Photodynamic Effects with 5-Aminolevulinic Acid on Cytokines and Exosomes in Human Peripheral Blood Mononuclear Cells.
5-aminolevulinic acid (ALA)
cytokine
electron microscopy
exosome
flow cytometry
peripheral blood mononuclear cells (PBMCs)
photodynamic diagnosis
photodynamic therapy (PDT)
protoporphyrin IX (PpIX)
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
21 Jan 2022
21 Jan 2022
Historique:
received:
29
12
2021
revised:
17
01
2022
accepted:
19
01
2022
entrez:
25
2
2022
pubmed:
26
2
2022
medline:
26
2
2022
Statut:
epublish
Résumé
Photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA), a precursor to the potent photosensitizer, protoporphyrin IX (PpIX), is an established modality for several malignant and premalignant diseases. This treatment is based on the light-activated PpIX in targeted lesions. Although numerous studies have confirmed the necrosis and apoptosis involved in the mechanism of action of this modality, little information is available for the change of exosome levels after treatment. We report from the first study on the effects of ALA-PDT on cytokines and exosomes of human healthy peripheral blood mononuclear cells (PBMCs). The treatment reduced the cytokines and exosomes studied, although there was variation among individual PBMC samples. This reduction is consistent with PDT-mediated survivals of subsets of PBMCs. More specifically, the ALA-PDT treatment apparently decreased all pro-inflammatory cytokines included, suggesting that this treatment may provide a strong anti-inflammatory effect. In addition, the treatment has decreased the levels of different types of exosomes, the HLA-DRDPDQ exosome in particular, which plays an important role in the rejection of organ transplantation as well as autoimmune diseases. These results may suggest future therapeutic strategies of ALA-PDT.
Identifiants
pubmed: 35203441
pii: biomedicines10020232
doi: 10.3390/biomedicines10020232
pmc: PMC8869139
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Southern and Eastern Norway Regional Health Authority
ID : 2016092
Organisme : Southern and Eastern Norway Regional Health Authority
ID : 2017058
Organisme : Southern and Eastern Norway Regional Health Authority
ID : 2020069
Organisme : Norwegian Radium Hospital Research Foundation
ID : SE1701
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