A New Strategy to Preserve and Assess Oxygen Consumption in Murine Tissues.
Aging
/ physiology
Animals
Cell Communication
/ physiology
Cell Line
Cell Line, Tumor
Cell Respiration
/ physiology
Energy Metabolism
/ physiology
Heart
/ physiology
Humans
Lung
/ physiology
Male
Mice
Mice, Inbred C57BL
Mitochondria
/ physiology
Mitochondrial Membranes
/ physiology
Oxygen Consumption
/ physiology
Rats
Respiratory Function Tests
/ methods
energy metabolism
high fat diet
oxidative phosphorylation
oximetry
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
22 Dec 2021
22 Dec 2021
Historique:
received:
08
11
2021
revised:
13
12
2021
accepted:
17
12
2021
entrez:
11
1
2022
pubmed:
12
1
2022
medline:
1
2
2022
Statut:
epublish
Résumé
Mitochondrial dysfunctions are implicated in several pathologies, such as metabolic, cardiovascular, respiratory, and neurological diseases, as well as in cancer and aging. These metabolic alterations are usually assessed in human or murine samples by mitochondrial respiratory chain enzymatic assays, by measuring the oxygen consumption of intact mitochondria isolated from tissues, or from cells obtained after physical or enzymatic disruption of the tissues. However, these methodologies do not maintain tissue multicellular organization and cell-cell interactions, known to influence mitochondrial metabolism. Here, we develop an optimal model to measure mitochondrial oxygen consumption in heart and lung tissue samples using the XF24 Extracellular Flux Analyzer (Seahorse) and discuss the advantages and limitations of this technological approach. Our results demonstrate that tissue organization, as well as mitochondrial ultrastructure and respiratory function, are preserved in heart and lung tissues freshly processed or after overnight conservation at 4 °C. Using this method, we confirmed the repeatedly reported obesity-associated mitochondrial dysfunction in the heart and extended it to the lungs. We set up and validated a new strategy to optimally assess mitochondrial function in murine tissues. As such, this method is of great potential interest for monitoring mitochondrial function in cohort samples.
Identifiants
pubmed: 35008535
pii: ijms23010109
doi: 10.3390/ijms23010109
pmc: PMC8745047
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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