Histological correlates of postmortem ultra-high-resolution single-section MRI in cortical cerebral microinfarcts.
Acute Disease
Aged
Aged, 80 and over
Antigens, CD
/ metabolism
Antigens, Differentiation, Myelomonocytic
/ metabolism
Astrocytes
/ metabolism
Autopsy
Cerebral Cortex
/ diagnostic imaging
Cerebral Infarction
/ diagnostic imaging
Cerebral Small Vessel Diseases
/ diagnostic imaging
Chronic Disease
Collagen Type IV
/ metabolism
Dementia
/ diagnostic imaging
Female
Glial Fibrillary Acidic Protein
/ metabolism
Humans
Macrophages
/ metabolism
Magnetic Resonance Imaging
Male
Microvessels
/ metabolism
Parkinson Disease
/ diagnostic imaging
Cerebral microangiopathy
Histological matching
Microbleeds
Post-mortem magnetic resonance imaging
Small vessel disease
String vessels
Journal
Acta neuropathologica communications
ISSN: 2051-5960
Titre abrégé: Acta Neuropathol Commun
Pays: England
ID NLM: 101610673
Informations de publication
Date de publication:
13 03 2020
13 03 2020
Historique:
received:
25
12
2019
accepted:
21
02
2020
entrez:
15
3
2020
pubmed:
15
3
2020
medline:
16
1
2021
Statut:
epublish
Résumé
The identification of cerebral microinfarctions with magnetic resonance imaging (MRI) and histological methods remains challenging in aging and dementia. Here, we matched pathological changes in the microvasculature of cortical cerebral microinfarcts to MRI signals using single 100 μm-thick histological sections scanned with ultra-high-resolution 11.7 T MRI. Histologically, microinfarcts were located in superficial or deep cortical layers or transcortically, compatible with the pattern of layer-specific arteriolar blood supply of the cerebral cortex. Contrary to acute microinfarcts, at chronic stages the core region of microinfarcts showed pallor with extracellular accumulation of lipofuscin and depletion of neurons, a dense meshwork of collagen 4-positive microvessels with numerous string vessels, CD68-positive macrophages and glial fibrillary acidic protein (GFAP)-positive astrocytes. In MRI scans, cortical microinfarcts at chronic stages, called chronic cortical microinfarcts here, gave hypointense signals in T1-weighted and hyperintense signals in T2-weighted images when thinning of the tissue and cavitation and/or prominent iron accumulation were present. Iron accumulation in chronic microinfarcts, histologically verified with Prussian blue staining, also produced strong hypointense T2*-weighted signals. In summary, the microinfarct core was occupied by a dense microvascular meshwork with string vessels, which was invaded by macrophages and astroglia and contained various degrees of iron accumulation. While postmortem ultra-high-resolution single-section imaging improved MRI-histological matching and the structural characterization of chronic cortical cerebral microinfarcts, miniscule microinfarcts without thinning or iron accumulation could not be detected with certainty in the MRI scans. Moreover, string vessels at the infarct margin indicate disturbances in the microcirculation in and around microinfarcts, which might be exploitable in the diagnostics of cortical cerebral microinfarcts with MRI in vivo.
Identifiants
pubmed: 32169123
doi: 10.1186/s40478-020-00900-1
pii: 10.1186/s40478-020-00900-1
pmc: PMC7071593
doi:
Substances chimiques
Antigens, CD
0
Antigens, Differentiation, Myelomonocytic
0
CD68 antigen, human
0
Collagen Type IV
0
GFAP protein, human
0
Glial Fibrillary Acidic Protein
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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