ZGLP1 is a determinant for the oogenic fate in mice.
Adaptor Proteins, Signal Transducing
/ genetics
Animals
Bone Morphogenetic Proteins
/ metabolism
Female
Fetus
/ cytology
Gene Expression Regulation, Developmental
Male
Meiosis
/ genetics
Mice
Mice, Knockout
Oocytes
/ cytology
Oogenesis
/ genetics
Polycomb-Group Proteins
/ metabolism
Repressor Proteins
/ genetics
Sex Determination Processes
Sex Differentiation
/ genetics
Signal Transduction
Transcription Factors
/ genetics
Transcriptome
Tretinoin
/ physiology
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
06 03 2020
06 03 2020
Historique:
received:
18
12
2018
revised:
17
10
2019
accepted:
31
01
2020
pubmed:
15
2
2020
medline:
24
3
2020
entrez:
15
2
2020
Statut:
ppublish
Résumé
Sex determination of germ cells is vital to creating the sexual dichotomy of germ cell development, thereby ensuring sexual reproduction. However, the underlying mechanisms remain unclear. Here, we show that ZGLP1, a conserved transcriptional regulator with GATA-like zinc fingers, determines the oogenic fate in mice. ZGLP1 acts downstream of bone morphogenetic protein, but not retinoic acid (RA), and is essential for the oogenic program and meiotic entry. ZGLP1 overexpression induces differentiation of in vitro primordial germ cell-like cells (PGCLCs) into fetal oocytes by activating the oogenic programs repressed by Polycomb activities, whereas RA signaling contributes to oogenic program maturation and PGC program repression. Our findings elucidate the mechanism for mammalian oogenic fate determination, providing a foundation for promoting in vitro gametogenesis and reproductive medicine.
Identifiants
pubmed: 32054698
pii: science.aaw4115
doi: 10.1126/science.aaw4115
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Bone Morphogenetic Proteins
0
Polycomb-Group Proteins
0
Repressor Proteins
0
Stra8 protein, mouse
0
Transcription Factors
0
Zglp1 protein, mouse
0
Tretinoin
5688UTC01R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.