The Antimicrobial Peptide LL-37 Promotes Migration and Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla through the Akt/Wnt/β-catenin Signaling Pathway.
Akt
LL-37
Wnt/β-catenin pathways
odonto/osteogenic differentiation
stem cells from the apical papilla
Journal
Journal of endodontics
ISSN: 1878-3554
Titre abrégé: J Endod
Pays: United States
ID NLM: 7511484
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
15
09
2019
revised:
22
02
2020
accepted:
10
03
2020
pubmed:
12
5
2020
medline:
18
9
2020
entrez:
12
5
2020
Statut:
ppublish
Résumé
The antimicrobial peptide LL-37, in addition to its broad spectrum of antibacterial function, can promote odontogenesis and osteogenesis. Stem cells from the apical papilla (SCAPs) are essential for the formation of dentin/bonelike tissues. However, little information on these cells is available in regenerative endodontics. This study aimed to evaluate the effects of LL-37 on the proliferation, migration, and differentiation of SCAPs. SCAPs were isolated, cultured, and characterized. Cell viability was analyzed by Cell Counting Kit-8 assays (Dojindo, Kumamoto, Japan). Cell migration was investigated by transwell assays. Dentin sialophosphoprotein, dentin matrix protein 1, runt-related transcription factor 2, and osterix were assessed by quantitative polymerase chain reaction and Western blots. Alkaline phosphatase (ALP) activity and ALP staining were assessed to determine the in vitro potential for osteogenic differentiation. The involvement of the Akt/Wnt/β-catenin signaling pathway was also studied. In the 2.5-μg/mL LL-37 -treated group, cell proliferation and migration were up-regulated. Quantitative polymerase chain reaction and Western blot assays both revealed that LL-37 at 2.5 μg/mL up-regulated odonto/osteogenic markers (dentin sialophosphoprotein, dentin matrix protein 1, runt-related transcription factor 2, and osterix). LL-37 at 2.5 μg/mL significantly promoted ALP activity and increased the staining in SCAPs. In addition, the p-Akt and p-glycogen synthase kinase-3β levels were increased in LL-37-treated SCAPs. The migratory and odonto/osteogenic differentiation capacities of SCAPs were inhibited after treatment with inhibitors LY294002 and XAV-939. Our study showed that LL-37 at 2.5 μg/mL promoted the migration and odonto/osteogenic differentiation of SCAPs by activating the Akt/Wnt/β-catenin signaling pathway.
Identifiants
pubmed: 32389381
pii: S0099-2399(20)30194-1
doi: 10.1016/j.joen.2020.03.013
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Antimicrobial Cationic Peptides
0
Cathelicidins
0
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Pagination
964-972Informations de copyright
Copyright © 2020. Published by Elsevier Inc.