Targeted proteomics links virulence factor expression with clinical severity in staphylococcal pneumonia.
Humans
Pneumonia, Staphylococcal
/ microbiology
Staphylococcus aureus
Virulence Factors
/ genetics
Hemoptysis
Proteomics
Exotoxins
/ genetics
Staphylococcal Infections
/ microbiology
Community-Acquired Infections
/ microbiology
Staphylococcus
Leukocidins
/ genetics
Methicillin-Resistant Staphylococcus aureus
/ genetics
Staphylococcus aureus
community-acquired pneumonia (CAP)
multiple reaction monitoring (MRM)
panton valentine leucocidin
tandem mass spectrometer
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2023
2023
Historique:
received:
09
02
2023
accepted:
20
03
2023
medline:
21
4
2023
pubmed:
20
4
2023
entrez:
20
04
2023
Statut:
epublish
Résumé
The bacterial pathogen We present a targeted proteomic approach able to monitor 42 staphylococcal proteins in a single experiment. Using this approach, we compared the quantitative virulomes of 136 We found that leukopenia was predicted by higher expression of HlgB, Nuc, and Tsst-1 and lower expression of BlaI and HlgC, while hemoptysis was predicted by higher expression of BlaZ and HlgB and lower expression of HlgC. Strikingly, mortality was independently predicted in a dose-dependent fashion by a single phage-encoded virulence factor, the Panton-Valentine leucocidin (PVL), both in logistic (OR 1.28; 95%CI[1.02;1.60]) and survival (HR 1.15; 95%CI[1.02;1.30]) regression models. These findings demonstrate that the
Identifiants
pubmed: 37077532
doi: 10.3389/fcimb.2023.1162617
pmc: PMC10106754
doi:
Substances chimiques
Virulence Factors
0
Exotoxins
0
Leukocidins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1162617Informations de copyright
Copyright © 2023 Pivard, Bastien, Macavei, Mouton, Rasigade, Couzon, Youenou, Tristan, Carrière, Moreau, Lemoine and Vandenesch.
Déclaration de conflit d'intérêts
FV and JPR report research funding outside the scope of the present study by bioMérieux. FV reports two patents pending in antimicrobial resistance detection. FV, JL and J-PR report shares in Weezion. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be constructed as a potential conflict of interest.
Références
Med Microbiol Immunol. 2012 Feb;201(1):81-92
pubmed: 21701848
J Med Microbiol. 2017 Oct;66(10):1367-1373
pubmed: 28893360
Eur Respir J. 2021 Nov 11;58(5):
pubmed: 33833037
Nat Rev Microbiol. 2014 Jan;12(1):49-62
pubmed: 24336184
mSphere. 2021 May 19;6(3):
pubmed: 34011681
Mol Microbiol. 2006 Aug;61(4):1038-48
pubmed: 16879652
Nat Microbiol. 2023 Jan;8(1):107-120
pubmed: 36593296
Lancet. 2002 Mar 2;359(9308):753-9
pubmed: 11888586
Am J Respir Crit Care Med. 2014 Nov 15;190(10):1139-48
pubmed: 25303310
Medicina (Kaunas). 2021 Oct 24;57(11):
pubmed: 34833375
MAbs. 2015;7(1):243-54
pubmed: 25523282
Mol Cell Proteomics. 2018 Mar;17(3):442-456
pubmed: 29259044
Nat Commun. 2014 Nov 11;5:5438
pubmed: 25384670
Science. 2013 Nov 15;342(6160):863-6
pubmed: 24233725
Clin Infect Dis. 1999 Nov;29(5):1128-32
pubmed: 10524952
Emerg Infect Dis. 2016 Sep;22(9):1570-8
pubmed: 27224202
Microbiol Spectr. 2019 Jul;7(4):
pubmed: 31267926
Microbiology (Reading). 2021 Dec;167(12):
pubmed: 34928202
iScience. 2022 Feb 07;25(3):103879
pubmed: 35243241
Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5587-92
pubmed: 20231457
Clin Microbiol Infect. 2010 Jul;16(7):979-85
pubmed: 20880412
Mol Syst Biol. 2014 Aug 22;10:747
pubmed: 25149558
J Hosp Infect. 2018 Nov;100(3):309-315
pubmed: 29253623
Annu Rev Microbiol. 2016 Sep 8;70:299-316
pubmed: 27482744
Mol Cell Proteomics. 2014 Mar;13(3):907-17
pubmed: 24443746
Immunol Res. 2014 Aug;59(1-3):182-7
pubmed: 24816557
Clin Microbiol Rev. 2015 Jul;28(3):603-61
pubmed: 26016486
Antimicrob Agents Chemother. 1993 May;37(5):1144-9
pubmed: 8517704
Methods. 2017 Mar 15;117:1-2
pubmed: 28366229
J Leukoc Biol. 2009 Jan;85(1):44-54
pubmed: 18923105
J Bacteriol. 2011 Nov;193(21):6020-31
pubmed: 21908676
J Immunol. 2015 Aug 1;195(3):1034-43
pubmed: 26091719
FEBS J. 2015 May;282(10):2029-44
pubmed: 25754869
Mol Cell Proteomics. 2012 Mar;11(3):M111.013987
pubmed: 22101334
Microbiol Spectr. 2019 Mar;7(2):
pubmed: 30873936
Mol Cell Proteomics. 2021;20:100165
pubmed: 34673283
Nat Rev Microbiol. 2013 Oct;11(10):667-73
pubmed: 24018382
J Bacteriol. 2011 Jun;193(12):2948-58
pubmed: 21478342
Infect Immun. 2011 May;79(5):1927-35
pubmed: 21402769
Cell Host Microbe. 2013 May 15;13(5):584-594
pubmed: 23684309
Nat Microbiol. 2017 Oct;2(10):1381-1388
pubmed: 28785103
Lancet Infect Dis. 2019 Sep;19(9):e313-e321
pubmed: 31151811
Toxins (Basel). 2019 Dec 17;11(12):
pubmed: 31861176