Expression of epidermal antimicrobial peptides is increased in tinea pedis.
Adult
Aged
Aged, 80 and over
Arthrodermataceae
/ immunology
Defensins
/ metabolism
Female
Humans
Immunity, Innate
Keratinocytes
/ metabolism
Male
Middle Aged
Pore Forming Cytotoxic Proteins
/ metabolism
Ribonucleases
/ metabolism
S100 Calcium Binding Protein A7
/ metabolism
Skin
/ metabolism
Skin Diseases, Infectious
/ immunology
Tinea Pedis
/ immunology
antimicrobial peptides
defensins
dermatophytes
infectious skin diseases
innate immunity
tinea pedis
Journal
Mycoses
ISSN: 1439-0507
Titre abrégé: Mycoses
Pays: Germany
ID NLM: 8805008
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
revised:
23
03
2021
received:
24
02
2021
accepted:
25
03
2021
pubmed:
3
4
2021
medline:
7
10
2021
entrez:
2
4
2021
Statut:
ppublish
Résumé
Tinea pedis is often chronic or recurrent, but not all individuals are equally susceptible to this infection. Dermatophytes are able to induce the expression of antimicrobial peptides and proteins (AMPs) in human keratinocytes and certain AMPs can inhibit the growth of dermatophytes. The focus of this study was to analyse the secretion of relevant AMPs, especially RNase 7, human beta-defensin-2 (hBD-2) and the S-100 protein psoriasin (S100A7), in patients with confirmed tinea pedis. To verify the diagnosis, skin scales were obtained from all patients (n = 13) and the dermatophytes were identified by potassium hydroxide mount, culture and molecular analysis. To determine the AMP concentrations, the lesional skin area of the foot was rinsed with a buffer that was subsequently analysed by ELISA. The corresponding area of the other unaffected foot as well as defined healthy skin areas of the forearm and forehead and samples from age and gender-matched healthy volunteers served as controls. In tinea pedis patients the AMP concentrations were higher in lesional skin than in non-lesional skin and in healthy skin of controls. In particular, concentrations of hBD-2 and psoriasin were significantly elevated. The induction of AMPs in tinea pedis might be triggered directly by the dermatophytes; furthermore, attendant inflammation and/or differentiation processes may play a role. Our results indicate that there is no defect in the constitutive expression and induction of the analysed AMPs by dermatophytes in the epidermis of affected patients. However, it is not known why the elevated AMP concentrations fail to efficiently combat dermatophyte growth.
Sections du résumé
BACKGROUND
BACKGROUND
Tinea pedis is often chronic or recurrent, but not all individuals are equally susceptible to this infection. Dermatophytes are able to induce the expression of antimicrobial peptides and proteins (AMPs) in human keratinocytes and certain AMPs can inhibit the growth of dermatophytes.
OBJECTIVE
OBJECTIVE
The focus of this study was to analyse the secretion of relevant AMPs, especially RNase 7, human beta-defensin-2 (hBD-2) and the S-100 protein psoriasin (S100A7), in patients with confirmed tinea pedis.
METHODS
METHODS
To verify the diagnosis, skin scales were obtained from all patients (n = 13) and the dermatophytes were identified by potassium hydroxide mount, culture and molecular analysis. To determine the AMP concentrations, the lesional skin area of the foot was rinsed with a buffer that was subsequently analysed by ELISA. The corresponding area of the other unaffected foot as well as defined healthy skin areas of the forearm and forehead and samples from age and gender-matched healthy volunteers served as controls.
RESULTS
RESULTS
In tinea pedis patients the AMP concentrations were higher in lesional skin than in non-lesional skin and in healthy skin of controls. In particular, concentrations of hBD-2 and psoriasin were significantly elevated.
CONCLUSIONS
CONCLUSIONS
The induction of AMPs in tinea pedis might be triggered directly by the dermatophytes; furthermore, attendant inflammation and/or differentiation processes may play a role. Our results indicate that there is no defect in the constitutive expression and induction of the analysed AMPs by dermatophytes in the epidermis of affected patients. However, it is not known why the elevated AMP concentrations fail to efficiently combat dermatophyte growth.
Substances chimiques
Defensins
0
Pore Forming Cytotoxic Proteins
0
S100 Calcium Binding Protein A7
0
Ribonucleases
EC 3.1.-
Ribonuclease 7
EC 3.1.27.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
763-770Informations de copyright
© 2021 The Authors. Mycoses published by Wiley-VCH GmbH.
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