A conserved and regulated mechanism drives endosomal Rab transition.
Animals
Casein Kinase I
/ metabolism
Drosophila
Drosophila Proteins
/ metabolism
Endosomes
/ metabolism
Guanine Nucleotide Exchange Factors
/ metabolism
Liposomes
/ metabolism
Membrane Fusion
Phosphatidylinositol Phosphates
/ metabolism
Phosphorylation
Protein Binding
Protein Prenylation
Saccharomyces cerevisiae Proteins
/ metabolism
Sf9 Cells
Vacuoles
/ metabolism
Vesicular Transport Proteins
/ metabolism
rab GTP-Binding Proteins
/ genetics
rab5 GTP-Binding Proteins
/ genetics
rab7 GTP-Binding Proteins
D. melanogaster
GEF
Mon1-Ccz1
Rab cascade
Rab5
Rab7
S. cerevisiae
biochemistry
cell biology
chemical biology
endosome
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
11 05 2020
11 05 2020
Historique:
received:
17
02
2020
accepted:
07
05
2020
pubmed:
12
5
2020
medline:
17
3
2021
entrez:
12
5
2020
Statut:
epublish
Résumé
Endosomes and lysosomes harbor Rab5 and Rab7 on their surface as key proteins involved in their identity, biogenesis, and fusion. Rab activation requires a guanine nucleotide exchange factor (GEF), which is Mon1-Ccz1 for Rab7. During endosome maturation, Rab5 is replaced by Rab7, though the underlying mechanism remains poorly understood. Here, we identify the molecular determinants for Rab conversion in vivo and in vitro, and reconstitute Rab7 activation with yeast and metazoan proteins. We show (i) that Mon1-Ccz1 is an effector of Rab5, (ii) that membrane-bound Rab5 is the key factor to directly promote Mon1-Ccz1 dependent Rab7 activation and Rab7-dependent membrane fusion, and (iii) that this process is regulated in yeast by the casein kinase Yck3, which phosphorylates Mon1 and blocks Rab5 binding. Our study thus uncovers the minimal feed-forward machinery of the endosomal Rab cascade and a novel regulatory mechanism controlling this pathway.
Identifiants
pubmed: 32391792
doi: 10.7554/eLife.56090
pii: 56090
pmc: PMC7239660
doi:
pii:
Substances chimiques
Ccz1 protein, S cerevisiae
0
Drosophila Proteins
0
Guanine Nucleotide Exchange Factors
0
Liposomes
0
Mon1 protein, S cerevisiae
0
Phosphatidylinositol Phosphates
0
Saccharomyces cerevisiae Proteins
0
Vesicular Transport Proteins
0
phosphatidylinositol 3-phosphate
0
rab7 GTP-Binding Proteins
0
Casein Kinase I
EC 2.7.11.1
YCK3 protein, S cerevisiae
EC 2.7.11.1
rab GTP-Binding Proteins
EC 3.6.5.2
rab5 GTP-Binding Proteins
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB 944
Pays : International
Informations de copyright
© 2020, Langemeyer et al.
Déclaration de conflit d'intérêts
LL, AB, EH, NF, YH, AP, KA, DK, CU No competing interests declared
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