Sportomics method to assess acute phase proteins in Olympic level athletes using dried blood spots and multiplex assay.

Humans Reproducibility of Results Acute-Phase Proteins Sports C-Reactive Protein / metabolism Athletes Mannose-Binding Lectin

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
18 11 2022
Historique:
received: 22 04 2022
accepted: 29 10 2022
pubmed: 19 11 2022
medline: 23 11 2022
entrez: 18 11 2022
Statut: epublish

Résumé

Sportomics is a subject-centered holistic method similar to metabolomics focusing on sports as the metabolic challenge. Dried blood spot is emerging as a technique due to its simplicity and reproducibility. In addition, mass spectrometry and integrative computational biology enhance our ability to understand exercise-induced modifications. We studied inflammatory blood proteins (Alpha-1-acid glycoprotein-A1AG1; Albumin; Cystatin C; C-reactive protein-CRP; Hemoglobin-HBA; Haptoglobin-HPT; Insulin-like growth factor 1; Lipopolysaccharide binding protein-LBP; Mannose-binding lectin-MBL2; Myeloperoxidase-PERM and Serum amyloid A1-SAA1), in 687 samples from 97 World-class and Olympic athletes across 16 sports in nine states. Data were analyzed with Spearman's rank-order correlation. Major correlations with CRP, LBP; MBL2; A1AG1, and SAA1 were found. The pairs CRP-SAA1 and CRP-LBP appeared with a robust positive correlation. Other pairs, LBP-SAA1; A1AG1-CRP; A1AG1-SAA1; A1AG1-MBL, and A1AG1-LBP, showed a broader correlation across the sports. The protein-protein interaction map revealed 1500 interactions with 44 core proteins, 30 of them linked to immune system processing. We propose that the inflammation follow-up in exercise can provide knowledge for internal cargo management in training, competition, recovery, doping control, and a deeper understanding of health and disease.

Identifiants

DOI: 10.1038/s41598-022-23300-y PMID: 36400821 PMC: PMC9672598
pubmed: 36400821
doi: 10.1038/s41598-022-23300-y
pii: 10.1038/s41598-022-23300-y
pmc: PMC9672598
doi:

Substances chimiques

Acute-Phase Proteins 0
C-Reactive Protein 9007-41-4
MBL2 protein, human 0
Mannose-Binding Lectin 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

19824

Informations de copyright

© 2022. The Author(s).

Références

Arch Biochem Biophys. 2018 Feb 15;640:47-52
pubmed: 29336940
Cells. 2020 Aug 17;9(8):
pubmed: 32824536
J Gerontol A Biol Sci Med Sci. 2012 Nov;67(11):1212-8
pubmed: 22960476
Int J Sports Physiol Perform. 2020 Sep 22;:
pubmed: 32963119
EMBO Rep. 2018 Oct;19(10):
pubmed: 30126923
J Lab Clin Med. 1998 Nov;132(5):414-20
pubmed: 9823935
Blood Cells Mol Dis. 2009 May-Jun;42(3):294-9
pubmed: 19233694
J Physiol Biochem. 2015 Jun;71(2):329-40
pubmed: 25711902
Proteomes. 2018 Sep 01;6(3):
pubmed: 30200480
Biochem Biophys Res Commun. 2014 Mar 21;445(4):708-16
pubmed: 24406165
Nutrients. 2016 Nov 11;8(11):
pubmed: 27845704
PLoS One. 2015 Dec 04;10(12):e0144279
pubmed: 26636573
Inflammation. 1997 Feb;21(1):69-82
pubmed: 9179623
OMICS. 2011 Oct;15(10):695-704
pubmed: 21978397
Int J Sports Physiol Perform. 2020 Apr 25;:1-12
pubmed: 32335533
Front Pharmacol. 2016 Sep 13;7:302
pubmed: 27679573
Int J Sports Med. 2015 Mar;36(3):262-70
pubmed: 25525952
J Sports Sci. 2019 May;37(9):959-967
pubmed: 28346122
J Biol Chem. 2010 Jul 16;285(29):22174-85
pubmed: 20442402
Cytokine Growth Factor Rev. 2016 Aug;30:55-69
pubmed: 26794452
Nucleic Acids Res. 2022 Jan 7;50(D1):D640-D647
pubmed: 34755877
Aliment Pharmacol Ther. 2017 Aug;46(3):246-265
pubmed: 28589631
Immunol Lett. 2015 May;165(1):10-9
pubmed: 25794633
Pharm Res. 2018 Dec 28;36(2):30
pubmed: 30593605
J Int Soc Sports Nutr. 2012 Jun 26;9(1):30
pubmed: 22734448
PLoS Pathog. 2019 Mar 7;15(3):e1007381
pubmed: 30845179
J Mol Cell Biol. 2021 Mar 10;12(12):916-932
pubmed: 33295606
Clin Chem Lab Med. 2010 Feb;48(2):199-203
pubmed: 20001441
Clin Sci (Lond). 1991 Nov;81(5):677-83
pubmed: 1721863
Front Immunol. 2018 Apr 13;9:754
pubmed: 29706967
Vox Sang. 2015 Feb;108(2):131-40
pubmed: 25200932
Annu Rev Food Sci Technol. 2019 Mar 25;10:341-363
pubmed: 30633566
Sports Med. 2021 Jan;51(1):113-124
pubmed: 33201454
Proteomics Clin Appl. 2010 May;4(5):568-76
pubmed: 21137074
Cell. 2020 Dec 23;183(7):2036-2039
pubmed: 33357400
J Infect. 2020 Jun;80(6):646-655
pubmed: 32277967
Eur Rev Med Pharmacol Sci. 2020 Nov;24(21):11386-11394
pubmed: 33215460
Bioanalysis. 2016 Aug;8(15):1597-1609
pubmed: 27420772
Bioinformatics. 2009 Dec 15;25(24):3327-9
pubmed: 19837718
Int J Clin Pract. 2019 Oct;73(10):e13385
pubmed: 31243854
PLoS One. 2012;7(8):e41387
pubmed: 22916107
Infect Immun. 2001 Nov;69(11):6942-50
pubmed: 11598069
Clin J Sport Med. 2001 Jan;11(1):38-43
pubmed: 11176144
J Sports Med Phys Fitness. 2021 Feb;61(2):324-330
pubmed: 32936572
Mol Nutr Food Res. 2019 Oct;63(19):e1900167
pubmed: 31298466
J Biol Chem. 2003 Aug 1;278(31):28367-71
pubmed: 12754215
Br J Sports Med. 2008 Nov;42(11):889-93
pubmed: 18203867
Eur Rev Med Pharmacol Sci. 2019 Dec;23(24):11011-11019
pubmed: 31858572
Curr Med Chem. 2016;23(17):1725-55
pubmed: 27087246
Eur J Vasc Endovasc Surg. 1997 Nov;14(5):344-50
pubmed: 9413374
Diabetes. 2016 Jun;65(6):1630-41
pubmed: 27207522
J Sport Health Sci. 2020 Sep;9(5):432-445
pubmed: 32928447
Am J Transl Res. 2020 Aug 15;12(8):4569-4575
pubmed: 32913530
Clin Chem. 1993 Feb;39(2):293-7
pubmed: 8381732
Am J Respir Cell Mol Biol. 1992 Sep;7(3):239-45
pubmed: 1381594
Cytokine. 2020 Dec;136:155273
pubmed: 32932194
Proteomics. 2012 May;12(10):1478-98
pubmed: 22711592
Physiol Rev. 2000 Jul;80(3):1055-81
pubmed: 10893431
Nat Immunol. 2010 Nov;11(11):1039-46
pubmed: 20890286
Sci Rep. 2016 Jan 07;6:18839
pubmed: 26740279
Bioanalysis. 2020 Jul;12(13):937-955
pubmed: 32253915
Front Physiol. 2020 May 25;11:373
pubmed: 32523539
Temperature (Austin). 2019 May 7;7(1):58-88
pubmed: 32166105
Autoimmun Rev. 2007 Jan;6(3):169-75
pubmed: 17289553

Auteurs

Adriana Bassini (A)

Laboratory of Protein Biochemistry, Federal University of State of Rio de Janeiro, Av. Pasteur, 296 - Urca, Rio de Janeiro, R.J., 22290-350, Brazil.
SOmics, Vila Velha, ES, Brazil.

Silvia Sartoretto (S)

Laboratory of Protein Biochemistry, Federal University of State of Rio de Janeiro, Av. Pasteur, 296 - Urca, Rio de Janeiro, R.J., 22290-350, Brazil.

Lukas Jurisica (L)

Laboratory of Protein Biochemistry, Federal University of State of Rio de Janeiro, Av. Pasteur, 296 - Urca, Rio de Janeiro, R.J., 22290-350, Brazil.
School of Computer Science, Carleton University, Ottawa, Canada.
Department of Computer Science, University of Toronto, Toronto, Canada.

Alexandre Magno-França (A)

Laboratory of Protein Biochemistry, Federal University of State of Rio de Janeiro, Av. Pasteur, 296 - Urca, Rio de Janeiro, R.J., 22290-350, Brazil.

Leigh Anderson (L)

SISCAPA Assay Technologies, Inc., Washington, DC, USA.

Terry Pearson (T)

SISCAPA Assay Technologies, Inc., Washington, DC, USA.

Morty Razavi (M)

SISCAPA Assay Technologies, Inc., Washington, DC, USA.

Vinod Chandran (V)

Arthritis Program, Schroeder Arthritis Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada.
Faculty of Medicine, University of Toronto, Toronto, ON, Canada.

LeRoy Martin (L)

Waters Technologies, Milford, MA, USA.

Igor Jurisica (I)

Osteoarthritis Research Program, Division of Orthopedic Surgery, Schroeder Arthritis Institute, and Data Science Discovery Centre for Chronic Diseases, Krembil Research Institute, University Health Network, Toronto, Canada. juris@ai.utoronto.ca.
Departments of Medical Biophysics and Computer Science, and Faculty of Dentistry, University of Toronto, Toronto, ON, Canada. juris@ai.utoronto.ca.
Institute of Neuroimmunology, Slovak Academy of Sciences, Bratislava, Slovakia. juris@ai.utoronto.ca.

L C Cameron (LC)

Laboratory of Protein Biochemistry, Federal University of State of Rio de Janeiro, Av. Pasteur, 296 - Urca, Rio de Janeiro, R.J., 22290-350, Brazil. cameron@unirio.br.

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