Successful activation of rat T lymphocytes by sperm specific antigens in vitro.
Animals
Antigen-Presenting Cells
/ immunology
Antigens
/ metabolism
Autoimmune Diseases
/ metabolism
Cell Proliferation
Cell Survival
Coculture Techniques
Cytokines
/ metabolism
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Homozygote
Immunity, Cellular
In Vitro Techniques
Lymphocyte Activation
Male
Orchitis
/ metabolism
Rats
Rats, Wistar
Spermatozoa
/ immunology
Spleen
/ cytology
T-Lymphocytes
/ cytology
Tumor Necrosis Factor-alpha
/ metabolism
Antigen activation
Culture
Rat
Sperm
T cell
Journal
The Journal of reproduction and development
ISSN: 1348-4400
Titre abrégé: J Reprod Dev
Pays: Japan
ID NLM: 9438792
Informations de publication
Date de publication:
22 Dec 2020
22 Dec 2020
Historique:
pubmed:
6
10
2020
medline:
12
10
2021
entrez:
5
10
2020
Statut:
ppublish
Résumé
Autoimmune orchitis is a condition related to cellular immunity. A disease model involving transfer of T lymphocytes activated by known antigens would be useful for defining pathogenical molecules. Since no method for activating rat T cells using specific antigens is available, we started the study to develop the method. T cells were collected from draining lymph nodes of immunized rats, then co-cultured with syngeneic splenocytes as antigen-presenting cells (APC) in antigen-supplemented medium (= stimulation). The cells were then incubated in medium without antigens and APC (= resting). Repetitive stimulation and resting increased the number of the T cells more than 100-fold. The antigen-specific activation was demonstrated by cell proliferation assay and ELISA assay for interferon gamma. Flow cytometry revealed that > 95% of the cells expressed tumor necrosis factor alpha, a cytokine responsible for autoimmune orchitis. The present method will provide a new procedure to evaluate antigenicity of sperm molecules.
Identifiants
pubmed: 33012735
doi: 10.1262/jrd.2020-106
pmc: PMC7768165
doi:
Substances chimiques
Antigens
0
Cytokines
0
Tumor Necrosis Factor-alpha
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
599-605Références
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