Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients.
Adult
Aged
Elasticity Imaging Techniques
Fatty Liver
/ diagnostic imaging
Female
Gastrointestinal Microbiome
Gastrointestinal Tract
/ microbiology
Humans
Intra-Abdominal Fat
/ microbiology
Liver Cirrhosis
/ diagnostic imaging
Male
Middle Aged
Muscle, Skeletal
/ microbiology
Obesity
/ physiopathology
Prospective Studies
Young Adult
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
12 01 2021
12 01 2021
Historique:
received:
03
09
2020
accepted:
09
12
2020
entrez:
13
1
2021
pubmed:
14
1
2021
medline:
11
8
2021
Statut:
epublish
Résumé
Obesity could lead to metabolic dysfunction-associated fatty liver disease (MAFLD), which severity could be linked to muscle and gut microbiota disturbances. Our prospective study enrolled 52 obese patients whose MAFLD severity was estimated by transient elastography. Patients with severe steatosis (n = 36) had higher ALAT values, fasting blood glucose levels as well as higher visceral adipose tissue area and skeletal muscle index evaluated by computed tomography. Patients with fibrosis (n = 13) had higher ASAT values, increased whole muscle area and lower skeletal muscle density index. In a multivariate logistic regression analysis, myosteatosis was the strongest factor associated with fibrosis. Illumina sequencing of 16S rRNA gene amplicon was performed on fecal samples. The relative abundance of fecal Clostridium sensu stricto was significantly decreased with the presence of liver fibrosis and was negatively associated with liver stiffness measurement and myosteatosis. In addition, 19 amplicon sequence variants were regulated according to the severity of the disease. Linear discriminant analysis effect size (LEfSe) also highlighted discriminant microbes in patients with fibrosis, such as an enrichment of Enterobacteriaceae and Escherichia/Shigella compared to patients with severe steatosis without fibrosis. All those data suggest a gut-liver-muscle axis in the pathogenesis of MAFLD complications.
Identifiants
pubmed: 33436764
doi: 10.1038/s41598-020-79718-9
pii: 10.1038/s41598-020-79718-9
pmc: PMC7804131
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
659Commentaires et corrections
Type : ErratumIn
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