Polyclonal expansion of TCR Vbeta 21.3
Adult
CD4-Positive T-Lymphocytes
/ immunology
CD8-Positive T-Lymphocytes
/ immunology
COVID-19
/ immunology
Child
Child, Preschool
Cytokines
/ blood
HLA-DR Antigens
/ immunology
Humans
Lymphocyte Activation
/ immunology
Receptors, Antigen, T-Cell, alpha-beta
/ immunology
SARS-CoV-2
/ immunology
Systemic Inflammatory Response Syndrome
/ immunology
Journal
Science immunology
ISSN: 2470-9468
Titre abrégé: Sci Immunol
Pays: United States
ID NLM: 101688624
Informations de publication
Date de publication:
25 05 2021
25 05 2021
Historique:
received:
19
02
2021
accepted:
18
05
2021
entrez:
26
5
2021
pubmed:
27
5
2021
medline:
10
6
2021
Statut:
ppublish
Résumé
Multiple Inflammatory Syndrome in Children (MIS-C) is a delayed and severe complication of SARS-CoV-2 infection that strikes previously healthy children. As MIS-C combines clinical features of Kawasaki disease and Toxic Shock Syndrome (TSS), we aimed to compare the immunological profile of pediatric patients with these different conditions. We analyzed blood cytokine expression, and the T cell repertoire and phenotype in 36 MIS-C cases, which were compared to 16 KD, 58 TSS, and 42 COVID-19 cases. We observed an increase of serum inflammatory cytokines (IL-6, IL-10, IL-18, TNF-α, IFNγ, CD25s, MCP1, IL-1RA) in MIS-C, TSS and KD, contrasting with low expression of HLA-DR in monocytes. We detected a specific expansion of activated T cells expressing the Vβ21.3 T cell receptor β chain variable region in both CD4 and CD8 subsets in 75% of MIS-C patients and not in any patient with TSS, KD, or acute COVID-19; this correlated with the cytokine storm detected. The T cell repertoire returned to baseline within weeks after MIS-C resolution. Vβ21.3+ T cells from MIS-C patients expressed high levels of HLA-DR, CD38 and CX3CR1 but had weak responses to SARS-CoV-2 peptides in vitro
Identifiants
pubmed: 34035116
pii: 6/59/eabh1516
doi: 10.1126/sciimmunol.abh1516
pmc: PMC8815705
mid: NIHMS1770032
pii:
doi:
Substances chimiques
Cytokines
0
HLA-DR Antigens
0
Receptors, Antigen, T-Cell, alpha-beta
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
Copyright © 2021, American Association for the Advancement of Science.
Références
Nat Commun. 2020 Oct 21;11(1):5341
pubmed: 33087723
Nat Med. 2021 Mar;27(3):454-462
pubmed: 33589825
Immunotargets Ther. 2020 Nov 04;9:241-253
pubmed: 33178636
Lancet. 2020 May 23;395(10237):1607-1608
pubmed: 32386565
Nat Methods. 2015 May;12(5):380-1
pubmed: 25924071
J Clin Invest. 2021 May 17;131(10):
pubmed: 33705359
J Allergy Clin Immunol. 2020 Nov;146(5):1194-1200.e1
pubmed: 32853638
J Exp Med. 1990 Sep 1;172(3):981-4
pubmed: 2117641
Science. 1990 May 11;248(4956):685-6
pubmed: 2333520
Arthritis Rheum. 2004 Feb;50(2):589-97
pubmed: 14872503
Lancet Neurol. 2013 Dec;12(12):1159-69
pubmed: 24183309
J Clin Invest. 2020 Nov 2;130(11):5967-5975
pubmed: 32730233
N Engl J Med. 2020 Jul 23;383(4):334-346
pubmed: 32598831
J Infect Dis. 2005 Feb 15;191(4):499-502
pubmed: 15655771
Cytometry B Clin Cytom. 2013 Jan-Feb;84(1):59-62
pubmed: 22987669
Proc Natl Acad Sci U S A. 2020 Oct 13;117(41):25254-25262
pubmed: 32989130
Cell. 2020 Jun 11;181(6):1194-1199
pubmed: 32405102
J Clin Immunol. 1995 Nov;15(6 Suppl):11S-17S
pubmed: 8613483
Cytokine. 2019 Jan;113:446-452
pubmed: 30413290
N Engl J Med. 2020 Oct 29;383(18):1793-1794
pubmed: 33085852
Cytometry B Clin Cytom. 2017 Nov;92(6):456-464
pubmed: 26804473
Clin Chem. 2002 Sep;48(9):1589-92
pubmed: 12194941
J Allergy Clin Immunol. 2020 Jul;146(1):206-208.e2
pubmed: 32360285
Cell. 2020 Nov 12;183(4):968-981.e7
pubmed: 32966765
N Engl J Med. 2020 Jul 23;383(4):393-395
pubmed: 32598829
Bioinformatics. 2014 Oct;30(19):2811-2
pubmed: 24930139
Science. 2020 Oct 23;370(6515):
pubmed: 32972996
Bioinformatics. 2002 Feb;18(2):329-30
pubmed: 11847085
Sci Immunol. 2021 Mar 2;6(57):
pubmed: 33653907
Lancet. 2020 Jun 6;395(10239):1771-1778
pubmed: 32410760
Immunity. 2021 May 11;54(5):1083-1095.e7
pubmed: 33891889
Science. 1989 May 19;244(4906):811-3
pubmed: 2524876
JAMA. 2021 Mar 2;325(9):855-864
pubmed: 33523115
Infect Immun. 2009 May;77(5):2043-50
pubmed: 19255190
Curr Opin Immunol. 2013 Aug;25(4):477-83
pubmed: 23725873
Crit Care. 2018 Sep 11;22(1):213
pubmed: 30205835
N Engl J Med. 2020 Jul 23;383(4):347-358
pubmed: 32598830
Nat Med. 2020 Nov;26(11):1701-1707
pubmed: 32812012
Cell. 1989 Jan 13;56(1):27-35
pubmed: 2521300
Cell. 2020 Nov 12;183(4):982-995.e14
pubmed: 32991843
Front Pediatr. 2020 Dec 04;8:605807
pubmed: 33344389
J Clin Invest. 2021 Mar 15;131(6):
pubmed: 33497356
Euro Surveill. 2020 Jun;25(22):
pubmed: 32524957
JAMA. 1984 Dec 28;252(24):3399-402
pubmed: 6389917
Science. 2020 Aug 7;369(6504):718-724
pubmed: 32661059
J Exp Med. 2021 Jun 7;218(6):
pubmed: 33904890