Effects of maternal and fetal choline concentrations on the fetal growth and placental DNA methylation of 12 target genes related to fetal growth, adipogenesis, and energy metabolism.


Journal

The journal of obstetrics and gynaecology research
ISSN: 1447-0756
Titre abrégé: J Obstet Gynaecol Res
Pays: Australia
ID NLM: 9612761

Informations de publication

Date de publication:
Feb 2021
Historique:
received: 27 07 2020
revised: 17 11 2020
accepted: 30 11 2020
pubmed: 11 12 2020
medline: 22 6 2021
entrez: 10 12 2020
Statut: ppublish

Résumé

We performed a birth cohort study involving 124 mother-infant pairs to investigate whether placental DNA methylation is associated with maternal choline status and fetal development. Plasma choline concentration was assayed longitudinally in the 1st and 3rd trimesters and at term-pregnancy in mothers and cord blood. Placental DNA methylation was measured for 12 target candidate genes that are related to fetal growth, adipogenesis, lipid and energy metabolism, or long interspersed nuclear elements. Higher maternal plasma and cord blood choline levels at term tended to associate with lower birthweight (r = -0.246, P < 0.013; r = -0.290, P < 0.002) and body mass index (BMI) at birth (r = 0.344, P < 1E-3; r = -0.360, P < 1E-3). The correlation between maternal plasma choline level and cord blood choline level was relatively modest (r = 0.049, P = 0.639). There was an inverse correlation between placental DNA methylation at the retinoid X receptor alpha (RXRA) gene and maternal plasma choline level (r = -0.188 to r = -0.452, P = 0.043 to P < 1E-3 at three points). RXRA methylation level was positively associated with birthweight and BMI at birth (r = 0.306, P = 0.001; r = 0.390, P < 1E-3). Further, RXRA methylation was inversely correlated with RXRA gene expression level (r = 0.333, P < 1E-3). Our results suggest that the association between maternal choline status and placental RXRA methylation represents a potential fetal programing mechanism contributing to fetal growth.

Identifiants

pubmed: 33300271
doi: 10.1111/jog.14599
doi:

Substances chimiques

Choline N91BDP6H0X

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

734-744

Subventions

Organisme : JSPS KAKENHI
ID : JP15K16537
Organisme : JSPS KAKENHI
ID : JP17K13237
Organisme : NCGM Intramural Research Fund
ID : 23A103

Informations de copyright

© 2020 Japan Society of Obstetrics and Gynecology.

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Auteurs

Misao Nakanishi (M)

Department of Obstetrics and Gynecology, Center Hospital, National Center for Global Health and Medicine, Tokyo, Japan.
Department of Obstetrics and Gynecology, Keio University Graduate School of Medicine, Tokyo, Japan.

Nobuaki Funahashi (N)

Department of Metabolic Disorder, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Division of Cancer Cell Research, Research Institute, Kanagawa Cancer Center, Kanagawa, Japan.

Hideoki Fukuoka (H)

Department of Innovation Research, Waseda University Comprehensive Research Organization, Tokyo, Japan.
Department of Progressive DOHaD Research, Fukushima Medical University, Fukushima, Japan.

Takao Nammo (T)

Department of Metabolic Disorder, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Department of Metabolic Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.

Yuichi Sato (Y)

Department of Obstetrics and Gynecology, Obstetrics and Gynecology Tatedebari Sato Hospital, Gunma, Japan.

Hajime Yoshihara (H)

Japan Community Health Care Organization, Sagamino Hospital Center of Perinatal Medicine, Kanagawa, Japan.

Hajime Oishi (H)

Department of Obstetrics and Gynecology, Center Hospital, National Center for Global Health and Medicine, Tokyo, Japan.

Mamoru Tanaka (M)

Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.

Tetsu Yano (T)

Department of Obstetrics and Gynecology, Center Hospital, National Center for Global Health and Medicine, Tokyo, Japan.
Department of Obstetrics and Gynecology, Tokyo Yamate Medical Center, Tokyo, Japan.

Shigeki Minoura (S)

Department of Obstetrics and Gynecology, Center Hospital, National Center for Global Health and Medicine, Tokyo, Japan.
Department of Obstetrics and Gynecology, Shinjuku City Medical Association Residents' Health Center, Tokyo, Japan.

Norihiro Kato (N)

Department of Gene Diagnostics and Therapeutics, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.

Kazuki Yasuda (K)

Department of Metabolic Disorder, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Department of Diabetes, Endocrinology and Metabolism, Kyorin University School of Medicine, Tokyo, Japan.

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