IFIT Proteins Are Involved in CXCL10 Expression in Human Glomerular Endothelial Cells Treated with a Toll-Like Receptor 3 Agonist.
Adaptor Proteins, Signal Transducing
/ biosynthesis
Apoptosis Regulatory Proteins
/ biosynthesis
Cells, Cultured
Chemokine CXCL10
/ biosynthesis
Dose-Response Relationship, Drug
Endothelial Cells
/ drug effects
Gene Expression
Humans
Intracellular Signaling Peptides and Proteins
/ biosynthesis
Kidney Glomerulus
/ cytology
Poly I-C
/ pharmacology
RNA-Binding Proteins
/ biosynthesis
Toll-Like Receptor 3
/ agonists
CXCL10
Glomerular endothelial cells
IFIT1
IFIT2
IFIT3
Journal
Kidney & blood pressure research
ISSN: 1423-0143
Titre abrégé: Kidney Blood Press Res
Pays: Switzerland
ID NLM: 9610505
Informations de publication
Date de publication:
2021
2021
Historique:
received:
04
08
2020
accepted:
24
09
2020
pubmed:
17
12
2020
medline:
11
3
2021
entrez:
16
12
2020
Statut:
ppublish
Résumé
Various viruses including a novel coronavirus (SARS-CoV-2) can infect the kidney. When viruses invade the glomeruli from the bloodstream, glomerular endothelial cells (GECs) initiate the innate immune reactions. We investigated the expression of interferon (IFN)-induced protein with tetratricopeptide repeats (IFIT) 1/2/3, antiviral molecules, in human GECs treated with a toll-like receptor (TLR) 3 agonist. Role of IFIT1/2/3 in the expression of C-X-C motif chemokine ligand 10 (CXCL10) was also examined. Human GECs were cultured and stimulated with polyinosinic-polycytidylic acid (poly IC), a synthetic TLR3 agonist. Real-time qPCR, Western blotting, and ELISA were used to examine the expression of IFIT1/2/3, IFN-β, and CXCL10. RNA interference against IFN-β or IFIT1/2/3 was also performed. Expression of IFIT1/2/3 and CXCL10 was induced by poly IC in GECs. The inductions were inhibited by RNA interfering of IFN-β. Knockdown of IFIT1/2/3 decreased the CXCL10 expression. Knockdown of IFIT3 decreased the expression of IFIT1 and IFIT2 proteins. IFIT1/2/3 and CXCL10 were induced by poly IC via IFN-β in GECs. IFIT1/2/3 may increase the expression of CXCL10 which induces lymphocyte chemotaxis and may inhibit the replication of infected viruses. These molecules may play a role in GEC innate immune reactions in response to viruses.
Identifiants
pubmed: 33326977
pii: 000511915
doi: 10.1159/000511915
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Apoptosis Regulatory Proteins
0
CXCL10 protein, human
0
Chemokine CXCL10
0
IFIT1 protein, human
0
IFIT2 protein, human
0
IFIT3 protein, human
0
Intracellular Signaling Peptides and Proteins
0
RNA-Binding Proteins
0
TLR3 protein, human
0
Toll-Like Receptor 3
0
Poly I-C
O84C90HH2L
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
74-83Informations de copyright
© 2020 The Author(s). Published by S. Karger AG, Basel.