Diabetic Cataract in Spontaneously Diabetic Torii Fatty Rats.


Journal

Journal of diabetes research
ISSN: 2314-6753
Titre abrégé: J Diabetes Res
Pays: England
ID NLM: 101605237

Informations de publication

Date de publication:
2020
Historique:
received: 15 05 2020
revised: 09 07 2020
accepted: 17 07 2020
entrez: 25 8 2020
pubmed: 25 8 2020
medline: 3 7 2021
Statut: epublish

Résumé

Spontaneously Diabetic Torii (SDT) fatty rat is a novel animal model of type 2 diabetes with obesity. SDT fatty rats develop hyperglycemia, dyslipidemia, and other diabetic complications including ocular disorders; however, diabetic cataract formation in SDT fatty rats has not been fully investigated. The aim of the current study was to investigate the characteristics of cataract in the SDT fatty rats. The mean body weight of SDT fatty rats is larger than that of age-matched Sprague-Dawley (SD) rats and control animals until 8 weeks of age, and thereafter the growing speed decreased until the end of observation at 16 weeks of age. Blood glucose levels in SDT fatty rats were significantly higher than those in SD rats throughout the observational period. Slit-lamp examination revealed that no rats showed cataract formation at 5 weeks of age; however, SDT fatty rats gradually developed cortical cataract and posterior subcapsular cataract, both of which are the common types of cataract in patients with type 2 diabetes. The levels of glucose, sorbitol, and fructose were higher in the lens tissues of SDT fatty rats in comparison with that of SD rats. Furthermore, the level of 4-hydroxynonenal (4-HNE) was higher in the lens of SDT fatty rats than in that of SD rats. By contrast, total glutathione (GSH) concentration was lower in the lens of SDT fatty rats than in that of SD rats. The present study demonstrated that the cataractogenesis in SDT fatty rats resembled human diabetic cataract formation, indicating that SDT fatty rats serve as a potential animal model in researches on human cataract associated with type 2 diabetes and obesity.

Identifiants

pubmed: 32832559
doi: 10.1155/2020/3058547
pmc: PMC7422424
doi:

Types de publication

Comparative Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

3058547

Informations de copyright

Copyright © 2020 Kasumi Kikuchi et al.

Déclaration de conflit d'intérêts

The authors KY and SM are employees of CLEA Japan, Inc. The other authors declare that there are no conflicts of interest regarding the publication of this paper.

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Auteurs

Kasumi Kikuchi (K)

Laboratory of Ocular Cell Biology & Visual Science, Japan.
Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Miyuki Murata (M)

Laboratory of Ocular Cell Biology & Visual Science, Japan.
Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Kousuke Noda (K)

Laboratory of Ocular Cell Biology & Visual Science, Japan.
Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Satoru Kase (S)

Laboratory of Ocular Cell Biology & Visual Science, Japan.
Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Yoshiaki Tagawa (Y)

Laboratory of Ocular Cell Biology & Visual Science, Japan.
Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Yasushi Kageyama (Y)

Tokyo Animal & Diet Department, CLEA Japan, Inc., Tokyo, Japan.

Masami Shinohara (M)

Tokyo Animal & Diet Department, CLEA Japan, Inc., Tokyo, Japan.

Tomohiko Sasase (T)

Biological/Pharmacological Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco Inc., Osaka, Japan.

Susumu Ishida (S)

Laboratory of Ocular Cell Biology & Visual Science, Japan.
Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

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