XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 08 2022
13 08 2022
Historique:
received:
23
04
2021
accepted:
29
06
2022
entrez:
13
8
2022
pubmed:
14
8
2022
medline:
17
8
2022
Statut:
epublish
Résumé
Cells employ global genome nucleotide excision repair (GGR) to eliminate a broad spectrum of DNA lesions, including those induced by UV light. The lesion-recognition factor XPC initiates repair of helix-destabilizing DNA lesions, but binds poorly to lesions such as CPDs that do not destabilize DNA. How difficult-to-repair lesions are detected in chromatin is unknown. Here, we identify the poly-(ADP-ribose) polymerases PARP1 and PARP2 as constitutive interactors of XPC. Their interaction results in the XPC-stimulated synthesis of poly-(ADP-ribose) (PAR) by PARP1 at UV lesions, which in turn enables the recruitment and activation of the PAR-regulated chromatin remodeler ALC1. PARP2, on the other hand, modulates the retention of ALC1 at DNA damage sites. Notably, ALC1 mediates chromatin expansion at UV-induced DNA lesions, leading to the timely clearing of CPD lesions. Thus, we reveal how chromatin containing difficult-to-repair DNA lesions is primed for repair, providing insight into mechanisms of chromatin plasticity during GGR.
Identifiants
pubmed: 35963869
doi: 10.1038/s41467-022-31820-4
pii: 10.1038/s41467-022-31820-4
pmc: PMC9376112
doi:
Substances chimiques
Chromatin
0
DNA-Binding Proteins
0
Poly(ADP-ribose) Polymerase Inhibitors
0
Poly Adenosine Diphosphate Ribose
26656-46-2
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
4762Subventions
Organisme : NCI NIH HHS
ID : P01 CA092584
Pays : United States
Organisme : NIH HHS
ID : P40 OD010440
Pays : United States
Informations de copyright
© 2022. The Author(s).
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