Analysis of microbial contamination during use and reprocessing of surgical instruments and sterile packaging systems.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2023
2023
Historique:
received:
04
07
2022
accepted:
03
01
2023
entrez:
20
1
2023
pubmed:
21
1
2023
medline:
25
1
2023
Statut:
epublish
Résumé
A surgical site infection (SSI) is one of the most common surgical complications. This study analyzed different sources of microorganisms in the air, on reusable surgical instruments, and the outer surface of sterile packaging systems during the use and reprocessing of sterile goods (from the operating room (OR) to the Central Sterile Supply Department (CSSD)). The microbial load in the air was analyzed via active air sampling and settle plates. Furthermore, the airborne particle load was measured by a particle counter. Contact agar plates were used to determine the microbial load on surgical instruments and sterile packaging systems. The highest average microbial and particle load was measured in the air of the OR (active air sampling: max. 56 CFU/m3; settle plates: max. 9 CFU; ≥0.3 μm particles in size: 1,958,403 no./m3). However, no microbial load (0 CFU) was detected on surgical instruments sampled in the OR. The outer surface of stored sterile packaging systems showed a maximal microbial load of 64 CFU. The most common identified pathogen was coagulase-negative staphylococci. Compared to properly reprocessed reusable surgical instruments and sterile packaging systems, the air still seems to be the primary potential source of microbial contamination, especially within the OR.
Identifiants
pubmed: 36668667
doi: 10.1371/journal.pone.0280595
pii: PONE-D-22-18901
pmc: PMC9858816
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0280595Informations de copyright
Copyright: © 2023 Dreikausen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
Lena Dreikausen, Bernd Blender, and Matthias Henke are employees of Aesculap AG. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Références
J Infect. 2009 Nov;59(5):337-45
pubmed: 19732794
Ann Surg. 1976 Nov;184(5):642-4
pubmed: 984935
Lancet Infect Dis. 2015 Feb;15(2):212-24
pubmed: 25467650
J Hosp Infect. 2000 Dec;46(4):241-56
pubmed: 11170755
Orthop Traumatol Surg Res. 2012 Jun;98(4):432-40
pubmed: 22578871
J Cardiovasc Surg (Torino). 1982 Mar-Apr;23(2):155-62
pubmed: 7085733
J Prev Med Hyg. 2013 Sep;54(3):131-7
pubmed: 24783890
J Hosp Infect. 2005 Sep;61(1):27-9
pubmed: 16009457
Am J Infect Control. 1998 Apr;26(2):143-5
pubmed: 9584809
Clin Orthop Relat Res. 1980 Jul-Aug;(150):177-80
pubmed: 7428217
Am J Infect Control. 2012 Oct;40(8):750-5
pubmed: 22285652
Indian J Surg. 2015 Feb;77(1):16-8
pubmed: 25829705
J Hosp Infect. 1983 Mar;4(1):19-29
pubmed: 6190881
PLoS One. 2012;7(12):e52809
pubmed: 23285189
Ann Surg. 2008 Nov;248(5):695-700
pubmed: 18948793
J Hosp Infect. 2001 Feb;47(2):110-5
pubmed: 11170774
BMC Infect Dis. 2018 Jan 2;18(1):4
pubmed: 29291707
J Hosp Infect. 2000 Feb;44(2):119-26
pubmed: 10662562
J Hosp Infect. 1988 May;11 Suppl C:2-17
pubmed: 2899119
J Bone Joint Surg Am. 2009 Nov;91 Suppl 6:2-9
pubmed: 19884406
Arch Dermatol. 1970 Feb;101(2):145-59
pubmed: 5413252
J Orthop Traumatol. 2011 Dec;12(4):207-11
pubmed: 22072304
Infect Control Hosp Epidemiol. 1999 Apr;20(4):250-78; quiz 279-80
pubmed: 10219875
Infect Control Hosp Epidemiol. 2015 Aug;36(8):990-2
pubmed: 25990555
Am J Infect Control. 2012 Sep;40(7):e228-32
pubmed: 22542026
Clin Orthop Relat Res. 1975 Sep;(111):147-50
pubmed: 1157412
Br J Surg. 1964 Mar;51:195-202
pubmed: 14129433
Infect Control Hosp Epidemiol. 2002 Dec;23(12 Suppl):S3-40
pubmed: 12515399
J Bone Joint Surg Am. 2008 May;90(5):1022-5
pubmed: 18451394
Clin Orthop Relat Res. 1999 Dec;(369):103-9
pubmed: 10611865
PLoS One. 2017 May 22;12(5):e0178007
pubmed: 28531183
Am J Surg. 2009 Aug;198(2):157-62
pubmed: 19285307
Am J Infect Control. 2015 May 1;43(5):522-7
pubmed: 25952049
J Hyg (Lond). 1984 Dec;93(3):505-29
pubmed: 6512253
Clin Microbiol Infect. 2015 Dec;21(12):1047-51
pubmed: 26417851
Infect Control Hosp Epidemiol. 2011 Nov;32(11):1097-102
pubmed: 22011537
AORN J. 2014 May;99(5):600-8; quiz 609-11
pubmed: 24766922
J Hosp Infect. 2004 Nov;58(3):230-2
pubmed: 15501339
J Hosp Infect. 2012 Aug;81(4):231-8
pubmed: 22704634
Am J Infect Control. 2015 Jan;43(1):78-81
pubmed: 25564128
Appl Microbiol. 1962 Nov;10:561-6
pubmed: 13950172
J Hosp Infect. 2001 Feb;47(2):104-9
pubmed: 11170773
J Hosp Infect. 2017 Dec;97(4):348-352
pubmed: 28647423
PLoS Med. 2016 Oct 18;13(10):e1002150
pubmed: 27755545
Am J Infect Control. 2014 Jan;42(1):43-7
pubmed: 24189327
Med Sci Monit. 2015 Aug 13;21:2367-74
pubmed: 26269120