Metasurface-enhanced infrared spectroscopy in multiwell format for real-time assaying of live cells.
Journal
Lab on a chip
ISSN: 1473-0189
Titre abrégé: Lab Chip
Pays: England
ID NLM: 101128948
Informations de publication
Date de publication:
02 05 2023
02 05 2023
Historique:
pmc-release:
02
05
2024
medline:
4
5
2023
pubmed:
4
4
2023
entrez:
3
4
2023
Statut:
epublish
Résumé
Fourier transform infrared (FTIR) spectroscopy is a popular technique for the analysis of biological samples, yet its application in characterizing live cells is limited due to the strong attenuation of mid-IR light in water. Special thin flow cells and attenuated total reflection (ATR) FTIR spectroscopy have been used to mitigate this problem, but these techniques are difficult to integrate into a standard cell culture workflow. In this work, we demonstrate that the use of a plasmonic metasurface fabricated on planar substrates and the probing of cellular IR spectra through metasurface-enhanced infrared spectroscopy (MEIRS) can be an effective technique to characterize the IR spectra of live cells in a high-throughput manner. Cells are cultured on metasurfaces integrated with multiwell cell culture chambers and are probed from the bottom using an inverted FTIR micro-spectrometer. To demonstrate the use of MEIRS as a cellular assay, cellular adhesion on metasurfaces with different surface coatings and cellular response to the activation of the protease-activated receptor (PAR) signaling pathway were characterized through the changes in cellular IR spectra.
Identifiants
pubmed: 37010356
doi: 10.1039/d3lc00017f
pmc: PMC10159923
mid: NIHMS1888701
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2228-2240Subventions
Organisme : NCI NIH HHS
ID : R21 CA251052
Pays : United States
Organisme : NIGMS NIH HHS
ID : R21 GM138947
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002384
Pays : United States
Organisme : NCI NIH HHS
ID : UG3 CA244697
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA194547
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA214274
Pays : United States
Commentaires et corrections
Type : ErratumIn
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