Diversity and classification of cyclic-oligonucleotide-based anti-phage signalling systems.
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
19
03
2020
accepted:
16
07
2020
pubmed:
26
8
2020
medline:
27
2
2021
entrez:
26
8
2020
Statut:
ppublish
Résumé
Cyclic-oligonucleotide-based anti-phage signalling systems (CBASS) are a family of defence systems against bacteriophages (hereafter phages) that share ancestry with the cGAS-STING innate immune pathway in animals. CBASS systems are composed of an oligonucleotide cyclase, which generates signalling cyclic oligonucleotides in response to phage infection, and an effector that is activated by the cyclic oligonucleotides and promotes cell death. Cell death occurs before phage replication is completed, therefore preventing the spread of phages to nearby cells. Here, we analysed 38,000 bacterial and archaeal genomes and identified more than 5,000 CBASS systems, which have diverse architectures with multiple signalling molecules, effectors and ancillary genes. We propose a classification system for CBASS that groups systems according to their operon organization, signalling molecules and effector function. Four major CBASS types were identified, sharing at least six effector subtypes that promote cell death by membrane impairment, DNA degradation or other means. We observed evidence of extensive gain and loss of CBASS systems, as well as shuffling of effector genes between systems. We expect that our classification and nomenclature scheme will guide future research in the developing CBASS field.
Identifiants
pubmed: 32839535
doi: 10.1038/s41564-020-0777-y
pii: 10.1038/s41564-020-0777-y
pmc: PMC7610970
mid: EMS126797
doi:
Substances chimiques
Bacterial Proteins
0
Oligonucleotides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1608-1615Subventions
Organisme : European Research Council
ID : 681203
Pays : International
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