Mycobacterium tuberculosis HN878 Infection Induces Human-Like B-Cell Follicles in Mice.
Mycobacterium tuberculosis HN878
B-cell follicles
human granulomatous diseases
lung granuloma
pulmonary tuberculosis
Journal
The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675
Informations de publication
Date de publication:
27 04 2020
27 04 2020
Historique:
received:
20
08
2019
accepted:
11
12
2019
pubmed:
14
12
2019
medline:
7
2
2021
entrez:
14
12
2019
Statut:
ppublish
Résumé
Specific spatial organization of granulomas within the lungs is crucial for protective anti-tuberculosis (TB) immune responses. However, only large animal models such as macaques are thought to reproduce the morphological hallmarks of human TB granulomas. In this study, we show that infection of mice with clinical "hypervirulent" Mycobacterium tuberculosis (Mtb) HN878 induces human-like granulomas composed of bacilli-loaded macrophages surrounded by lymphocytes and organized localization of germinal centers and B-cell follicles. Infection with laboratory-adapted Mtb H37Rv resulted in granulomas that are characterized by unorganized clusters of macrophages scattered between lymphocytes. An in-depth exploration of the functions of B cells within these follicles suggested diverse roles and the activation of signaling pathways associated with antigen presentation and immune cell recruitment. These findings support the use of clinical Mtb HN878 strain for infection in mice as an appropriate model to study immune parameters associated with human TB granulomas.
Identifiants
pubmed: 31832640
pii: 5674951
doi: 10.1093/infdis/jiz663
pmc: PMC7184917
doi:
Substances chimiques
Immunoglobulin mu-Chains
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1636-1646Subventions
Organisme : NIAID NIH HHS
ID : R01 AI123780
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI111914
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL007317
Pays : United States
Organisme : NIH HHS
ID : P51 OD011104
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL105427
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI134240
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI134236
Pays : United States
Informations de copyright
© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.
Références
Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16607-12
pubmed: 20807742
PLoS One. 2017 Mar 17;12(3):e0173715
pubmed: 28306733
Nat Med. 2014 Jan;20(1):75-9
pubmed: 24336248
J Infect Dis. 2007 Jan 1;195(1):46-54
pubmed: 17152008
Nature. 1998 Jun 11;393(6685):537-44
pubmed: 9634230
Nucleic Acids Res. 2018 Jan 4;46(D1):D754-D761
pubmed: 29155950
Infect Immun. 2008 Jun;76(6):2333-40
pubmed: 18347040
J Immunol. 2007 Jul 1;179(1):522-31
pubmed: 17579073
Nucleic Acids Res. 2018 Jan 4;46(D1):D649-D655
pubmed: 29145629
Glob Vaccines Immunol. 2016 Jun;1(2):44-52
pubmed: 30271881
Tuberculosis (Edinb). 2016 Mar;97:8-17
pubmed: 26980490
Front Immunol. 2017 Apr 06;8:414
pubmed: 28428789
Bioinformatics. 2014 Apr 1;30(7):923-30
pubmed: 24227677
Cell Microbiol. 2006 Feb;8(2):218-32
pubmed: 16441433
PLoS Pathog. 2014 May 15;10(5):e1004099
pubmed: 24831696
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
J Biol Chem. 2003 Dec 26;278(52):52739-46
pubmed: 14525980
Nucleic Acids Res. 2017 Jul 3;45(W1):W130-W137
pubmed: 28472511
Immunol Rev. 2015 Mar;264(1):288-307
pubmed: 25703567
JCI Insight. 2017 Oct 5;2(19):
pubmed: 28978810
Clin Dev Immunol. 2012;2012:139127
pubmed: 22811737
Mucosal Immunol. 2018 Nov;11(6):1727-1742
pubmed: 30115997
Genome Biol. 2010;11(10):R106
pubmed: 20979621
J Immunol. 2009 Dec 15;183(12):8004-14
pubmed: 19933855
Mucosal Immunol. 2015 Sep;8(5):1099-109
pubmed: 25627812
Am J Respir Cell Mol Biol. 1992 Jun;6(6):594-600
pubmed: 1591008
Immunobiology. 2016 Apr;221(4):558-67
pubmed: 26719096
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
Proc Natl Acad Sci U S A. 2001 May 8;98(10):5752-7
pubmed: 11320211
J Immunol. 2007 Jun 1;178(11):7222-34
pubmed: 17513771
J Clin Invest. 2013 Feb;123(2):712-26
pubmed: 23281399