The cryo-EM structure of the CENP-A nucleosome in complex with ggKNL2.
CENP-A
CENP-C-like motif
Cryo-EM
KNL2
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
15 03 2023
15 03 2023
Historique:
revised:
13
01
2023
received:
23
06
2022
accepted:
17
01
2023
pubmed:
7
2
2023
medline:
17
3
2023
entrez:
6
2
2023
Statut:
ppublish
Résumé
Centromere protein A (CENP-A) nucleosomes containing the centromere-specific histone H3 variant CENP-A represent an epigenetic mark that specifies centromere position. The Mis18 complex is a licensing factor for new CENP-A deposition via the CENP-A chaperone, Holliday junction recognition protein (HJURP), on the centromere chromatin. Chicken KINETOCHORE NULL2 (KNL2) (ggKNL2), a Mis18 complex component, has a CENP-C-like motif, and our previous study suggested that ggKNL2 directly binds to the CENP-A nucleosome to recruit HJURP/CENP-A to the centromere. However, the molecular basis for CENP-A nucleosome recognition by ggKNL2 has remained unclear. Here, we present the cryo-EM structure of the chicken CENP-A nucleosome in complex with a ggKNL2 fragment containing the CENP-C-like motif. Chicken KNL2 distinguishes between CENP-A and histone H3 in the nucleosome using the CENP-C-like motif and its downstream region. Both the C-terminal tail and the RG-loop of CENP-A are simultaneously recognized as CENP-A characteristics. The CENP-A nucleosome-ggKNL2 interaction is thus essential for KNL2 functions. Furthermore, our structural, biochemical, and cell biology data indicate that ggKNL2 changes its binding partner at the centromere during chicken cell cycle progression.
Identifiants
pubmed: 36744604
doi: 10.15252/embj.2022111965
pmc: PMC10015371
doi:
Substances chimiques
Autoantigens
0
Cell Cycle Proteins
0
Centromere Protein A
0
Histones
0
Nucleosomes
0
DNA-Binding Proteins
0
Banques de données
PDB
['7Y7I']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e111965Informations de copyright
© 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
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