Analysis of mitochondrial enzymatic activity in blood lymphomonocyte fractions during infection with different Trypanosoma cruzi strains.
Journal
Revista do Instituto de Medicina Tropical de Sao Paulo
ISSN: 1678-9946
Titre abrégé: Rev Inst Med Trop Sao Paulo
Pays: Brazil
ID NLM: 7507484
Informations de publication
Date de publication:
2020
2020
Historique:
received:
25
09
2019
accepted:
02
12
2019
entrez:
20
2
2020
pubmed:
20
2
2020
medline:
11
4
2020
Statut:
epublish
Résumé
Proinflammatory and inflammatory mediators induced by Trypanosoma cruzi infection increase the oxidative stress, generating toxicity for cells targeting mitochondria of different tissues. We studied the activity of citrate synthase and complexes I-IV of respiratory chain in mitochondria of blood lymphomonocyte fraction, from albino Swiss mice infected with different isolates of T. cruzi , during Chagas disease evolution. Complexes I-IV were modified in infected groups (p<0.05) in all the stages, and an inflammatory process of different magnitudes was detected in the heart and skeletal muscle according to the isolate. The citrate synthase activity presented modifications in the SGO Z12 and the Tulahuen group (p<0.05). Hearts showed fiber fragmentation and fibrosis; skeletal muscle presented inflammatory infiltrates and in the Tulahuen infected group, there were also amastigote nests. The inflammatory processes produced an oxidative stress that induced different alterations of mitochondrial enzymes activities in the lymphomonocyte fraction that can be detected by a simple blood extraction, suggesting that they could be used as disease markers, especially in the indeterminate phase of Chagas disease.
Identifiants
pubmed: 32074218
pii: S0036-46652020000100208
doi: 10.1590/S1678-9946202062015
pmc: PMC7032009
pii:
doi:
Substances chimiques
Electron Transport Complex IV
EC 1.9.3.1
Citrate (si)-Synthase
EC 2.3.3.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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