Esrrb is a cell-cycle-dependent associated factor balancing pluripotency and XEN differentiation.
ChIP-seq and single-cell RNA-seq (scRNA-seq)
Esrrb transcription factor
Exit from pluripotency
cell cycle
cellular differentiation and lineage specification
embryonic stem cells
epiblast stem cells (EpiSC)
extraembryonic endoderm stem cells(XEN)
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
14 06 2022
14 06 2022
Historique:
received:
08
02
2022
revised:
25
04
2022
accepted:
25
04
2022
pubmed:
21
5
2022
medline:
18
6
2022
entrez:
20
5
2022
Statut:
ppublish
Résumé
Cell cycle and differentiation decisions are linked; however, the underlying principles that drive these decisions are unclear. Here, we combined cell-cycle reporter system and single-cell RNA sequencing (scRNA-seq) profiling to study the transcriptomes of embryonic stem cells (ESCs) in the context of cell-cycle states and differentiation. By applying retinoic acid, to G1 and G2/M ESCs, we show that, while both populations can differentiate toward epiblast stem cells (EpiSCs), only G2/M ESCs could differentiate into extraembryonic endoderm cells. We identified Esrrb, a pluripotency factor that is upregulated during G2/M, as a driver of extraembryonic endoderm stem cell (XEN) differentiation. Furthermore, enhancer chromatin states based on wild-type (WT) and ESRRB knockout (KO) ESCs show association of ESRRB with XEN poised enhancers. G1 cells overexpressing Esrrb allow ESCs to produce XENs, while ESRRB-KO ESCs lost their potential to differentiate into XEN. Overall, this study reveals a vital link between Esrrb and cell-cycle states during the exit from pluripotency.
Identifiants
pubmed: 35594859
pii: S2213-6711(22)00205-3
doi: 10.1016/j.stemcr.2022.04.016
pmc: PMC9214067
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1334-1350Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
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