Genome-wide distribution of 5hmC in the dental pulp of mouse molars and incisors.

Animals Cell Differentiation Dental Pulp Genome Incisor Mice Odontoblasts
5-hydroxymethylcysteine (5hmC) TET enzymes dental pulp gene body promoter

Journal

Journal of biochemistry
ISSN: 1756-2651
Titre abrégé: J Biochem
Pays: England
ID NLM: 0376600

Informations de publication

Date de publication:
07 Jan 2022
Historique:
received: 15 07 2021
accepted: 13 10 2021
pubmed: 23 10 2021
medline: 11 2 2022
entrez: 22 10 2021
Statut: ppublish

Résumé

The dental pulp is critical for the production of odontoblasts to create reparative dentin. In recent years, dental pulp has become a promising source of mesenchymal stem cells that are capable of differentiating into multiple cell types. To elucidate the transcriptional control mechanisms specifying the early phases of odontoblast differentiation, we analysed the DNA demethylation pattern associated with 5-hydroxymethylcytosine (5hmC) in the primary murine dental pulp. 5hmC plays an important role in chromatin accessibility and transcriptional control by modelling a dynamic equilibrium between DNA methylation and demethylation. Our research revealed 5hmC enrichment along genes and non-coding regulatory regions associated with specific developmental pathways in the genome of mouse incisor and molar dental pulp. Although the overall distribution of 5hmC is similar, the intensity and location of the 5hmC peaks significantly differs between the incisor and molar pulp genome, indicating cell type-specific epigenetic variations. Our study suggests that the differential DNA demethylation pattern could account for the distinct regulatory mechanisms underlying the tooth-specific ontogenetic programs.

Identifiants

DOI: 10.1093/jb/mvab114 PMID: 34676418 PMC: PMC8900285
pubmed: 34676418
pii: 6407970
doi: 10.1093/jb/mvab114
pmc: PMC8900285
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

123-129

Subventions

Organisme : OVPR
ID : R01-DE016689
Organisme : Department of Computer Science and Engineering, University of Connecticut
Organisme : NIDCR NIH HHS
ID : R01 DE016689
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD098636
Pays : United States
Organisme : NIH HHS
ID : HD098636
Pays : United States

Commentaires et corrections

Type : ErratumIn

Informations de copyright

© The Author(s) 2021. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

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Auteurs

Pujan Joshi (P)

Computer Science and Engineering Department, University of Connecticut, 371 Fairfield Way, Unit 4155, Storrs, CT 06269, USA.

Anushree Vijaykumar (A)

Department of Craniofacial Sciences, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA.

Badam Enkhmandakh (B)

Center for Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA.

Mina Mina (M)

Department of Craniofacial Sciences, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA.

Dong-Guk Shin (DG)

Computer Science and Engineering Department, University of Connecticut, 371 Fairfield Way, Unit 4155, Storrs, CT 06269, USA.

Dashzeveg Bayarsaihan (D)

Institute for System Genomics and Center for Regenerative Medicine and Skeletal Development, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA.

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Classifications MeSH

Recherchemedicale.com › Eucaryotes › Animaux › Genome-wide distribution of 5hmC in the dental...
Recherchemedicale.com › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Genome-wide distribution of 5hmC in the dental...
Recherchemedicale.com › Système stomatognathique › Bouche › Denture › Composants dentaires › Pulpe dentaire › Genome-wide distribution of 5hmC in the dental...
Recherchemedicale.com › Phénomènes génétiques › Structures génétiques › Génome › Genome-wide distribution of 5hmC in the dental...
Recherchemedicale.com › Système stomatognathique › Bouche › Denture › Dent › Incisive › Genome-wide distribution of 5hmC in the dental...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Genome-wide distribution of 5hmC in the dental...
Recherchemedicale.com › Cellules › Cellules du tissu conjonctif › Odontoblastes › Genome-wide distribution of 5hmC in the dental...