Neural crest-derived cells possess differentiation potential to keratinocytes in the process of wound healing.

Animals Cell Differentiation / physiology Keratinocytes / cytology Mice Mice, Transgenic Mouth Mucosa / metabolism Neural Crest / cytology Osteoblasts / cytology Palate / cytology Wound Healing / physiology
Keratinocyte Neural crest-derived cell Osteoblast Palate mucosa Palate wound healing

Journal

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Titre abrégé: Biomed Pharmacother
Pays: France
ID NLM: 8213295

Informations de publication

Date de publication:
Feb 2022
Historique:
received: 10 12 2021
revised: 23 12 2021
accepted: 23 12 2021
pubmed: 31 12 2021
medline: 22 3 2022
entrez: 30 12 2021
Statut: ppublish

Résumé

Neural crest-derived cells (NCDCs), which exist as neural crest cells during the fetal stage and differentiate into palate cells, also exist in adult palate tissues, though with unknown roles. In the present study, NCDCs were labeled with EGFP derived from P0-Cre/CAG-CAT-EGFP (P0-EGFP) double transgenic mice, then their function in palate mucosa wound healing was analyzed. As a palate wound healing model, left-side palate mucosa of P0-EGFP mice was resected, and stem cell markers and keratinocyte markers were detected in healed areas. NCDCs were extracted from normal palate mucosa and precultured with stem cell media for 14 days, then were differentiated into keratinocytes or osteoblasts to analyze pluripotency. The wound healing process started with marginal mucosal regeneration on day two and the entire wound area was lined by regenerated mucosa with EGFP-positive cells (NCDCs) on day 28. EGFP-positive cells comprised approximately 60% of cells in healed oral mucosa, and 65% of those expressed stem cell markers (Sca-1

Identifiants

DOI: 10.1016/j.biopha.2021.112593 PMID: 34968925
pubmed: 34968925
pii: S0753-3322(21)01380-9
doi: 10.1016/j.biopha.2021.112593
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

112593

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Auteurs

Hideomi Takizawa (H)

Department of Orthodontics, Showa University School of Dentistry, 2-1-1 Kitasenzoku, Ota-ku, Tokyo 145-8515, Japan; Department of Pharmacology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Pharmacological Research Center, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

Akiko Karakawa (A)

Department of Pharmacology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Pharmacological Research Center, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. Electronic address: a-karakawa@dent.showa-u.ac.jp.

Tetsuo Suzawa (T)

Department of Biochemistry, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

Masahiro Chatani (M)

Department of Pharmacology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Pharmacological Research Center, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

Megumi Ikeda (M)

Department of Pharmacology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Pharmacological Research Center, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Division of Endodontology, Department of Conservative Dentistry, Showa University School of Dentistry, 2-1-1 Kitasenzoku, Ota-ku, Tokyo, 145-8515, Japan.

Nobuhiro Sakai (N)

Department of Pharmacology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Pharmacological Research Center, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

Yuki Azetsu (Y)

Department of Pharmacology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Pharmacological Research Center, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

Masahiro Takahashi (M)

Department of Orthodontics, Showa University School of Dentistry, 2-1-1 Kitasenzoku, Ota-ku, Tokyo 145-8515, Japan.

Eri Urano (E)

Department of Prosthodontics, Showa University School of Dentistry, 2-1-1 Kitasenzoku, Ota-ku, Tokyo 145-8515, Japan.

Ryutaro Kamijo (R)

Department of Biochemistry, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

Koutaro Maki (K)

Department of Orthodontics, Showa University School of Dentistry, 2-1-1 Kitasenzoku, Ota-ku, Tokyo 145-8515, Japan.

Masamichi Takami (M)

Department of Pharmacology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Pharmacological Research Center, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. Electronic address: takami@dent.showa-u.ac.jp.

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Classifications MeSH

Recherchemedicale.com › Eucaryotes › Animaux › Neural crest-derived cells possess...
Recherchemedicale.com › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Neural crest-derived cells possess...
Recherchemedicale.com › Cellules › Cellules épithéliales › Kératinocytes › Neural crest-derived cells possess...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Neural crest-derived cells possess...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › Neural crest-derived cells possess...
Recherchemedicale.com › Système stomatognathique › Bouche › Muqueuse de la bouche › Neural crest-derived cells possess...
Recherchemedicale.com › Structures de l'embryon › Crête neurale › Neural crest-derived cells possess...
Recherchemedicale.com › Cellules › Cellules du tissu conjonctif › Ostéoblastes › Neural crest-derived cells possess...
Recherchemedicale.com › Système stomatognathique › Bouche › Palais › Neural crest-derived cells possess...
Recherchemedicale.com › Phénomènes biologiques › Régénération › Cicatrisation de plaie › Neural crest-derived cells possess...