Abnormal Reinnervation of Denervated Areas Following Nerve Injury Facilitates Neuropathic Pain.
PNS
SNI
denervation
neuropathic pain
nociceptive
non-nociceptive
ouabain
reinnervation
retrograde labeling
sprouting
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
18 04 2020
18 04 2020
Historique:
received:
17
03
2020
revised:
10
04
2020
accepted:
14
04
2020
entrez:
25
4
2020
pubmed:
25
4
2020
medline:
30
3
2021
Statut:
epublish
Résumé
An injury to peripheral nerves leads to skin denervation, which often is followed by increased pain sensitivity of the denervated areas and the development of neuropathic pain. Changes in innervation patterns during the reinnervation process of the denervated skin could contribute to the development of neuropathic pain. Here, we examined the changes in the innervation pattern during reinnervation and correlated them with the symptoms of neuropathic pain. Using a multispectral labeling technique-PainBow, which we developed, we characterized dorsal root ganglion (DRG) neurons innervating distinct areas of the rats' paw. We then used spared nerve injury, causing partial denervation of the paw, and examined the changes in innervation patterns of the denervated areas during the development of allodynia and hyperalgesia. We found that, differently from normal conditions, during the development of neuropathic pain, these areas were mainly innervated by large, non-nociceptive neurons. Moreover, we found that the development of neuropathic pain is correlated with an overall decrease in the number of DRG neurons innervating these areas. Importantly, treatment with ouabain facilitated reinnervation and alleviated neuropathic pain. Our results suggest that local changes in peripheral innervation following denervation contribute to neuropathic pain development. The reversal of these changes decreases neuropathic pain.
Identifiants
pubmed: 32325693
pii: cells9041007
doi: 10.3390/cells9041007
pmc: PMC7226396
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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