High-efficient serum-free differentiation of endothelial cells from human iPS cells.

Animals Cell Differentiation / physiology Endothelial Cells / metabolism Humans Induced Pluripotent Stem Cells / metabolism Mesoderm / metabolism Vascular Endothelial Growth Factor A / metabolism
3D scalable bioreactor suspension culture Angiogenesis Arterial endothelium Differentiation Endothelial cells Human induced pluripotent stem cells LDL uptake Regenerative medicine, 2D monolayer culture Vascular endothelium Wnt signaling eNOS hiPSCs iPS cells

Journal

Stem cell research & therapy
ISSN: 1757-6512
Titre abrégé: Stem Cell Res Ther
Pays: England
ID NLM: 101527581

Informations de publication

Date de publication:
11 06 2022
Historique:
received: 17 01 2022
accepted: 29 05 2022
entrez: 11 6 2022
pubmed: 12 6 2022
medline: 15 6 2022
Statut: epublish

Résumé

Endothelial cells (ECs) form the inner lining of all blood vessels of the body play important roles in vascular tone regulation, hormone secretion, anticoagulation, regulation of blood cell adhesion and immune cell extravasation. Limitless ECs sources are required to further in vitro investigations of ECs' physiology and pathophysiology as well as for tissue engineering approaches. Ideally, the differentiation protocol avoids animal-derived components such as fetal serum and yields ECs at efficiencies that make further sorting obsolete for most applications. Human induced pluripotent stem cells (hiPSCs) are cultured under serum-free conditions and induced into mesodermal progenitor cells via stimulation of Wnt signaling for 24 h. Mesodermal progenitor cells are further differentiated into ECs by utilizing a combination of human vascular endothelial growth factor A165 (VEGF), basic fibroblast growth factor (bFGF), 8-Bromoadenosine 3',5'-cyclic monophosphate sodium salt monohydrate (8Bro) and melatonin (Mel) for 48 h. This combination generates hiPSC derived ECs (hiPSC-ECs) at a fraction of 90.9 ± 1.5% and is easily transferable from the two-dimensional (2D) monolayer into three-dimensional (3D) scalable bioreactor suspension cultures. hiPSC-ECs are positive for CD31, VE-Cadherin, von Willebrand factor and CD34. Furthermore, the majority of hiPSC-ECs express the vascular endothelial marker CD184 (CXCR4). The differentiation method presented here generates hiPSC-ECs in only 6 days, without addition of animal sera and at high efficiency, hence providing a scalable source of hiPSC-ECs.

Identifiants

DOI: 10.1186/s13287-022-02924-x PMID: 35690874 PMC: PMC9188069
pubmed: 35690874
doi: 10.1186/s13287-022-02924-x
pii: 10.1186/s13287-022-02924-x
pmc: PMC9188069
doi:

Substances chimiques

Vascular Endothelial Growth Factor A 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

251

Informations de copyright

© 2022. The Author(s).

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Auteurs

Sarkawt Hamad (S)

Medical Faculty, Center for Physiology and Pathophysiology, Institute for Neurophysiology, University of Cologne, Robert Koch Str. 39, 50931, Cologne, Germany.
Biology Department, Faculty of Science, Soran University, Kurdistan Region, Soran, Iraq.

Daniel Derichsweiler (D)

Medical Faculty, Center for Physiology and Pathophysiology, Institute for Neurophysiology, University of Cologne, Robert Koch Str. 39, 50931, Cologne, Germany.

John Antonydas Gaspar (JA)

Medical Faculty, Center for Physiology and Pathophysiology, Institute for Neurophysiology, University of Cologne, Robert Koch Str. 39, 50931, Cologne, Germany.

Konrad Brockmeier (K)

Department of Pediatric Cardiology, University Hospital of Cologne, Cologne, Germany.

Jürgen Hescheler (J)

Medical Faculty, Center for Physiology and Pathophysiology, Institute for Neurophysiology, University of Cologne, Robert Koch Str. 39, 50931, Cologne, Germany.

Agapios Sachinidis (A)

Medical Faculty, Center for Physiology and Pathophysiology, Institute for Neurophysiology, University of Cologne, Robert Koch Str. 39, 50931, Cologne, Germany.
Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany.

Kurt Paul Pfannkuche (KP)

Medical Faculty, Center for Physiology and Pathophysiology, Institute for Neurophysiology, University of Cologne, Robert Koch Str. 39, 50931, Cologne, Germany. Kurt.Pfannkuche@uni-koeln.de.
Department of Pediatric Cardiology, University Hospital of Cologne, Cologne, Germany. Kurt.Pfannkuche@uni-koeln.de.
Marga-and-Walter-Boll Laboratory for Cardiac Tissue Engineering, University of Cologne, Cologne, Germany. Kurt.Pfannkuche@uni-koeln.de.
Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany. Kurt.Pfannkuche@uni-koeln.de.
ORCID: 0000-0002-4284-774X

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Classifications MeSH

Recherchemedicale.com › Eucaryotes › Animaux › High-efficient serum-free differentiation of...
Recherchemedicale.com › Phénomènes physiologiques cellulaires › Différenciation cellulaire › High-efficient serum-free differentiation of...
Recherchemedicale.com › Cellules › Cellules épithéliales › Cellules endothéliales › High-efficient serum-free differentiation of...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › High-efficient serum-free differentiation of...
Recherchemedicale.com › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › High-efficient serum-free differentiation of...
Recherchemedicale.com › Structures de l'embryon › Feuillets embryonnaires › Mésoderme › High-efficient serum-free differentiation of...
Recherchemedicale.com › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Protéines angiogéniques › Facteurs de croissance endothéliale vasculaire › Facteur de croissance endothéliale vasculaire de type A › High-efficient serum-free differentiation of...