Evaluating the effect of LPS from periodontal pathogenic bacteria on the expression of senescence-related genes in human dental pulp stem cells.
CDKN1A
CDKN2A
DPSCs
LPS
SIRT1
TP53
senescence
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
revised:
26
09
2022
received:
05
12
2021
accepted:
30
09
2022
pubmed:
20
10
2022
medline:
19
11
2022
entrez:
19
10
2022
Statut:
ppublish
Résumé
The human dental pulp stem cells (hDPSCs) are one of the readily available sources of multipotent mesenchymal stem cells (MSCs) and can be considered as a type of tool cells for cell-based therapies. However, the main limitation in the clinical use of these cells is DPSC senescence, which can be induced by lipopolysaccharide (LPS) of oral pathogenic bacteria. Up to now, far little attention has been paid to exploring the molecular mechanisms of senescence in DPSCs. So, the current study aimed to investigate the underlying molecular mechanism of senescence in hDPSCs stimulated with Porphyromonas gingivalis (P. gingivalis) and Escherichia coli (E. coli)-derived LPSs, by evaluating both mRNA and protein expression of four important senescence-related genes, including TP53, CDKN1A, CDKN2A and SIRT1. To this purpose, hDPSCs were stimulated with different LPSs for 6, 24 and 48 h and then the gene expression was evaluated using quantitative real-time polymerase chain reaction (qPCR) and western blotting. Following stimulation with P. gingivalis and E. coli-derived LPSs, the relative mRNA and protein expression of all genes were significantly up-regulated in a time-dependent manner, as compared with unstimulated hDPSCs. Moreover, the hDPSCs stimulated with P. gingivalis LPS for 6 and 24 h had the highest mRNA expression of CDKN1A and SIRT1, respectively (p < 0.0001), whereas the highest mRNA expression of CDKN2A and TP53 was seen in hDPSCs stimulated with E. coli LPS for 48 h (p < 0.0001). In summary, because DPSCs have been reported to have therapeutic potential for several cell-based therapies, targeting molecular mechanisms aiming at preventing DPSC senescence could be considered a valuable strategy.
Identifiants
pubmed: 36259309
doi: 10.1111/jcmm.17594
pmc: PMC9667521
doi:
Substances chimiques
Lipopolysaccharides
0
Sirtuin 1
EC 3.5.1.-
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5647-5656Subventions
Organisme : Shahid Beheshti University of Medical Sciences
ID : 971372
Informations de copyright
© 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
Références
Int J Cancer. 2012 Apr 15;130(8):1715-25
pubmed: 22025288
Stem Cell Res Ther. 2016 Mar 22;7:44
pubmed: 27006071
Neurobiol Aging. 2019 Aug;80:127-137
pubmed: 31170533
J Cell Biochem. 2018 Oct 15;:
pubmed: 30324689
Cell Stress Chaperones. 2020 May;25(3):503-508
pubmed: 32253738
Ageing Res Rev. 2018 May;43:64-80
pubmed: 29476819
Cytotechnology. 2018 Jun;70(3):1023-1035
pubmed: 29480340
Adv Protein Chem Struct Biol. 2020;120:45-84
pubmed: 32085888
J Endod. 2017 Sep;43(9S):S12-S16
pubmed: 28781091
J Dent Res. 2016 Nov;95(12):1333-1340
pubmed: 27302881
Acta Pharmacol Sin. 2016 Aug;37(8):1031-44
pubmed: 27345627
J Cell Mol Med. 2022 Nov;26(22):5647-5656
pubmed: 36259309
Int J Mol Sci. 2019 Nov 29;20(23):
pubmed: 31795382
Exp Gerontol. 2018 May;105:10-18
pubmed: 29275161
Curr Opin Rheumatol. 2019 Sep;31(5):517-524
pubmed: 31268867
Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4291-6
pubmed: 10760295
Life Sci. 2019 Jan 1;216:111-118
pubmed: 30465790
Neural Regen Res. 2014 Jan 1;9(1):10-5
pubmed: 25206738
Cell Res. 2002 Sep;12(3-4):229-33
pubmed: 12296382
Oncogene. 2013 Oct 24;32(43):5129-43
pubmed: 23416979
Transplantation. 2005 Sep 27;80(6):836-42
pubmed: 16210973
Endocr Relat Cancer. 2013 Aug 23;20(5):R269-90
pubmed: 23975882
Oncogene. 2017 Jan 12;36(2):219-230
pubmed: 27238838
Am J Respir Crit Care Med. 2012 Aug 15;186(4):306-13
pubmed: 22582162
Genes Dev. 2014 Jan 15;28(2):99-114
pubmed: 24449267
Cell Death Differ. 2003 Apr;10(4):431-42
pubmed: 12719720
Development. 2016 Jul 1;143(13):2273-80
pubmed: 27381225
Science. 1997 Aug 8;277(5327):831-4
pubmed: 9242615
J Clin Pathol. 2018 Oct;71(10):853-858
pubmed: 30076191
Aging Cell. 2003 Dec;2(6):295-304
pubmed: 14677632
Mech Ageing Dev. 2020 Apr;187:111215
pubmed: 32084459
J Immunol Res. 2014;2014:476068
pubmed: 24741603
J Cell Biochem. 2019 Sep;120(9):15746-15755
pubmed: 31069840
Oncogene. 1999 May 6;18(18):2789-97
pubmed: 10362249
Oxid Med Cell Longev. 2019 May 22;2019:6492029
pubmed: 31223423
Curr Biol. 1998 Mar 12;8(6):351-4
pubmed: 9512419
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014 Sep;158(3):373-7
pubmed: 24145770
Am J Obstet Gynecol. 2010 May;202(5):471.e1-11
pubmed: 20452492
J Leukoc Biol. 2007 Jan;81(1):1-5
pubmed: 17032697
BMC Dev Biol. 2015 Mar 14;15:14
pubmed: 25879198
Cell Tissue Res. 2014 May;356(2):369-80
pubmed: 24676500
J Atheroscler Thromb. 2020 Jan 1;27(1):47-59
pubmed: 31105126