C57BL/6 substrain differences in formalin-induced pain-like behavioral responses.

Animals Behavior, Animal / physiology Disinfectants / pharmacology Female Formaldehyde / pharmacology Hyperalgesia / chemically induced Inflammation / chemically induced Male Mice Mice, Inbred C57BL / physiology Nociceptive Pain / chemically induced
C57BL/6 substrains Formalin model Genetic variability Inflammatory pain Reflexive tests Rodents

Journal

Behavioural brain research
ISSN: 1872-7549
Titre abrégé: Behav Brain Res
Pays: Netherlands
ID NLM: 8004872

Informations de publication

Date de publication:
15 07 2020
Historique:
received: 24 03 2020
revised: 04 05 2020
accepted: 07 05 2020
pubmed: 20 5 2020
medline: 26 10 2021
entrez: 20 5 2020
Statut: ppublish

Résumé

Substantial evidence from preclinical models of pain suggests that basal and noxious nociceptive sensitivity, as well as antinociceptive responses to drugs, show significant heritability. Individual differences to these responses have been observed across species from rodents to humans. The use of closely related C57BL/6 inbred mouse substrains can facilitate gene mapping of acute nociceptive behaviors in preclinical pain models. In this study, we investigated behavioral differences between C57BL/6 J (B6 J) and C57BL/6 N (B6 N) substrains in the formalin test, a widely used tonic inflammatory pain model, using a battery of pain-related phenotypes, including reflexive tests, nesting, voluntary wheel running, sucrose preference and anxiety-like behavior in the light/dark test at two different time points (1-h and 24-h). Our results show that these substrains did not differ in reflexive thermal and mechanical responses at the 1-h time point. However, B6 N substrain mice showed increased sensitivity to spontaneous pain-like behaviors. In addition, B6 N substrain continued to show higher levels of mechanical hypersensitivity compared to controls at 24-h. indicating that mechanical hypersensitivity is a more persistent pain-related phenotype induced by formalin. Finally, no sex differences were observed in our outcome measures. Our results provide a comprehensive behavioral testing paradigm in response to an inflammatory agent for future mouse genetic studies in pain.

Identifiants

DOI: 10.1016/j.bbr.2020.112698 PMID: 32428630 PMC: PMC7375808
pubmed: 32428630
pii: S0166-4328(20)30397-1
doi: 10.1016/j.bbr.2020.112698
pmc: PMC7375808
mid: NIHMS1595169
pii:
doi:

Substances chimiques

Disinfectants 0
Formaldehyde 1HG84L3525

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

112698

Subventions

Organisme : NCI NIH HHS
ID : R01 CA221260
Pays : United States
Organisme : NIDA NIH HHS
ID : T32 DA007027
Pays : United States

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

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Auteurs

Esad Ulker (E)

Department of Pharmacology and Toxicology and Translational Research Initiative for Pain and Neuropathy, Virginia Commonwealth University, Virginia Commonwealth University, Richmond, VA 23298-0613, USA. Electronic address: esad.ulker@vcuhealth.org.

Martial Caillaud (M)

Department of Pharmacology and Toxicology and Translational Research Initiative for Pain and Neuropathy, Virginia Commonwealth University, Virginia Commonwealth University, Richmond, VA 23298-0613, USA.

Trusha Patel (T)

Department of Pharmacology and Toxicology and Translational Research Initiative for Pain and Neuropathy, Virginia Commonwealth University, Virginia Commonwealth University, Richmond, VA 23298-0613, USA.

Alyssa White (A)

Department of Pharmacology and Toxicology and Translational Research Initiative for Pain and Neuropathy, Virginia Commonwealth University, Virginia Commonwealth University, Richmond, VA 23298-0613, USA.

Danyal Rashid (D)

Department of Pharmacology and Toxicology and Translational Research Initiative for Pain and Neuropathy, Virginia Commonwealth University, Virginia Commonwealth University, Richmond, VA 23298-0613, USA.

Mashael Alqasem (M)

Department of Pharmacology and Toxicology and Translational Research Initiative for Pain and Neuropathy, Virginia Commonwealth University, Virginia Commonwealth University, Richmond, VA 23298-0613, USA.

Aron H Lichtman (AH)

Department of Pharmacology and Toxicology and Translational Research Initiative for Pain and Neuropathy, Virginia Commonwealth University, Virginia Commonwealth University, Richmond, VA 23298-0613, USA.

Camron D Bryant (CD)

Laboratory of Addiction Genetics, Department of Pharmacology and Experimental Therapeutics and Psychiatry, Boston University School of Medicine, Boston, MA, 02118, USA.

M Imad Damaj (MI)

Department of Pharmacology and Toxicology and Translational Research Initiative for Pain and Neuropathy, Virginia Commonwealth University, Virginia Commonwealth University, Richmond, VA 23298-0613, USA.

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Classifications MeSH

Recherchemedicale.com › Eucaryotes › Animaux › C57BL/6 substrain differences in...
Recherchemedicale.com › Comportement et mécanismes comportementaux › Comportement › Comportement animal › C57BL/6 substrain differences in...
Recherchemedicale.com › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Désinfectants › C57BL/6 substrain differences in...
Recherchemedicale.com › Composés chimiques organiques › Aldéhydes › Formaldéhyde › C57BL/6 substrain differences in...
Recherchemedicale.com › États, signes et symptômes pathologiques › Signes et symptômes › Manifestations neurologiques › Troubles sensitifs › Troubles somatosensoriels › Hyperalgésie › C57BL/6 substrain differences in...
Recherchemedicale.com › États, signes et symptômes pathologiques › Processus pathologiques › Inflammation › C57BL/6 substrain differences in...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › C57BL/6 substrain differences in...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › C57BL/6 substrain differences in...
Recherchemedicale.com › États, signes et symptômes pathologiques › Signes et symptômes › Manifestations neurologiques › Douleur nociceptive › C57BL/6 substrain differences in...