Bioprinting and Differentiation of Adipose-Derived Stromal Cell Spheroids for a 3D Breast Cancer-Adipose Tissue Model.
Adipogenesis
Adipose Tissue
/ cytology
Bioprinting
Breast Neoplasms
/ pathology
Cell Differentiation
Cell Survival
Extracellular Matrix
/ metabolism
Female
Humans
Hyaluronic Acid
/ chemistry
Hydrogels
/ chemistry
Models, Biological
Printing, Three-Dimensional
Spheroids, Cellular
/ cytology
Stromal Cells
/ cytology
adipose tissue
adipose-derived stromal cells
bioprinting
breast cancer model
extracellular matrix
hyaluronic acid
spheroids
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
03 04 2021
03 04 2021
Historique:
received:
28
02
2021
revised:
27
03
2021
accepted:
30
03
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
21
10
2021
Statut:
epublish
Résumé
Biofabrication, including printing technologies, has emerged as a powerful approach to the design of disease models, such as in cancer research. In breast cancer, adipose tissue has been acknowledged as an important part of the tumor microenvironment favoring tumor progression. Therefore, in this study, a 3D-printed breast cancer model for facilitating investigations into cancer cell-adipocyte interaction was developed. First, we focused on the printability of human adipose-derived stromal cell (ASC) spheroids in an extrusion-based bioprinting setup and the adipogenic differentiation within printed spheroids into adipose microtissues. The printing process was optimized in terms of spheroid viability and homogeneous spheroid distribution in a hyaluronic acid-based bioink. Adipogenic differentiation after printing was demonstrated by lipid accumulation, expression of adipogenic marker genes, and an adipogenic ECM profile. Subsequently, a breast cancer cell (MDA-MB-231) compartment was printed onto the adipose tissue constructs. After nine days of co-culture, we observed a cancer cell-induced reduction of the lipid content and a remodeling of the ECM within the adipose tissues, with increased fibronectin, collagen I and collagen VI expression. Together, our data demonstrate that 3D-printed breast cancer-adipose tissue models can recapitulate important aspects of the complex cell-cell and cell-matrix interplay within the tumor-stroma microenvironment.
Identifiants
pubmed: 33916870
pii: cells10040803
doi: 10.3390/cells10040803
pmc: PMC8066030
pii:
doi:
Substances chimiques
Hydrogels
0
Hyaluronic Acid
9004-61-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : 326998133
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