Temporal and Spatial Heterogeneity of Host Response to SARS-CoV-2 Pulmonary Infection.


Journal

medRxiv : the preprint server for health sciences
Titre abrégé: medRxiv
Pays: United States
ID NLM: 101767986

Informations de publication

Date de publication:
02 Aug 2020
Historique:
entrez: 9 8 2020
pubmed: 9 8 2020
medline: 9 8 2020
Statut: epublish

Résumé

The relationship of SARS-CoV-2 lung infection and severity of pulmonary disease is not fully understood. We analyzed autopsy specimens from 24 patients who succumbed to SARS-CoV-2 infection using a combination of different RNA and protein analytical platforms to characterize inter- and intra- patient heterogeneity of pulmonary virus infection. There was a spectrum of high and low virus cases that was associated with duration of disease and activation of interferon pathway genes. Using a digital spatial profiling platform, the virus corresponded to distinct spatial expression of interferon response genes and immune checkpoint genes demonstrating the intra-pulmonary heterogeneity of SARS-CoV-2 infection.

Identifiants

pubmed: 32766600
doi: 10.1101/2020.07.30.20165241
pmc: PMC7402055
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI081848
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA240924
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA207021
Pays : United States

Commentaires et corrections

Type : UpdateIn

Références

Cell Rep. 2020 Jul 7;32(1):107863
pubmed: 32610043
Arthritis Rheumatol. 2020 Jul;72(7):1059-1063
pubmed: 32293098
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
Nat Med. 2020 Jun;26(6):842-844
pubmed: 32398875
Cell. 2020 May 28;181(5):1016-1035.e19
pubmed: 32413319
Oncotarget. 2016 Oct 11;7(41):66822-66834
pubmed: 27572315
Lancet Infect Dis. 2020 Oct;20(10):1135-1140
pubmed: 32526193
J Immunol. 2006 Nov 15;177(10):7303-11
pubmed: 17082649
Science. 2020 Sep 4;369(6508):
pubmed: 32669297
Nat Biotechnol. 2020 May;38(5):586-599
pubmed: 32393914
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Lancet. 2020 Feb 15;395(10223):497-506
pubmed: 31986264
Cell Host Microbe. 2020 Jun 10;27(6):883-890.e2
pubmed: 32407669
Lancet. 2020 Mar 28;395(10229):1033-1034
pubmed: 32192578
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
Nat Biotechnol. 2019 Jul;37(7):773-782
pubmed: 31061481
J Clin Invest. 2020 May 1;130(5):2620-2629
pubmed: 32217835
Antiviral Res. 2020 Jul;179:104811
pubmed: 32360182
Lancet. 2020 May 30;395(10238):1695-1704
pubmed: 32401715
Genome Biol. 2014;15(12):550
pubmed: 25516281
Cell. 2015 Jan 15;160(1-2):48-61
pubmed: 25594174
J Virol. 2013 Sep;87(17):9633-42
pubmed: 23804634
Immunity. 2020 Aug 18;53(2):442-455.e4
pubmed: 32668194
Cell. 2020 May 28;181(5):1036-1045.e9
pubmed: 32416070
Cell Mol Immunol. 2020 May;17(5):541-543
pubmed: 32203186
N Engl J Med. 2020 Jul 17;:
pubmed: 32678530
Cell Host Microbe. 2020 Jun 10;27(6):879-882.e2
pubmed: 32359396
J Immunol. 1997 Dec 1;159(11):5201-5
pubmed: 9548457
Science. 2020 Aug 7;369(6504):718-724
pubmed: 32661059
Immunity. 2019 May 21;50(5):1317-1334.e10
pubmed: 30979687
PLoS One. 2014 Feb 25;9(2):e90003
pubmed: 24587180

Auteurs

Niyati Desai (N)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.

Azfar Neyaz (A)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.

Annamaria Szabolcs (A)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.

Angela R Shih (AR)

Department of Pathology, Boston, MA 02114, USA.

Jonathan H Chen (JH)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.
Department of Pathology, Boston, MA 02114, USA.

Vishal Thapar (V)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.

Linda T Nieman (LT)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.

Alexander Solovyov (A)

Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Arnav Mehta (A)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.
The Broad Institute, Cambridge, MA 02142, USA.

David J Lieb (DJ)

The Broad Institute, Cambridge, MA 02142, USA.

Anupriya S Kulkarni (AS)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.

Christopher Jaicks (C)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.

Christopher J Pinto (CJ)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.

Dejan Juric (D)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.

Ivan Chebib (I)

Department of Pathology, Boston, MA 02114, USA.

Robert B Colvin (RB)

Department of Pathology, Boston, MA 02114, USA.

Arthur Y Kim (AY)

Department of Medicine, Boston, MA 02114, USA.

Robert Monroe (R)

Advanced Cell Diagnostics, a Bio-Techne Brand, Newark, CA 94560, USA.

Sarah E Warren (SE)

NanoString Inc., Seattle, WA 98109, USA.

Patrick Danaher (P)

NanoString Inc., Seattle, WA 98109, USA.

Jason W Reeves (JW)

NanoString Inc., Seattle, WA 98109, USA.

Jingjing Gong (J)

NanoString Inc., Seattle, WA 98109, USA.

Erroll H Rueckert (EH)

NanoString Inc., Seattle, WA 98109, USA.

Benjamin D Greenbaum (BD)

Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Nir Hacohen (N)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.
Department of Medicine, Boston, MA 02114, USA.
The Broad Institute, Cambridge, MA 02142, USA.

Stephen M Lagana (SM)

Columbia University Irving Medical Center, Department of Pathology and Cell Biology, New York, NY 10032, USA.

Miguel N Rivera (MN)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.
Department of Pathology, Boston, MA 02114, USA.
The Broad Institute, Cambridge, MA 02142, USA.

Lynette M Sholl (LM)

Brigham and Woman's Hospital, Department of Pathology, Boston, MA 02115.

James R Stone (JR)

Department of Pathology, Boston, MA 02114, USA.

David T Ting (DT)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.
Department of Medicine, Boston, MA 02114, USA.

Vikram Deshpande (V)

Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA.
Department of Pathology, Boston, MA 02114, USA.

Classifications MeSH