Suppression of Presynaptic Glutamate Release by Postsynaptic Metabotropic NMDA Receptor Signalling to Pannexin-1.
Animals
Calcium Chelating Agents
/ pharmacology
Connexins
/ metabolism
Egtazic Acid
/ analogs & derivatives
Excitatory Postsynaptic Potentials
/ drug effects
Glutamic Acid
/ metabolism
Hippocampus
/ drug effects
Mice
Mice, Knockout
Nerve Tissue Proteins
/ metabolism
Neurons
/ drug effects
Presynaptic Terminals
/ drug effects
Rats
Receptors, N-Methyl-D-Aspartate
/ metabolism
Signal Transduction
/ drug effects
Sodium Channel Blockers
/ pharmacology
Synapses
/ drug effects
TRPV Cation Channels
/ genetics
Tetrodotoxin
/ pharmacology
src-Family Kinases
/ metabolism
TRPV1
anandamide
glutamate transmission
hippocampus
metabotropic NMDAR
pannexin
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
ISSN: 1529-2401
Titre abrégé: J Neurosci
Pays: United States
ID NLM: 8102140
Informations de publication
Date de publication:
22 01 2020
22 01 2020
Historique:
received:
29
01
2019
revised:
26
11
2019
accepted:
03
12
2019
pubmed:
11
12
2019
medline:
24
7
2020
entrez:
11
12
2019
Statut:
ppublish
Résumé
The impact of pannexin-1 (Panx1) channels on synaptic transmission is poorly understood. Here, we show that selective block of Panx1 in single postsynaptic hippocampal CA1 neurons from male rat or mouse brain slices causes intermittent, seconds long increases in the frequency of sEPSC following Schaffer collateral stimulation. The increase in sEPSC frequency occurred without an effect on evoked neurotransmission. Consistent with a presynaptic origin of the augmented glutamate release, the increased sEPSC frequency was prevented by bath-applied EGTA-AM or TTX. Manipulation of a previously described metabotropic NMDAR pathway (i.e., by preventing ligand binding to NMDARs with competitive antagonists or blocking downstream Src kinase) also increased sEPSC frequency similar to that seen when Panx1 was blocked. This facilitated glutamate release was absent in transient receptor potential vanilloid 1 (TRPV1) KO mice and prevented by the TRPV1 antagonist, capsazepine, suggesting it required presynaptic TRPV1. We show presynaptic expression of TRPV1 by immunoelectron microscopy and link TRPV1 to Panx1 because Panx1 block increases tissue levels of the endovanilloid, anandamide. Together, these findings demonstrate an unexpected role for metabotropic NMDARs and postsynaptic Panx1 in suppression of facilitated glutamate neurotransmission.
Identifiants
pubmed: 31818976
pii: JNEUROSCI.0257-19.2019
doi: 10.1523/JNEUROSCI.0257-19.2019
pmc: PMC6975291
doi:
Substances chimiques
Calcium Chelating Agents
0
Connexins
0
Nerve Tissue Proteins
0
Panx1 protein, mouse
0
Receptors, N-Methyl-D-Aspartate
0
Sodium Channel Blockers
0
TRPV Cation Channels
0
TRPV1 protein, mouse
0
Glutamic Acid
3KX376GY7L
Tetrodotoxin
4368-28-9
Egtazic Acid
526U7A2651
EGTA acetoxymethyl ester
99590-86-0
src-Family Kinases
EC 2.7.10.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
729-742Subventions
Organisme : CIHR
ID : FDN 143329
Pays : Canada
Organisme : CIHR
ID : MOP 136812
Pays : Canada
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2020 the authors.
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