Promotion of cutaneous diabetic wound healing by subcutaneous administration of Wharton's jelly mesenchymal stem cells derived from umbilical cord.
Cell therapy
Diabetic wound
Subcutaneous administration
Wharton jelly mesenchymal stem cells
Journal
Archives of dermatological research
ISSN: 1432-069X
Titre abrégé: Arch Dermatol Res
Pays: Germany
ID NLM: 8000462
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
received:
23
08
2021
accepted:
30
11
2021
revised:
25
11
2021
pubmed:
8
2
2022
medline:
25
2
2023
entrez:
7
2
2022
Statut:
ppublish
Résumé
Wound healing is a major problem in diabetic patients, and current treatments have been confronted with limited success. The present study examined the benefit of Wharton's jelly mesenchymal stem cells (WJ-MSCs) derived from the human umbilical cord (UC) in wound healing in diabetic rats. Thirty days after inducing diabetes, a circular excision was created in the skin of rats, and the treatments were performed for 21 days. Two groups were studied, which included the Control group and WJ-MSCs group. The studied groups were sampled on the 7th, 14th, and 21st days after wounding. Histological ultrasound imaging of dermis and epidermis in the wound area for thickness and density measurement and skin elasticity were evaluated. Our results on post-wounding days 7, 14, and 21 showed that the wound closure, thickness, and density of new epidermis and dermis, as well as skin elasticity in the healed wound, were significantly higher in the WJ-MSCs group compared to the Control group. Subcutaneous administration of WJ-MSCs in diabetic wounds can effectively accelerate healing. Based on this, these cells can be used along with other treatment methods in the healing of different types of chronic wounds.
Identifiants
pubmed: 35129662
doi: 10.1007/s00403-022-02326-2
pii: 10.1007/s00403-022-02326-2
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
147-159Subventions
Organisme : Tehran University of Medical Sciences and Health Services
ID : 98/11626
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Ramachandran A, Snehalatha C, Shetty AS, Nanditha A (2012) Trends in prevalence of diabetes in Asian countries. World J Diabetes 3(6):110–117
pubmed: 22737281
pmcid: 3382707
doi: 10.4239/wjd.v3.i6.110
Bondor CI, Veresiu IA, Florea B, Vinik EJ, Vinik AI, Gavan NA (2016) Epidemiology of diabetic foot ulcers and amputations in romania: results of a cross-sectional quality of life questionnaire based survey. J Diabetes Res 2016:5439521
pubmed: 27019852
pmcid: 4785267
doi: 10.1155/2016/5439521
Whiting DR, Guariguata L, Weil C, Shaw J (2011) IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030. Diabetes Res Clin Pract 94(3):311–321
pubmed: 22079683
doi: 10.1016/j.diabres.2011.10.029
Qing C (2017) The molecular biology in wound healing & non-healing wound. Chinese J Traumatol 20(4):189–193
doi: 10.1016/j.cjtee.2017.06.001
Nasiry D, Khalatbary AR, Abdollahifar MA, Amini A, Bayat M, Noori A et al (2021) Engraftment of bioengineered three-dimensional scaffold from human amniotic membrane-derived extracellular matrix accelerates ischemic diabetic wound healing. Arch Dermatol Res 313(7):567–582
pubmed: 32940766
doi: 10.1007/s00403-020-02137-3
Frykberg RG, Banks J (2015) Challenges in the treatment of chronic wounds. Adv Wound Care 4(9):560–582
doi: 10.1089/wound.2015.0635
Jeffcoate WJ, Price PE, Phillips CJ, Game FL, Mudge E, Davies S et al (2009) Randomised controlled trial of the use of three dressing preparations in the management of chronic ulceration of the foot in diabetes. Health Technol Assessm (Winchester, England). 13(54):1–86
Greaves NS, Iqbal SA, Baguneid M, Bayat A (2013) The role of skin substitutes in the management of chronic cutaneous wounds. Wound Repair Regener 21(2):194–210
doi: 10.1111/wrr.12029
Moura LI, Dias AM, Carvalho E, de Sousa HC (2013) Recent advances on the development of wound dressings for diabetic foot ulcer treatment–a review. Acta Biomater 9(7):7093–7114
pubmed: 23542233
doi: 10.1016/j.actbio.2013.03.033
Perez-Favila A, Martinez-Fierro ML, Rodriguez-Lazalde JG, Cid-Baez MA, Zamudio-Osuna MJ, Martinez-Blanco MDR et al (2019) Current therapeutic strategies in diabetic foot ulcers. Medicina (Kaunas) 55(11):714
pubmed: 31731539
doi: 10.3390/medicina55110714
Wu Q, Chen B, Liang Z (2016) Mesenchymal stem cells as a prospective therapy for the diabetic foot. Stem Cells Int 2016:4612167
pubmed: 27867398
pmcid: 5102750
doi: 10.1155/2016/4612167
Han Y, Li X, Zhang Y, Han Y, Chang F, Ding J (2019) Mesenchymal stem cells for regenerative medicine. Cells 8(8):886
pubmed: 31412678
pmcid: 6721852
doi: 10.3390/cells8080886
Rohban R, Pieber TR (2017) Mesenchymal stem and progenitor cells in regeneration: tissue specificity and regenerative potential. Stem Cells Int 2017:5173732
pubmed: 28286525
pmcid: 5327785
doi: 10.1155/2017/5173732
Abdollahifar MA, Azad N, Faraji Sani M, Raoofi A, Abdi S, Aliaghaei A et al (2021) (2021) Impaired spermatogenesis caused by busulfan is partially ameliorated by treatment with conditioned medium of adipose tissue derived mesenchymal stem cells. Biotechnic Histochem. https://doi.org/10.1080/105202951905182
doi: 10.1080/105202951905182
Khosrotehrani K (2013) Mesenchymal stem cell therapy in skin: why and what for? Exp Dermatol 22(5):307–310
pubmed: 23614735
doi: 10.1111/exd.12141
Liu S, Yuan M, Hou K, Zhang L, Zheng X, Zhao B et al (2012) Immune characterization of mesenchymal stem cells in human umbilical cord Wharton’s jelly and derived cartilage cells. Cell Immunol 278(1–2):35–44
pubmed: 23121974
doi: 10.1016/j.cellimm.2012.06.010
Nekanti U, Rao VB, Bahirvani AG, Jan M, Totey S, Ta M (2010) Long-term expansion and pluripotent marker array analysis of Wharton’s jelly-derived mesenchymal stem cells. Stem Cells Developm 19(1):117–130
doi: 10.1089/scd.2009.0177
Fong CY, Chak LL, Biswas A, Tan JH, Gauthaman K, Chan WK et al (2011) Human Wharton’s jelly stem cells have unique transcriptome profiles compared to human embryonic stem cells and other mesenchymal stem cells. Stem Cell Rev Rep 7(1):1–16
pubmed: 20602182
doi: 10.1007/s12015-010-9166-x
Choi M, Lee HS, Naidansaren P, Kim HK, Cha JH et al (2013) Proangiogenic features of Wharton’s jelly-derived mesenchymal stromal/stem cells and their ability to form functional vessels. Int J Biochem Cell Biol 45(3):560–570
pubmed: 23246593
doi: 10.1016/j.biocel.2012.12.001
Dash SN, Dash NR, Guru B, Mohapatra PC (2014) Towards reaching the target: clinical application of mesenchymal stem cells for diabetic foot ulcers. Rejuvenation Res 17(1):40–53
pubmed: 24237303
doi: 10.1089/rej.2013.1467
Zhang S, Chen L, Zhang G, Zhang B (2020) Umbilical cord-matrix stem cells induce the functional restoration of vascular endothelial cells and enhance skin wound healing in diabetic mice via the polarized macrophages. Stem Cell Res Ther 11(1):1–15
Raoofi A, Sadeghi Y, Piryaei A, Sajadi E, Aliaghaei A, Rashidiani-Rashidabadi A et al (2021) Bone marrow mesenchymal stem cell condition medium loaded on PCL nanofibrous scaffold promoted nerve regeneration after sciatic nerve transection in male rats. Neurotox Res 39(5):1470–1486
pubmed: 34309780
doi: 10.1007/s12640-021-00391-5
Kouhbananinejad SM, Derakhshani A, Vahidi R, Dabiri S, Fatemi A, Armin F et al (2019) A fibrinous and allogeneic fibroblast-enriched membrane as a biocompatible material can improve diabetic wound healing. Biomater Sci 7(5):1949–1961
pubmed: 30793722
doi: 10.1039/C8BM01377B
Jalalie L, Rezaee MA, Rezaie MJ, Jalili A, Raoofi A, Rustamzade A (2021) Human umbilical cord mesenchymal stem cells improve morphometric and histopathologic changes of cyclophosphamide-injured ovarian follicles in mouse model of premature ovarian failure. Acta Histochem 123(1):151658
pubmed: 33249312
doi: 10.1016/j.acthis.2020.151658
Seyed Sharifi SH, Nasiry D, Mahmoudi F, Etezadpour M, Ebrahimzadeh MA (2021) Evaluation of sambucus ebulus fruit extract in full-thickness diabetic wound healing in rats. Jf Mazandaran Univer Med Sci 31(200):11–25
Nasiry D, Khalatbary AR, Ebrahimzadeh MA (2017) Anti-inflammatory and wound-healing potential of golden chanterelle mushroom, Cantharellus cibarius (Agaricomycetes). Int J Med Mushrooms 19(10):893–903
pubmed: 29256843
doi: 10.1615/IntJMedMushrooms.2017024674
Jackson WM, Nesti LJ, Tuan RS (2012) Concise review: clinical translation of wound healing therapies based on mesenchymal stem cells. Stem Cells Transl Med 1(1):44–50
pubmed: 23197639
doi: 10.5966/sctm.2011-0024
Fong CY, Tam K, Cheyyatraivendran S, Gan SU, Gauthaman K, Armugam A et al (2014) Human Wharton’s jelly stem cells and its conditioned medium enhance healing of excisional and diabetic wounds. J Cell Biochem 115(2):290–302
pubmed: 24038311
doi: 10.1002/jcb.24661
Montanucci P, di Pasquali C, Ferri I, Pescara T, Pennoni I, Siccu P et al (2017) Human umbilical cord wharton jelly-derived adult mesenchymal stem cells, in biohybrid scaffolds, for experimental skin regeneration. Stem cells international 2017:1472642
pubmed: 29456556
pmcid: 5804405
doi: 10.1155/2017/1472642
Yavari K, Abolhassani S, Mohammadnejad J (2016) Human umbilical cord blood stem cells differentiate into keratinocytes under in vitro conditions and culturing dif-ferentiated cells on bacterial cellulose film. Int J Stem Cell Res Transplant 4(7):216–219
Shokrgozar MA, Fattahi M, Bonakdar S, Ragerdi Kashani I, Majidi M, Haghighipour N et al (2012) Healing potential of mesenchymal stem cells cultured on a collagen-based scaffold for skin regeneration. Iran Biomed J 16(2):68–76
pubmed: 22801279
pmcid: 3600958
Ghaneialvar H, Arjmand S, Sahebghadam Lotfi A, Soleimani M, Mashhadi AF (2017) Influence of adipose derived mesenchymal stem cells on the effective inflammatory factors of diabetic wound healing in animal models. J Mazandaran Univer Med Sci 27(148):12–21
Millán-Rivero JE, Martínez CM, Romecín PA, Aznar-Cervantes SD, Carpes-Ruiz M, Cenis JL et al (2019) Silk fibroin scaffolds seeded with Wharton’s jelly mesenchymal stem cells enhance re-epithelialization and reduce formation of scar tissue after cutaneous wound healing. Stem Cell Res Ther 10(1):126
pubmed: 31029166
pmcid: 6487033
doi: 10.1186/s13287-019-1229-6
Hocking AM, Gibran NS (2010) Mesenchymal stem cells: paracrine signaling and differentiation during cutaneous wound repair. Exp Cell Res 316(14):2213–2219
pubmed: 20471978
pmcid: 2902653
doi: 10.1016/j.yexcr.2010.05.009
Duff M, Demidova O, Blackburn S, Shubrook J (2015) Cutaneous manifestations of diabetes mellitus. Clin Diabet Public Am Diabet Assoc 33(1):40–48
Argyropoulos AJ, Robichaud P, Balimunkwe RM, Fisher GJ, Hammerberg C, Yan Y et al (2016) Alterations of Dermal connective tissue collagen in diabetes: molecular basis of aged-appearing skin. PLoS ONE 11(4):e0153806
pubmed: 27104752
pmcid: 4841569
doi: 10.1371/journal.pone.0153806
Regulski MJ (2017) Mesenchymal stem cells: “guardians of inflammation.” Wounds Compend Clin Res Pract 29(1):20–27
Godwin J, Kuraitis D, Rosenthal N (2014) Extracellular matrix considerations for scar-free repair and regeneration: insights from regenerative diversity among vertebrates. Int J Biochem Cell Biol 56:47–55
pubmed: 25450455
doi: 10.1016/j.biocel.2014.10.011
Jung JA, Yoon YD, Lee HW, Kang SR, Han SK (2018) Comparison of human umbilical cord blood-derived mesenchymal stem cells with healthy fibroblasts on wound-healing activity of diabetic fibroblasts. Int Wound J 15(1):133–139
pubmed: 29115054
doi: 10.1111/iwj.12849
Azari O, Babaei H, Molaei M, Nematollahi-Mahani S, Layasi S (2008) The use of Wharton’s jelly-derived mesenchymal stem cells to accelerate second-intention cutaneous wound healing in goat. Iranian J Veterin Surg 3(3):15–27
Zhang S, Chen L, Zhang G, Zhang B (2020) Umbilical cord-matrix stem cells induce the functional restoration of vascular endothelial cells and enhance skin wound healing in diabetic mice via the polarized macrophages. Stem Cell Res Ther 11(1):39
pubmed: 31992364
pmcid: 6986138
doi: 10.1186/s13287-020-1561-x
Pashoutan Sarvar D, Shamsasenjan K, Akbarzadehlaleh P, Movassaghpour A, Timari H, Aqmasheh S (2017) The application of Mesenchymal stem cell-derived vesicles in regenerative medicine. Scient J Iran Blood Transfus Organ 14(3):237–248
Jonidi Shariatzadeh F, Gheydari K, Solouk A, Bonakdar S (2018) Use of stem cells in cartilage tissue regeneration and engineering: a review. Pathobiol Res 21(1):41–63
Li F, Huang Q, Chen J, Peng Y, Roop DR, Bedford JS et al (2010) Apoptotic cells activate the “phoenix rising” pathway to promote wound healing and tissue regeneration. Sci Signal 3(110):ra13
pubmed: 20179271
pmcid: 2905599
doi: 10.1126/scisignal.2000634
McAndrews KM, McGrail DJ, Ravikumar N, Dawson MR (2015) Mesenchymal stem cells induce directional migration of invasive breast cancer cells through TGF-β. Sci Rep 5:16941
pubmed: 26585689
pmcid: 4653660
doi: 10.1038/srep16941