Phagosome resolution regenerates lysosomes and maintains the degradative capacity in phagocytes.
Actin Cytoskeleton
/ metabolism
Actins
/ genetics
Animals
Clathrin
/ genetics
Escherichia coli
/ chemistry
Gene Expression
Genes, Reporter
Green Fluorescent Proteins
/ genetics
Lysosomes
/ metabolism
Membrane Fusion
Mice
Microtubules
/ metabolism
Phagocytosis
/ physiology
Phagosomes
/ metabolism
Proteolysis
RAW 264.7 Cells
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
06 09 2021
06 09 2021
Historique:
received:
14
05
2020
revised:
05
05
2021
accepted:
14
06
2021
entrez:
28
6
2021
pubmed:
29
6
2021
medline:
10
11
2021
Statut:
ppublish
Résumé
Phagocytes engulf unwanted particles into phagosomes that then fuse with lysosomes to degrade the enclosed particles. Ultimately, phagosomes must be recycled to help recover membrane resources that were consumed during phagocytosis and phagosome maturation, a process referred to as "phagosome resolution." Little is known about phagosome resolution, which may proceed through exocytosis or membrane fission. Here, we show that bacteria-containing phagolysosomes in macrophages undergo fragmentation through vesicle budding, tubulation, and constriction. Phagosome fragmentation requires cargo degradation, the actin and microtubule cytoskeletons, and clathrin. We provide evidence that lysosome reformation occurs during phagosome resolution since the majority of phagosome-derived vesicles displayed lysosomal properties. Importantly, we show that clathrin-dependent phagosome resolution is important to maintain the degradative capacity of macrophages challenged with two waves of phagocytosis. Overall, our work suggests that phagosome resolution contributes to lysosome recovery and to maintaining the degradative power of macrophages to handle multiple waves of phagocytosis.
Identifiants
pubmed: 34180943
pii: 212440
doi: 10.1083/jcb.202005072
pmc: PMC8241537
pii:
doi:
Substances chimiques
Actins
0
Clathrin
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : CIHR
ID : 123373
Pays : Canada
Informations de copyright
© 2021 Lancaster et al.
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