Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia.
Anemia, Aplastic
/ drug therapy
Angelica sinensis
/ chemistry
Animals
Apoptosis
/ drug effects
China
Disease Models, Animal
Male
Mice
Mice, Inbred BALB C
Mice, Inbred DBA
Mitochondria
/ pathology
Phytochemicals
/ pharmacology
Polysaccharides
/ pharmacology
T-Lymphocytes, Regulatory
/ cytology
Th17 Cells
/ cytology
Aplastic anemia
Apoptosis
Mitochondria
P38/MAPK
Th17
Treg
Journal
BMC complementary medicine and therapies
ISSN: 2662-7671
Titre abrégé: BMC Complement Med Ther
Pays: England
ID NLM: 101761232
Informations de publication
Date de publication:
22 Jun 2020
22 Jun 2020
Historique:
received:
08
07
2019
accepted:
16
06
2020
entrez:
24
6
2020
pubmed:
24
6
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Angelica sinensis polysaccharide (ASP) is an effective medicine for aplastic anemia (AA). The present study aims to investigate whether mitochondrial apoptosis in aplastic anemia could be corrected by ASP by adjusting an abnormal level of regulatory T cell (Treg)/ IL-17 secreting CD4 T cell (Th17) ratio. BALB/c mice were treated with 5.0 Gy Co60 γ -radiation. Then 2 × 10 The mice treated with the medium dose of ASP for 14 days showed increased white blood cell (WBC), red blood cell (RBC), platelet (PLT), BMNC counts and Lin-Sca-1 + c-Kit+ (LSK) populations viability compared with the mice in the AA group mice. The data showed that ASP decreased damage to the mitochondrial outer membrane, improved the stabilization of the mitochondrial membrane, and corrected the abnormal levels of ROS and mitochondrial-associated apoptosis proteins, including the Bcl-2/Bax ratio and caspase-3 and caspase-9 expression, in BMNCs which were sorted from the bone marrow cells of AA mice. The changes to the p-P38/P38 and Treg/Th17 ratios induced by AA were also reversed by the medium dose of ASP. The same ASP effect including the Bcl-2/Bax and p-P38/P38 ratio, caspase-3 and caspase-9 expression of BMNCs were observed in vivo. The viability of Treg cells were increased by treatment of ASP in vivo. ASP might prevent mitochondrial apoptosis to restore the function of hematopoietic stem cells by suppressing abnormal T-cell immunity in AA.
Sections du résumé
BACKGROUND
BACKGROUND
Angelica sinensis polysaccharide (ASP) is an effective medicine for aplastic anemia (AA). The present study aims to investigate whether mitochondrial apoptosis in aplastic anemia could be corrected by ASP by adjusting an abnormal level of regulatory T cell (Treg)/ IL-17 secreting CD4 T cell (Th17) ratio.
METHODS
METHODS
BALB/c mice were treated with 5.0 Gy Co60 γ -radiation. Then 2 × 10
RESULTS
RESULTS
The mice treated with the medium dose of ASP for 14 days showed increased white blood cell (WBC), red blood cell (RBC), platelet (PLT), BMNC counts and Lin-Sca-1 + c-Kit+ (LSK) populations viability compared with the mice in the AA group mice. The data showed that ASP decreased damage to the mitochondrial outer membrane, improved the stabilization of the mitochondrial membrane, and corrected the abnormal levels of ROS and mitochondrial-associated apoptosis proteins, including the Bcl-2/Bax ratio and caspase-3 and caspase-9 expression, in BMNCs which were sorted from the bone marrow cells of AA mice. The changes to the p-P38/P38 and Treg/Th17 ratios induced by AA were also reversed by the medium dose of ASP. The same ASP effect including the Bcl-2/Bax and p-P38/P38 ratio, caspase-3 and caspase-9 expression of BMNCs were observed in vivo. The viability of Treg cells were increased by treatment of ASP in vivo.
CONCLUSIONS
CONCLUSIONS
ASP might prevent mitochondrial apoptosis to restore the function of hematopoietic stem cells by suppressing abnormal T-cell immunity in AA.
Identifiants
pubmed: 32571324
doi: 10.1186/s12906-020-02995-4
pii: 10.1186/s12906-020-02995-4
pmc: PMC7309996
doi:
Substances chimiques
Phytochemicals
0
Polysaccharides
0
Types de publication
Journal Article
Retracted Publication
Langues
eng
Sous-ensembles de citation
IM
Pagination
192Subventions
Organisme : National Natural Science Foundation of China
ID : 81202839
Organisme : Taishan Scholar Project of Shandong Province
ID : tsqn201812145
Commentaires et corrections
Type : RetractionIn
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