A bovine miRNA, bta-miR-154c, withstands in vitro human digestion but does not affect cell viability of colorectal human cell lines after transfection.
XenomiRs
bovine
colorectal cancer
digestion
epithelial cell lines
miroRNAs
Journal
FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
revised:
02
02
2022
received:
14
01
2021
accepted:
21
03
2022
pubmed:
24
3
2022
medline:
6
5
2022
entrez:
23
3
2022
Statut:
ppublish
Résumé
Colorectal cancer (CRC) is the third most frequent human cancer with over 1.3 million new cases globally. CRC is a complex disease caused by interactions between genetic and environmental factors; in particular, high consumption of red meat, including beef, is considered a risk factor for CRC initiation and progression. Recent data demonstrate that exogenous microRNAs (miRNAs) entering the body via ingestion could pose an effect on the consumer. In this study, we focused on bovine miRNAs that do not share a seed sequence with humans and mice. We identified bta-miR-154c, a bovine miRNA found in edible parts of beef and predicted via cross-species bioinformatic analysis to affect cancer-related pathways in human cells. When bovine tissue was subjected to cooking and a simulation of human digestion, bta-miR-154c was still detected after all procedures, albeit at reduced concentrations. However, lipofection of bta-miR-154c in three different colorectal human cell lines did not affect their viability as evaluated at various time points and concentrations. These data indicate that bta-miR-154c (a) may affect cancer-related pathways in human cells, (b) can withstand digestion and be detected after all stages of an in vitro digestion protocol, but (c) it does not appear to alter epithelial cell viability after entering human enterocytes, even at supraphysiological amounts. Further experiments will elucidate whether bta-miR-154c exerts a different functional effect on the human gut epithelium, which may cause it to contribute to CRC progression through its consumption.
Identifiants
pubmed: 35318810
doi: 10.1002/2211-5463.13402
pmc: PMC9063428
doi:
Substances chimiques
MicroRNAs
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
925-936Subventions
Organisme : Universitas Foundation
ID : UFF/2016/24
Organisme : University of Nicosia Seed
ID : SeedGrant/2020/50
Informations de copyright
© 2022 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
Références
Int J Mol Sci. 2021 Jan 22;22(3):
pubmed: 33499350
J Nutr. 2015 Oct;145(10):2201-6
pubmed: 26269243
Nutr Res. 2018 Dec;60:68-76
pubmed: 30527261
Nutrients. 2018 Feb 15;10(2):
pubmed: 29462875
J Agric Food Chem. 2021 Aug 18;69(32):9326-9337
pubmed: 34358423
mSystems. 2018 May 15;3(3):
pubmed: 29795787
Curr Mol Pharmacol. 2018;11(2):90-96
pubmed: 29034844
RNA Biol. 2013 Jul;10(7):1107-16
pubmed: 23669076
Nat Biotechnol. 2013 Nov;31(11):965-7
pubmed: 24213763
Int J Cancer. 2012 Jun 1;130(11):2475-83
pubmed: 22212999
J Nutr Biochem. 2020 Jul;81:108376
pubmed: 32330841
J Nutr. 2016 Nov;146(11):2206-2215
pubmed: 27708120
Cancer Prev Res (Phila). 2014 Aug;7(8):786-95
pubmed: 25092886
Semin Cancer Biol. 2021 Aug;73:19-29
pubmed: 33086083
Mol Nutr Food Res. 2017 Nov;61(11):
pubmed: 28688106
RNA Biol. 2013 Jul;10(7):1080-6
pubmed: 23770773
J Nutr. 2014 Oct;144(10):1495-500
pubmed: 25122645
PLoS One. 2014 Feb 28;9(2):e90244
pubmed: 24587298
Exp Biol Med (Maywood). 2017 Apr;242(8):813-839
pubmed: 28205448
Noncoding RNA Res. 2020 Aug 23;5(3):125-134
pubmed: 32954092
BMC Bioinformatics. 2015 May 22;16:169
pubmed: 25994840
Cell Res. 2012 Jan;22(1):107-26
pubmed: 21931358
Genes Nutr. 2017 Jun 22;12:15
pubmed: 28694875
Nutr Metab (Lond). 2018 Oct 1;15:68
pubmed: 30302122
J Biol Chem. 2015 Sep 25;290(39):23680-91
pubmed: 26240150
Mol Nutr Food Res. 2019 Jan;63(2):e1800076
pubmed: 30378765
Cell Res. 2016 Feb;26(2):217-28
pubmed: 26794868
Cell Host Microbe. 2018 Nov 14;24(5):637-652.e8
pubmed: 30449315
Sci Rep. 2018 Jul 6;8(1):10253
pubmed: 29980707
PeerJ. 2020 Sep 17;8:e9567
pubmed: 32995073
Gut. 2011 Mar;60(3):397-411
pubmed: 21036793
Oncotarget. 2020 Apr 07;11(14):1203-1217
pubmed: 32292571
J Agric Food Chem. 2017 Nov 1;65(43):9506-9513
pubmed: 28967249
J Nutr Biochem. 2015 May;26(5):505-12
pubmed: 25704478
Cell Host Microbe. 2016 Jan 13;19(1):32-43
pubmed: 26764595
Mutat Res. 1997 May 12;376(1-2):253-9
pubmed: 9202762
PLoS One. 2015 Sep 22;10(9):e0138275
pubmed: 26394052
J Gastrointest Oncol. 2011 Dec;2(4):215-22
pubmed: 22811855
Biochim Biophys Acta Rev Cancer. 2020 Jan;1873(1):188334
pubmed: 31783067
RNA Biol. 2016 Nov;13(11):1103-1116
pubmed: 27645402
Bioessays. 2014 Apr;36(4):394-406
pubmed: 24436255
RNA Biol. 2012 Oct;9(10):1275-87
pubmed: 22995834
Food Funct. 2014 Jun;5(6):1113-24
pubmed: 24803111