Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds.
Benzoic acids
Jasmonic acid
LC-MS
Salicylic acid
Secondary metabolism
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
3
6
2022
pubmed:
4
6
2022
medline:
9
6
2022
Statut:
ppublish
Résumé
Measuring quantitative changes in plant hormones and derivatives is crucial to understand how reactive oxygen species trigger signaling cascades to regulate stress responses. In this chapter, we describe the liquid chromatography-mass spectrometry procedure that we use to extract and quantify salicylic acid (SA), jasmonic acid (JA), and related compounds in common extracts of Arabidopsis tissue. The method can provide quantitative data on SA, SA glucosides, and JA, as well as information on oxidized and conjugated forms of these compounds and related derivatives of benzoic acid.
Identifiants
pubmed: 35657523
doi: 10.1007/978-1-0716-2469-2_16
doi:
Substances chimiques
Cyclopentanes
0
Oxylipins
0
Plant Growth Regulators
0
Salicylic Acid
O414PZ4LPZ
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
215-223Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
Références
Mhamdi A, Van Breusegem F (2018) Reactive oxygen species in plant development. Development 145
Noctor G, Reichheld JP, Foyer CH (2018) ROS-related signaling in plants. Semin Cell Dev Biol 80:3–12
doi: 10.1016/j.semcdb.2017.07.013
Vanacker H, Carver TLW, Foyer CH (2000) Early H
doi: 10.1104/pp.123.4.1289
Laloi C, Mestres-Ortega MY, Meyer Y, Reichheld JP (2004) The Arabidopsis cytosolic thioredoxin h5 gene induction by oxidative stress and its W-box-mediated response to pathogen elicitor. Plant Physiol 134:1006–1016
doi: 10.1104/pp.103.035782
Mou Z, Fan WH, Dong XN (2003) Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes. Cell 113:935–944
doi: 10.1016/S0092-8674(03)00429-X
Mhamdi A, Hager J, Chaouch S et al (2010) Arabidopsis GLUTATHIONE REDUCTASE 1 is essential for the metabolism of intracellular H
doi: 10.1104/pp.110.153767
Han Y, Chaouch S, Mhamdi A et al (2013) Functional analysis of Arabidopsis mutants points to novel roles for glutathione in coupling H
doi: 10.1089/ars.2012.5052
Han Y, Mhamdi A, Chaouch S, Noctor G (2013) Regulation of basal and oxidative stress-triggered jasmonic acid-related gene expression by glutathione. Plant Cell Environ 36:1135–1146
doi: 10.1111/pce.12048
Dempsey DMA, Vlot AC, Wildermuth MC, Klessig DF (2011) Salicylic acid biosynthesis and metabolism. The Arabidopsis Book 9:e0156
doi: 10.1199/tab.0156
Miersch O, Neumerkel J, Dippe M, Stenzel I, Wasternack C (2008) Hydroxylated jasmonates are commonly occurring metabolites of jasmonic acid and contribute to a partial switch-off in jasmonate signalling. New Phytol 177:114–127
doi: 10.1111/j.1469-8137.2007.02252.x
Acosta IF, Farmer EE (2010) Jasmonates. The Arabidopsis Book 8:e0129
doi: 10.1199/tab.0129
Bartsch M, Bednarek P, Vivancos PD et al (2010) Accumulation of isochorismate-derived 2,3-dihydroxybenzoic 3-O-β-d-xyloside in Arabidopsis resistance to pathogens and ageing of leaves. J Biol Chem 285:25654–25665
doi: 10.1074/jbc.M109.092569
Le Roux C, Del Prete S, Boutet-Mercey S, Perreau F, Balagué C, Roby D, Fagard M, Gaudin V (2014) The hnRNP-Q protein LIF2 participates in the plant immune response. PLoS One 9:e99343
doi: 10.1371/journal.pone.0099343