Redundant type II cadherins define neuroepithelial cell states for cytoarchitectonic robustness.
Animals
CRISPR-Cas Systems
Cadherins
/ genetics
Cell Differentiation
/ genetics
Chromosome Mapping
Embryonic Development
/ genetics
Fluorescent Antibody Technique
Gene Editing
Gene Expression Regulation, Developmental
Gene Targeting
Genomics
/ methods
Humans
Immunohistochemistry
Mice
Neural Plate
/ embryology
Neural Tube
/ embryology
Neuroepithelial Cells
/ metabolism
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
15 10 2020
15 10 2020
Historique:
received:
17
11
2019
accepted:
15
09
2020
entrez:
16
10
2020
pubmed:
17
10
2020
medline:
22
6
2021
Statut:
epublish
Résumé
Individual cell shape and integrity must precisely be orchestrated during morphogenesis. Here, we determine function of type II cadherins, Cdh6, Cdh8, and Cdh11, whose expression combinatorially demarcates the mouse neural plate/tube. While CRISPR/Cas9-based single type II cadherin mutants show no obvious phenotype, Cdh6/8 double knockout (DKO) mice develop intermingled forebrain/midbrain compartments as these two cadherins' expression opposes at the nascent boundary. Cdh6/8/11 triple, Cdh6/8 or Cdh8/11 DKO mice further cause exencephaly just within the cranial region where mutated cadherins' expression merges. In the Cdh8/11 DKO midbrain, we observe less-constricted apical actin meshwork, ventrally-directed spreading, and occasional hyperproliferation among dorsal neuroepithelial cells as origins for exencephaly. These results provide rigid evidence that, by conferring distinct adhesive codes to each cell, redundant type II cadherins serve essential and shared roles in compartmentalization and neurulation, both of which proceed under the robust control of the number, positioning, constriction, and fluidity of neuroepithelial cells.
Identifiants
pubmed: 33060832
doi: 10.1038/s42003-020-01297-2
pii: 10.1038/s42003-020-01297-2
pmc: PMC7567090
doi:
Substances chimiques
Cadherins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
574Références
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Development. 2013 Jan 1;140(1):226-36
pubmed: 23175632
Development. 2002 Oct;129(20):4807-19
pubmed: 12361972
Dev Biol. 1999 Nov 15;215(2):182-9
pubmed: 10545229
Cell. 2014 Aug 14;158(4):793-807
pubmed: 25126785
Development. 2017 Feb 15;144(4):552-566
pubmed: 28196803
Dev Cell. 2013 Dec 23;27(6):621-34
pubmed: 24369835
Dev Biol. 2008 Mar 15;315(2):506-20
pubmed: 18234175
Development. 2019 Jul 10;146(13):
pubmed: 31235635
J Cell Biol. 1995 Apr;129(1):189-202
pubmed: 7698985
Nat Rev Genet. 2003 Oct;4(10):784-93
pubmed: 13679871
Science. 1996 Nov 15;274(5290):1109-15
pubmed: 8895453
Cell. 1999 Nov 24;99(5):485-97
pubmed: 10589677
Development. 2014 Sep;141(17):3303-18
pubmed: 25139853
J Neurosci. 2006 Feb 22;26(8):2147-56
pubmed: 16495441
Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):855-9
pubmed: 7846066
Cell. 1997 Oct 3;91(1):127-38
pubmed: 9335341
Proc Natl Acad Sci U S A. 2009 Jul 14;106(28):11594-9
pubmed: 19553217
Nat Biotechnol. 2010 May;28(5):511-5
pubmed: 20436464
Proc Natl Acad Sci U S A. 2014 Oct 7;111(40):E4175-84
pubmed: 25253890
Cell. 2013 May 9;153(4):910-8
pubmed: 23643243
Cell. 2012 May 25;149(5):1084-97
pubmed: 22632972
Genome Biol. 2014;15(12):550
pubmed: 25516281
Dev Biol. 1996 Dec 15;180(2):413-23
pubmed: 8954714
Nature. 1993 Jun 17;363(6430):630-4
pubmed: 8510755
Cell. 2002 Apr 19;109(2):205-16
pubmed: 12007407
Neuron. 2018 Sep 19;99(6):1145-1154.e6
pubmed: 30197236
Nature. 1990 Mar 29;344(6265):431-5
pubmed: 2320110
Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2801-6
pubmed: 8610121
J Pathol. 2010 Jan;220(2):217-30
pubmed: 19918803
Dev Biol. 2000 Jul 1;223(1):38-53
pubmed: 10864459
Nat Cell Biol. 1999 Aug;1(4):E83-5
pubmed: 10559925
J Cell Biol. 1987 Dec;105(6 Pt 1):2501-10
pubmed: 3320048
Development. 1993 Sep;119(1):247-61
pubmed: 8275860
Nature. 1999 May 20;399(6733):267-71
pubmed: 10353250
Development. 2008 Aug;135(15):2489-503
pubmed: 18621990
Neuron. 1990 Apr;4(4):493-506
pubmed: 2322458
Cell. 1990 Apr 6;61(1):147-55
pubmed: 2317870
Development. 1990 Sep;110(1):97-104
pubmed: 2081473
Science. 2013 Mar 1;339(6123):1222002
pubmed: 23449594
Dev Dyn. 1998 Apr;211(4):338-51
pubmed: 9566953
Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5177-E5186
pubmed: 28607062
EMBO J. 1988 Dec 1;7(12):3679-84
pubmed: 3061804
Sci Rep. 2013 Nov 27;3:3355
pubmed: 24284873
Cell Rep. 2018 May 8;23(6):1840-1852
pubmed: 29742438
Science. 2018 Jul 13;361(6398):156-162
pubmed: 29853554
Nat Rev Genet. 2000 Oct;1(1):20-9
pubmed: 11262869
Science. 2012 Aug 17;337(6096):816-21
pubmed: 22745249
Dev Biol. 2000 Mar 15;219(2):373-83
pubmed: 10694429
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
Dev Biol. 1997 Jan 1;181(1):64-78
pubmed: 9015265
Development. 1998 Aug;125(15):2963-71
pubmed: 9655818
Development. 2001 Feb;128(4):561-9
pubmed: 11171339
Dev Cell. 2015 Jan 26;32(2):241-55
pubmed: 25625209
Development. 2002 Dec;129(24):5827-38
pubmed: 12421720
Wiley Interdiscip Rev Dev Biol. 2013 Mar-Apr;2(2):213-27
pubmed: 24009034
J Cell Biol. 1996 Nov;135(3):767-79
pubmed: 8909549
Nature. 1999 Jul 1;400(6739):77-81
pubmed: 10403252
Dev Biol. 1997 Mar 15;183(2):183-94
pubmed: 9126293
Science. 2013 Feb 15;339(6121):819-23
pubmed: 23287718
J Neurosci. 2007 Mar 28;27(13):3466-76
pubmed: 17392463
Dev Biol. 2010 Aug 15;344(2):941-7
pubmed: 20558153
Nature. 1997 Oct 30;389(6654):966-70
pubmed: 9353119
Neuron. 2017 Sep 27;96(1):160-176.e8
pubmed: 28957665