Engineering a Nanostructured Nucleolin-Binding Peptide for Intracellular Drug Delivery in Triple-Negative Breast Cancer Stem Cells.
ADP Ribose Transferases
/ chemistry
Antineoplastic Agents
/ chemistry
Bacterial Toxins
/ chemistry
Cell Line, Tumor
Cell Survival
/ drug effects
Drug Carriers
/ chemistry
Exotoxins
/ chemistry
Female
Humans
Nanostructures
/ chemistry
Neoplastic Stem Cells
/ cytology
Peptides
/ chemistry
Pseudomonas aeruginosa
/ metabolism
Receptors, Cell Surface
/ chemistry
Triple Negative Breast Neoplasms
/ metabolism
Virulence Factors
/ chemistry
Pseudomonas aeruginosa Exotoxin A
cancer stem cells
nucleolin
self-assembling polypeptides
targeting
triple-negative breast cancer
Journal
ACS applied materials & interfaces
ISSN: 1944-8252
Titre abrégé: ACS Appl Mater Interfaces
Pays: United States
ID NLM: 101504991
Informations de publication
Date de publication:
05 Feb 2020
05 Feb 2020
Historique:
pubmed:
17
12
2019
medline:
12
11
2020
entrez:
17
12
2019
Statut:
ppublish
Résumé
Five peptide ligands of four different cell surface receptors (nucleolin, CXCR1, CMKLR1, and CD44v6) have been evaluated as targeting moieties for triple-negative human breast cancers. Among them, the peptide F3, derived from phage display, promotes the fast and efficient internalization of a genetically fused green fluorescent protein (GFP) inside MDA-MB-231 cancer stem cells in a specific receptor-dependent fashion. The further engineering of this protein into the modular construct F3-RK-GFP-H6 and the subsequent construct F3-RK-PE24-H6 resulted in self-assembling polypeptides that organize as discrete and regular nanoparticles. These materials, 15-20 nm in size, show enhanced nucleolin-dependent cell penetrability. We show that the F3-RK-PE24-H6, based on the
Identifiants
pubmed: 31840972
doi: 10.1021/acsami.9b15803
doi:
Substances chimiques
Antineoplastic Agents
0
Bacterial Toxins
0
Drug Carriers
0
Exotoxins
0
Peptides
0
Receptors, Cell Surface
0
Virulence Factors
0
nucleolin binding peptide
0
ADP Ribose Transferases
EC 2.4.2.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM