruhiko Ide , Kouhei Tsumoto

Electrofusion of cells with different diameters by generating asymmetrical electric field in the microwell array.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
Avec: Ikumi Onohara , Masato Suzuki , Yushi Isozaki , Kanta Tsumoto , Masahiro Tomita , Tomoyuki Yasukawa

Mechanism of Budded Virus Envelope Fusion into a Planar Bilayer Lipid Membrane on a SiO

Langmuir : the ACS journal of surfaces and colloids
Avec: Azusa Oshima , Kohei Nakanishi , Nahoko Kasai , Hiroshi Nakashima , Kanta Tsumoto , Koji Sumitomo

Single-chain variable fragment (scFv) targeting streptolysin O controls group A Streptococcus infection.

Biochemical and biophysical research communications
Avec: Chihiro Aikawa , Kiyosumi Kawashima , Chihiro Fukuzaki , Makoto Nakakido , Kazunori Murase , Takashi Nozawa , Kouhei Tsumoto , Ichiro Nakagawa

A glutamine sensor that directly activates TORC1.

Communications biology
Avec: Mirai Tanigawa , Katsuyoshi Yamamoto , Satoru Nagatoishi , Koji Nagata , Daisuke Noshiro , Nobuo N Noda , Kouhei Tsumoto , Tatsuya Maeda

Addition of arginine hydrochloride and proline to the culture medium enhances recombinant protein expression in Brevibacillus choshinensis: The case of RBD of SARS-CoV-2 spike protein and its antibody.

Protein expression and purification
Avec: Ryo Matsunaga , Kouhei Tsumoto

Réseau de co-auteurs

Kouhei Tsumoto 62 collaborations
Satoru Nagatoishi 32 collaborations
Makoto Nakakido 22 collaborations
Daisuke Kuroda 11 collaborations
Kanta Tsumoto 10 collaborations
Jose M M Caaveiro 8 collaborations
Hiroki Akiba 6 collaborations
Akinobu Senoo 5 collaborations
Masahiro Tomita 5 collaborations
Hirofumi Kosuge 5 collaborations
Ichiro Nakagawa 5 collaborations
Masato Kiyoshi 4 collaborations
Teruhiko Ide 4 collaborations
Shin-Ichi Ohnuma 4 collaborations
Kohei Tsumoto 4 collaborations
Aki Tanabe 3 collaborations
Yasutaka Kurata 3 collaborations
Takao Hamakubo 3 collaborations
Yasutaka Anraku 3 collaborations
Tsutomu Arakawa 3 collaborations
Takeharu Minamitani 3 collaborations
Teruhito Yasui 3 collaborations
Akiko Ishii-Watabe 3 collaborations
Yosuke Terao 3 collaborations
Yushi Isozaki 3 collaborations
Ken Asada 3 collaborations
Ryuji Hamamoto 3 collaborations
Hiroki Sakuta 3 collaborations
Kenichi Yoshikawa 3 collaborations
Ryo Matsunaga 3 collaborations
Chihiro Aikawa 3 collaborations
Tatsiana Sushko 2 collaborations
Anton Kavaleuski 2 collaborations
Irina Grabovec 2 collaborations
Sergey Bukhdruker 2 collaborations
Egor Marin 2 collaborations
Valentin Borshchevskiy 2 collaborations
Andrei Gilep 2 collaborations
Natallia Strushkevich 2 collaborations
Yoshihisa Kurachi 2 collaborations
Sho Ito 2 collaborations
Horacio Cabral 2 collaborations
Shinsuke Sando 2 collaborations
Kohei Nakanishi 2 collaborations
Osamu Kusano-Arai 2 collaborations
Hiroko Iwanari 2 collaborations
Ryota Otsubo 2 collaborations
Junso Fujita 2 collaborations
Toshihiro Ito 2 collaborations
Keiichi Namba 2 collaborations
Syuzo Kaneko 2 collaborations
Yui Tomioka 2 collaborations
Masataka Nakagawa 2 collaborations
Chiaki Sakuma 2 collaborations
Teruo Akuta 2 collaborations
Tsukushi Yamawaki 2 collaborations
Hiroko Tamura 2 collaborations
Tomoko Ise 2 collaborations
Satoshi Nagata 2 collaborations
Jack Hopkins 2 collaborations
Alex Leung 2 collaborations
Vasiliki Papadaki 2 collaborations
Hongorzul Davaapil 2 collaborations
Akihisa Mitani 2 collaborations
Mandeep S Sagoo 2 collaborations
Kazunori Murase 2 collaborations
Takashi Nozawa 2 collaborations
Keisuke Kasahara 1 collaboration
Raiji Kawade 1 collaboration
Anna Kavaleuskaya 1 collaboration
Daniil Vakhrameev 1 collaboration
Alexey Kuzikov 1 collaboration
Rami Masamrekh 1 collaboration
Victoria Shumyantseva 1 collaboration
Takashi Ashihara 1 collaboration
Narumi Naito 1 collaboration
Takao Shimamoto 1 collaboration
Akira Amano 1 collaboration
Masahito Ohue 1 collaboration
Masaki Yamamoto 1 collaboration
Naoki Wakui 1 collaboration
Yuji Watanabe 1 collaboration
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