The Role of Vaccinium Myrtillus in the Prevention of Renal Injury in an Experimental Model of Ruptured Abdominal Aortic Aneurysm.
Aorta, Abdominal
Apoptosis
Laparotomy
Malondialdehyde
Oxidative Stress
Renal Artery
Sodium Chloride
Journal
Brazilian journal of cardiovascular surgery
ISSN: 1678-9741
Titre abrégé: Braz J Cardiovasc Surg
Pays: Brazil
ID NLM: 101677045
Informations de publication
Date de publication:
01 08 2020
01 08 2020
Historique:
entrez:
1
9
2020
pubmed:
1
9
2020
medline:
26
1
2021
Statut:
epublish
Résumé
To examine the biochemical and histopathological renal effects of ischemia/reperfusion (I/R) injury using a ruptured abdominal aortic aneurysm (RAAA) model in rats and to investigate the potential protective effects of whortleberry (Vaccinium myrtillus). Thirty-two male Sprague-Dawley rats were randomly assigned into four groups - control, sham (I/R+glycerol), I/R, and I/R+whortleberry. Midline laparotomy alone was performed in the control group. Atraumatic abdominal clamps were attached under anesthesia to the abdominal aorta beneath the level of the renal artery in the groups subjected to I/R. Sixty-minute reperfusion was established one hour after ischemia. The sham group received five intraperitoneal doses of glycerol five days before I/R. The I/R+whortleberry group received a single intraperitoneal 50 mg/kg dose diluted with saline solution five days before I/R. All animals were finally euthanized by cervical dislocation following 60-min reperfusion. Increases were observed in malondialdehyde (MDA) levels and tubular necrosis scores (TNS) in thin kidney tissues and in numbers of apoptotic renal tubule cells, together with a decrease in glutathione (GSH) levels, in sham and I/R groups. In contrast, we observed a decrease in MDA levels, TNS, and numbers of apoptotic renal tubule cells, and an increase in GSH levels with whortleberry treatment compared to the I/R group. Our findings suggest that whortleberry may be effective against acute kidney injury by reducing oxidative stress and apoptosis.
Identifiants
pubmed: 32864929
doi: 10.21470/1678-9741-2019-0121
pmc: PMC7454617
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
490-497Références
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