Red blood cells and their releasates compromise bone marrow-derived human mesenchymal stem/stromal cell survival in vitro.
Apoptosis
Bone marrow aspirate concentrate
Hematocrit
Mesenchymal stem cells
Necrosis
Red blood cells
Viability
Journal
Stem cell research & therapy
ISSN: 1757-6512
Titre abrégé: Stem Cell Res Ther
Pays: England
ID NLM: 101527581
Informations de publication
Date de publication:
21 10 2021
21 10 2021
Historique:
received:
19
05
2021
accepted:
18
08
2021
entrez:
22
10
2021
pubmed:
23
10
2021
medline:
30
10
2021
Statut:
epublish
Résumé
The use of bone marrow aspirate (BMA) and bone marrow aspirate concentrate (BMC) in the treatment of inflammatory orthopedic conditions has become a common practice. The therapeutic effect of BMA/BMC is thought to revolve primarily around the mesenchymal stem/stromal cell (MSC) population residing within the nucleated cell fraction. MSCs have the unique ability to respond to site of injury via the secretion of immunomodulating factors, resolving inflammation in diseased joints. Recently, the importance of hematocrit (HCT) in BMC has been debated, as the potential impact on MSC function is unknown. In the present study, we investigate MSC health over a short time-course following exposure to a range of HCT and red blood cell releasate (RBC Bone marrow-derived human MSCs in early passage were grown under conditions of 0%, 2.5%, 5%, 10%, 20% and 40% HCT and RBC Significant reductions in viable MSCs concurrent with an increase in necrotic MSCs in high HCT and RBC Various levels of HCT and RBC
Identifiants
pubmed: 34674751
doi: 10.1186/s13287-021-02610-4
pii: 10.1186/s13287-021-02610-4
pmc: PMC8529765
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
547Informations de copyright
© 2021. The Author(s).
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